MiR-147b-3p promotes osteogenesis by targeting NDUFA4 and PI3K/AKT pathway

Journal of Orthopaedic Surgery and Research, Journal Year: 2025, Volume and Issue: 20(1)

Published: March 4, 2025

Osteoporosis (OP) is a progressive metabolic bone disease characterized by impaired microarchitecture, decreased strength, and dysregulated remodeling, leading to an increased risk of fractures. Among osteoporotic fractures, vertebral compression fractures (OVCF) are the most common can significantly impact patients' quality life. Growing evidence suggests that microRNAs (miRNAs) play crucial role in homeostasis regulating osteoblast differentiation, metabolism, remodeling processes. Notably, miR-147b-3p has been found be downregulated OVCF; however, its specific osteogenic regulation remains largely unknown. Therefore, further investigation warranted elucidate function underlying mechanism differentiation. The GSE93883 GSE74209 datasets were retrieved from Gene Expression Omnibus (GEO) database investigate involved osteogenesis. Differential expression NDUFA4 was assessed between healthy controls patients with using real-time quantitative PCR.To modulate levels MC3T3-E1 cells, both mimic inhibitor utilized. Cell viability evaluated via CCK-8 assay assess on cell proliferation. Real-time PCR Western blot analysis conducted quantify markers across different experimental groups. Alizarin red staining (ARS) employed examine effect mineralization capacity cells. In vivo experiments performed evaluate functional miR-147b-3p. Bioinformatics databases used predict potential target gene (NDUFA4), predictions validated dual luciferase reporter assay.To regulatory miR-147b-3p/NDUFA4 axis cells transfected overexpression plasmid mimic. phosphorylation PI3K AKT, order explore whether regulates differentiation through PI3K/AKT signaling pathway. Our results indicated significant downregulation concurrent upregulation (OVCF). A confirmed direct miR-147b-3p.To miR-147b-3p, vitro conducted.The findings revealed enhanced viability, protein expressions Alkaline Phosphatase (ALP) Runt-related Transcription Factor 2 (RUNX2), promoted as evidenced Red S staining. Conversely, treatment or for (pNDUFA4) produced opposite effects.Furthermore, regulate pathway.The (p-PI3K) AKT (p-AKT), whereas pNDUFA4 led their reduction. This study demonstrated plays promoting targeting modulating These provide new insights into molecular mechanisms progression

Language: Английский

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Advances in cell therapy for orthopedic diseases: bridging immune modulation and regeneration

Frontiers in Immunology, Journal Year: 2025, Volume and Issue: 16

Published: April 10, 2025

Orthopedic diseases pose significant challenges to public health due their high prevalence, debilitating effects, and limited treatment options. Additionally, orthopedic tumors, such as osteosarcoma, chondrosarcoma, Ewing sarcoma, further complicate the landscape. Current therapies, including pharmacological treatments joint replacement, address symptoms but fail promote true tissue regeneration. Cell-based which have shown successful clinical results in cancers other diseases, emerged a promising solution repair damaged tissues restore function tumors. This review discusses advances potential application of cell therapy for with particular focus on osteoarthritis, bone fractures, cartilage degeneration, We explore mesenchymal stromal cells (MSCs), chondrocyte transplantation, engineered immune induced pluripotent stem enhance regeneration by modulating response addressing inflammation. Ultimately, integration cutting-edge therapy, modulation, molecular targeting strategies could revolutionize providing hope patients seeking long-term solutions conditions.

Language: Английский

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Trends of mapping knowledge structure and themes of osteoporotic vertebral compression fracture: A bibliometric and visualized analysis

Asian Journal of Surgery, Journal Year: 2025, Volume and Issue: unknown

Published: April 1, 2025

Language: Английский

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From Bench to Bedside: Translating Cellular Rejuvenation Therapies into Clinical Applications

Cells, Journal Year: 2024, Volume and Issue: 13(24), P. 2052 - 2052

Published: Dec. 12, 2024

Cellular rejuvenation therapies represent a transformative frontier in addressing age-related decline and extending human health span. By targeting fundamental hallmarks of aging—such as genomic instability, epigenetic alterations, mitochondrial dysfunction, cellular senescence—these aim to restore youthful functionality cells tissues, offering new hope for treating degenerative diseases. Recent advancements have showcased range strategies, including reprogramming, senolytic interventions, restoration, stem cell-based approaches, gene-editing technologies like CRISPR. Each modality has demonstrated substantial potential preclinical models is now being cautiously explored early-stage clinical trials. However, translating these from the laboratory practice presents unique challenges: safety concerns, delivery precision, complex regulatory requirements, ethical considerations, high costs impede widespread adoption. This review examines current landscape rejuvenation, highlighting key advancements, risks, strategies needed overcome hurdles.

Language: Английский

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Gene expression and hormonal signaling in osteoporosis: from molecular mechanisms to clinical breakthroughs

Journal of Biomaterials Science Polymer Edition, Journal Year: 2024, Volume and Issue: unknown, P. 1 - 36

Published: Dec. 27, 2024

Osteoporosis is well noted to be a universal ailment that realization impaired bone mass and micro architectural deterioration thus enhancing the probability of fracture. Despite its high incidence, management remains highly demanding because multifactorial pathophysiology disease. This review highlights recent findings in osteoporosis particularly, gene expression hormonal control. Some newest approaches regarding subject are described, including single-cell RNA sequencing long non-coding RNAs. Also, reflects new on signaling estrogen parathyroid hormone; patient-specific due genetic variation. Potential biomarkers AI comprised as factors for improving ability anticipate manage fractures. These hold great potential drugs, combination therapies based future. Further studies cooperation scientists clinicians will help apply such novelties into practical uses sphere medicine order enhance treatment patients with osteoporosis.

Language: Английский

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Increased Risk of Osteoporotic Vertebral Compression Fractures Following Epidural Steroid Injections in Patients with Lumbar Degenerative Disease: A Retrospective Cohort Study

Journal of Clinical Medicine, Journal Year: 2024, Volume and Issue: 13(21), P. 6379 - 6379

Published: Oct. 24, 2024

: Lumbar degenerative disease is a common age-related condition, with epidural steroid injection (ESI) being widely employed conservative treatment approach. However, the potential effect of ESI on osteoporosis and fracture risk remains unclear. This study investigated osteoporotic vertebral compression fractures (OVCFs) in patients lumbar who underwent treatment.

Language: Английский

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Fixation Failure in Osteoporotic Bone: A Review of Complications and Outcomes

Indian Journal of Orthopaedics, Journal Year: 2024, Volume and Issue: 59(3), P. 389 - 404

Published: Dec. 21, 2024

Language: Английский

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