Frontiers in Neuroscience,
Journal Year:
2024,
Volume and Issue:
18
Published: Dec. 20, 2024
Postoperative
cognitive
dysfunction
(POCD)
is
a
prevalent
complication
that
significantly
affects
the
quality
of
life.
Notably,
patients
who
have
experienced
ischemic
stroke
are
at
an
increased
risk
developing
POCD.
Exploring
underlying
mechanisms
POCD
crucial
for
its
management.
Numerous
studies
established
neuroinflammation
as
independent
factor
in
pathogenesis,
with
TREM2
emerging
key
neuroprotective
modulates
neuroinflammatory
responses
through
PI3K/Akt
signaling
pathway.
In
this
study,
we
aimed
to
investigate
effect
on
mouse
model
stroke,
focus
involving
and
Our
findings
indicated
mice
exhibited
severe
impairment
after
surgical
trauma.
However,
observed
enriched
environment
(EE)
could
ameliorate
by
upregulating
microglia
expression
hippocampus
suppressing
neuroinflammation.
Additionally,
PI3K/AKT
pathway
was
activated
hippocampal
tissue
housed
EE.
Importantly,
beneficial
anti-inflammatory
effects
EE
were
abolished
when
knocked
down,
underscoring
essential
role
mediating
function
general,
our
study
has
confirmed
potential
molecular
mechanism
led
occurrence
individuals
provided
new
strategies
treat
Frontiers in Genetics,
Journal Year:
2025,
Volume and Issue:
16
Published: March 18, 2025
Cotton
is
a
vital
fiber
crop
for
the
global
textile
industry,
but
rising
temperatures
due
to
climate
change
threaten
its
growth,
quality
and
yields.
Heat
stress
disrupts
key
physiological
biochemical
processes,
affecting
carbohydrate
metabolism,
hormone
signaling,
calcium
gene
regulation
expression.
This
review
article
explores
cotton's
defense
mechanism
against
heat
stress,
including
epigenetic
regulations
transgenic
approaches,
with
focus
on
genome
editing
tools.
Given
limitations
of
traditional
breeding,
advanced
omics
technologies
such
as
GWAS,
transcriptomics,
proteomics,
ionomics,
metabolomics,
phenomics
CRISPR-Cas9
offer
promising
solutions
developing
heat-resistant
cotton
varieties.
highlights
need
innovative
strategies
ensure
sustainable
production
under
change.
International Journal of General Medicine,
Journal Year:
2025,
Volume and Issue:
Volume 18, P. 103 - 111
Published: Jan. 1, 2025
This
study
aimed
to
investigate
the
clinical
significance
of
heat
shock
protein
60
(HSP60)
expression
in
ovarian
cancer
and
evaluate
its
correlation
with
patient
survival
outcomes.
A
total
260
patients
diagnosed
between
2017
2019
were
enrolled.
Immunohistochemistry
was
performed
assess
HSP60
tumor
tissues.
Patients
categorized
into
high-
or
low-HSP60
groups
based
on
immunohistochemical
staining
intensity.
The
status
clinicopathological
features
analyzed.
Kaplan-Meier
curves
Cox
regression
models
utilized
overall
disease-free
survival.
significantly
higher
tissues
compared
normal
High
associated
larger
size,
advanced
FIGO
stage,
increased
lymph
node
metastasis.
high
exhibited
shorter
than
those
low
expression.
Multivariate
analysis
identified
as
an
independent
prognostic
factor
for
both
is
poor
prognosis
aggressive
characteristics
cancer.
may
serve
a
valuable
biomarker
potential
therapeutic
target.
Further
randomized
trials
are
warranted
explore
role
progression
treatment
strategies.
The FASEB Journal,
Journal Year:
2025,
Volume and Issue:
39(2)
Published: Jan. 24, 2025
Abstract
Gestational
Diabetes
Mellitus
(GDM)
is
the
most
frequent
complication
during
pregnancy.
Pharmacological
interventions,
such
as
peptide
drugs
that
focused
on
improving
insulin
sensitivity
might
be
promising
in
prevention
and
treatment
of
GDM.
In
this
study,
we
aimed
to
investigate
role
mechanism
a
novel
peptide,
named
AGDMP1
(Anti‐GDM
1),
which
previously
identified
lower
serum
GDM
patients
using
mass
spectrometry,
adipose
resistance
We
found
had
high
affinity
for
tissues
vivo
.
markedly
improved
glucose
homeostasis
mice.
This
was
associated
with
reduced
inflammation
upregulated
AKT/GLUT4
signaling
pathway
white
tissue.
vitro
,
could
increase
uptake
adipocytes
by
activating
IRS‐1/AKT/GLUT4
under
basal,
insulin‐stimulated,
insulin‐resistant
conditions,
respectively.
Mechanistically,
bind
HSP60
dampen
its
effects
AKT
pro‐inflammatory
response,
reversed
present
recombinant
protein.
ameliorated
targeting
pathway.
These
data
supported
therapeutic
potential
pathologies.
Insects,
Journal Year:
2025,
Volume and Issue:
16(2), P. 127 - 127
Published: Jan. 28, 2025
Heat
shock
proteins
(HSPs)
are
crucial
molecular
chaperones
that
help
organisms
maintain
protein
stability
under
stress
conditions.
As
a
major
stored-product
pest,
Rhyzopertha
dominica
(Fabricius)
faces
distinct
stresses
compared
to
field
insects,
primarily
due
the
specific
pest
control
methods
applied
during
grain
storage.
In
this
study,
total
of
53
HSP
genes
from
five
gene
families
(HSP90,
HSP70,
HSP60,
sHSP,
and
DnaJ)
were
identified
characterized
using
bioinformatics
methods.
Among
them,
DnaJ
was
largest
most
diverse
family
in
R.
dominica.
Transcriptome
sequencing
RT-qPCR
then
used
evaluate
expression
patterns
four
storage-related
stresses,
following
series
bioassays.
Extreme
high
temperature
strongest
inducer
expression,
with
12
showing
over
10-fold
increase.
Controlled
nitrogen
atmosphere
also
led
considerable
upregulation
genes,
especially
HSP70
family.
contrast,
phosphine
fumigation
K-Obiol
protectant
caused
very
limited
induction
which
might
have
been
less
severe
damage
by
chemical
physical
stresses.
Our
study
provides
theoretical
basis
for
further
research
on
functions
Cells,
Journal Year:
2025,
Volume and Issue:
14(3), P. 204 - 204
Published: Jan. 30, 2025
The
epichaperome,
a
dynamic
and
integrated
network
of
chaperone
proteins,
extends
its
roles
beyond
basic
protein
folding
to
stabilization
intracellular
signal
transduction
orchestrating
multitude
cellular
processes
critical
for
tumor
survival.
In
this
review,
we
explore
the
multifaceted
delving
into
diverse
locations,
factors
that
modulate
formation
function,
liquid–liquid
phase
separation,
key
signaling
crosstalk
pathways
it
regulates,
including
metabolism
transduction.
We
further
highlight
techniques
isolating
identifying
epichaperome
networks,
pitfalls,
opportunities.
Further,
review
profound
implications
cancer
treatment
therapy
design,
underscoring
need
strategic
engineering
hinges
on
comprehensive
insight
structure
workings
across
heterogeneous
cell
subpopulations
in
milieu.
By
presenting
holistic
view
epichaperome’s
functions
mechanisms,
aim
underscore
potential
as
target
novel
anti-cancer
strategies,
revealing
is
not
merely
piece
machinery
but
mastermind
facilitates
malignant
phenotype.
Children,
Journal Year:
2025,
Volume and Issue:
12(2), P. 205 - 205
Published: Feb. 8, 2025
Background:
The
rising
rates
of
gallstones
and
cholecystectomy
in
pediatric
populations
underscore
the
increasing
concern
regarding
chronic
cholecystitis.
However,
morphopathogenesis
calculous
cholecystitis
is
still
not
well
understood.
This
study
aimed
to
determine
expression
distribution
immunomodulatory
factors
interleukin-12
(IL-12),
interleukin-13
(IL-13),
interleukin-1β
(IL-1β),
sonic
hedgehog
protein
(SHH),
nuclear
factor
NF-kappa-B
p65
subunit
(NFkBp65),
heat
shock
60
(HSP60)
gallbladder
walls
patients
with
Methods:
In
total,
11
samples
were
collected
from
during
cholecystectomy,
while
5
healthy
served
as
controls.
IL-12,
IL-13,
IL-1β,
SHH,
NFkBp65,
HSP60
detected
by
immunohistochemistry.
number
positive
structures
wall
epithelium,
vasculature,
inflammatory
infiltrate
was
assessed
semi-quantitatively
microscopy.
A
Mann–Whitney
U
test
Spearman’s
rank-order
correlation
coefficient
calculated.
Results:
Statistically
significant
differences
observed
between
patient
control
NFkBp65
blood
vessels.
IL-1β
stronger
epithelium
controls,
other
markers
more
prominent
samples.
Conclusions:
An
increased
cells
tissue
suggests
a
role
these
driving
immune
modulation
sustaining
inflammation
noticeable
SHH
indicates
its
part
regeneration
repair
processes,
modulating
vascular
responses
correlations
studied
highlight
importance
their
coordinated
interaction
intricate
crosstalk
Neurobiology of Disease,
Journal Year:
2025,
Volume and Issue:
206, P. 106838 - 106838
Published: Feb. 10, 2025
Despite
the
availability
of
various
antiepileptic
treatments,
approximately
30
%
epilepsy
patients
remain
refractory
to
conventional
therapies,
underscoring
need
for
neuroprotective
strategies.
This
study
investigates
role
GrpEL1
in
modulating
mitochondrial
unfolded
protein
response
(UPRmt)
and
its
potential
protective
effects
on
hippocampal
neurons
following
experimental
status
epilepticus
(SE).
The
were
assessed
vivo
using
a
Lithium-pilocarpine
rat
model
SE
vitro
with
glutamate-treated
HT22
cells.
Protein
expression
interactions
analyzed
by
Western
blot,
immunofluorescence,
co-immunoprecipitation.
Neuronal
survival
was
evaluated
through
Nissl
staining.
Mitochondrial
function
aggresome
formation,
membrane
(MMP)
assays,
oxygen
consumption
rate
(OCR)
measurements,
behavioral
assessments
Morris
water
maze.
In
model,
mtHSP70
levels
significantly
upregulated
mitochondria,
while
remained
relatively
stable.
Overexpression
led
reduction
neuronal
damage
improved
functional
recovery
post-SE.
vitro,
overexpression
enhanced
GrpEL1-mtHSP70
interaction,
reduced
accumulation
misfolded
proteins,
decreased
apoptosis.
Furthermore,
mitigated
dysfunction
preserving
MMP
improving
bioenergetics,
as
evidenced
OCR.
plays
crucial
maintaining
proteostasis
mitigating
injury
regulating
UPRmt.
These
findings
suggest
that
may
represent
promising
target
therapeutic
intervention
protect
against
seizure-induced
neurodegeneration.
