3D-Cultured MC3T3-E1-Derived Exosomes Promote Endothelial Cell Biological Function under the Effect of LIPUS DOI Creative Commons
Xiaohan Liu,

Rui Cheng,

Hongjuan Cao

et al.

Biomolecules, Journal Year: 2024, Volume and Issue: 14(9), P. 1154 - 1154

Published: Sept. 13, 2024

Porous Ti-6Al-4V scaffold materials can be used to heal massive bone defects because they provide space for vascularisation and formation. During new tissue development, rapid vascular ingrowth into is very important. Osteoblast-derived exosomes are capable of facilitating angiogenesis-osteogenesis coupling. Low-intensity pulsed ultrasound (LIPUS) a physical therapy modality widely utilised in the field regeneration has been proven enhance production functionality on two-dimensional surfaces. The impact LIPUS derived from osteoblasts cultured three dimensions remains elucidated. In this study, produced by porous under non-ultrasound stimulated conditions were co-cultured with endothelial cells. findings indicated that consistently stably taken up Compared group, group facilitated cell proliferation angiogenesis. After 24 h co-culture, migration ability cells was 17.30% higher relative group. may represent potentially viable strategy promote efficacy osteoblast-derived angiogenesis materials.

Language: Английский

Biomaterials for Guided Tissue Regeneration and Guided Bone Regeneration: A Review DOI Creative Commons
Nathália Dantas Duarte, Paula Buzo Frigério,

Gloria Estefania Amaya Chica

et al.

Dentistry Journal, Journal Year: 2025, Volume and Issue: 13(4), P. 179 - 179

Published: April 21, 2025

This review aims to provide an overview of the types membranes, bone substitutes, and mucosal substitutes used for GTR GBR briefly explores recent innovations tissue regeneration their future perspectives. Since this is a narrative review, no systematic search, meta-analysis, or statistical analysis was conducted. Using biomaterials provides reduction in postoperative morbidity, as it contributes less invasive clinical procedures, serving alternative autogenous grafts. Moreover, randomized trials (RCTs) reviews are essential evaluation new biomaterials. These studies more robust evidence help guide practice selection safer effective biomaterials, allowing personalization treatment protocols each patient.

Language: Английский

Citations

0
Effect of Hydrothermal Coatings of Magnesium AZ31 Alloy on Osteogenic Differentiation of hMSCs: From Gene to Protein Analysis DOI Open Access
Viviana Costa, Lavinia Raimondi, Simone Dario Scilabra

et al.

Materials, Journal Year: 2025, Volume and Issue: 18(6), P. 1254 - 1254

Published: March 12, 2025

An Mg-based alloy device manufactured via a superplastic forming process (Mg-AZ31+SPF) and coated using hydrothermal method (Mg AZ31+SPF+HT) was investigated as to increase mechanical osteointegration capability. The cell viability osteointegrative properties of alloy-derived Mg AZ31+SPF AZ31+SPF+HT extracts were regarding their effect on human mesenchymal stem cells (hMSCs) (maintained in basal (BM) osteogenic medium (OM)) after 7 14 days treatment. analyzed through metabolic activity double-strand DNA quantification, while the osteoinductive effects evaluated qRT-PCR, osteoimage, BioPlex investigations. Finally, preliminary liquid mass spectrometry analysis conducted secretome hMSCs. Biocompatibility revealed no toxic cells’ or proliferation during experimental period. A modulation observed osteoblast pre-commitment genes hMSCs treated with Mg-AZ31+SPF+HT OM, which supported by mineralization nodule analysis. investigation highlighted protein clusters involved extracellular exosomes, Hippo, lipid metabolism process. In conclusion, our results that can modulate canonical non-canonical vitro, suggesting possible application bone tissue engineering.

Language: Английский

Citations

0
Construction and Osteogenic Capacity Study of Engineered Exosome-based Nanoplatform Targeting Macrophages DOI Creative Commons
Wei Xiang,

Zhoujun Zhu,

Qisong Shang

et al.

Journal of Drug Delivery Science and Technology, Journal Year: 2025, Volume and Issue: unknown, P. 106912 - 106912

Published: April 1, 2025

Language: Английский

Citations

0
Exosomes: Key Factors in Ovarian Cancer Peritoneal Metastasis and Drug Resistance DOI Creative Commons
Ming Shao,

Yunran Gao,

Xiling Xu

et al.

Biomolecules, Journal Year: 2024, Volume and Issue: 14(9), P. 1099 - 1099

Published: Sept. 2, 2024

Ovarian cancer remains a leading cause of death among gynecological cancers, largely due to its propensity for peritoneal metastasis and the development drug resistance. This review concentrates on molecular underpinnings these two critical challenges. We delve into role exosomes, nano-sized vesicles integral cellular communication, in orchestrating complex interactions within tumor microenvironment that facilitate metastatic spread thwart therapeutic efforts. Specifically, we explore how exosomes drive by promoting epithelial–mesenchymal transition mesothelial cells, altering extracellular matrix, supporting angiogenesis, which collectively enable dissemination cells across cavity. Furthermore, dissect mechanisms contribute emergence resistance, including sequestration expulsion chemotherapeutic agents, horizontal transfer resistance genes, modulation DNA repair apoptotic pathways. By shedding light exosome-mediated processes, underscore potential exosomal pathways as novel targets, offering hope more effective interventions against ovarian cancer’s relentless progression.

Language: Английский

Citations

2
A Macroscopic Exploration of the Ideoscape on Exosomes for Bone Regeneration DOI Creative Commons
Maria Teresa Colangelo,

Marco Meleti,

Stefano Guizzardi

et al.

Osteology, Journal Year: 2024, Volume and Issue: 4(4), P. 159 - 178

Published: Oct. 8, 2024

Background: Exosomes, nanoscale extracellular vesicles, play a crucial role in tissue physiology and regeneration. This study uses infometric techniques to explore the structure of exosome-based bone regeneration research. Methods: We applied BERTopic, an advanced topic modeling algorithm, comprehensive corpus scientific literature on exosomes regeneration, identifying key themes such as stem cell studies, healing, regenerative applications, with orthopedics dentistry emerging dominant subfields. To further investigate ‘ideoscape’, i.e., conceptual landscape that maps how ideas, methods, are interconnected across field, we extracted significant concepts from abstracts using GPT 3.5 turbo created knowledge graphs. Results: Our analysis revealed rapid growth field dental which has outpaced other topics by twofold. highlighted central periodontal cells their cellular processes—proliferation, migration, differentiation—along clinical applications. approach provided clear visualization field’s intellectual structure, showing interconnected. findings offer view evolving trends revealing not only most active research areas but also gaps opportunities for investigation. Conclusions: exemplifies utility combining graph creation map trends, offering flexible largely automated tool researchers vast bodies guide future directions.

Language: Английский

Citations

1
Progress of Exosomal LncRNAs in Pancreatic Cancer DOI Open Access

Chengyan Wei,

Chunwei Zhang, Y. Z. Zhou

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(16), P. 8665 - 8665

Published: Aug. 8, 2024

Pancreatic cancer is a prevalent malignant tumor with rising medication resistance and mortality. Due to dearth of specific trustworthy biomarkers therapeutic targets, pancreatic early detection treatment are still not at their best. Exosomal LncRNAs have been found be plentiful persistent within exosomes, they capable functioning whether the exosomes traveling close or distant cells. Furthermore, increasing evidence suggests that exosomal LncRNA, identified as an oncogene suppressor-control growth, metastasis, susceptibility chemotherapy radiation therapy. Promising prospects for both antitumor targets diagnostic LncRNAs. The primary features LncRNAs, biological roles in onset progression cancer, potential molecular markers outlined this review.

Language: Английский

Citations

1
3D-Cultured MC3T3-E1-Derived Exosomes Promote Endothelial Cell Biological Function under the Effect of LIPUS DOI Creative Commons
Xiaohan Liu,

Rui Cheng,

Hongjuan Cao

et al.

Biomolecules, Journal Year: 2024, Volume and Issue: 14(9), P. 1154 - 1154

Published: Sept. 13, 2024

Porous Ti-6Al-4V scaffold materials can be used to heal massive bone defects because they provide space for vascularisation and formation. During new tissue development, rapid vascular ingrowth into is very important. Osteoblast-derived exosomes are capable of facilitating angiogenesis-osteogenesis coupling. Low-intensity pulsed ultrasound (LIPUS) a physical therapy modality widely utilised in the field regeneration has been proven enhance production functionality on two-dimensional surfaces. The impact LIPUS derived from osteoblasts cultured three dimensions remains elucidated. In this study, produced by porous under non-ultrasound stimulated conditions were co-cultured with endothelial cells. findings indicated that consistently stably taken up Compared group, group facilitated cell proliferation angiogenesis. After 24 h co-culture, migration ability cells was 17.30% higher relative group. may represent potentially viable strategy promote efficacy osteoblast-derived angiogenesis materials.

Language: Английский

Citations

1