Tetrahedral Framework Nucleic Acid‐Based Delivery of DJ‐1‐saRNA Prevent Retinal Ischaemia–Reperfusion Injury via Inhibiting Ferroptosis

Cell Proliferation, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 20, 2025

ABSTRACT Retinal ischaemia/reperfusion injury (RI/RI) is the primary pathophysiological mechanism underlying retinal ischaemic diseases, potentially resulting in significant and irreversible visual impairment. Currently, there are no effective treatments available for RI/RI, oxidative stress a critical factor that contributes to associated damage. DJ‐1, an important endogenous antioxidant, has been proposed as promising therapeutic agent RI/RI owing its potential overexpression. In this study, tetrahedral frame nucleic acids (tFNAs) were utilised delivery vehicle DJ‐1 small activating RNA (saRNA), synthesis of novel nanocomposite (tFNAs‐DJ‐1‐saRNA). vitro experiments demonstrated tFNAs effectively delivered DJ‐1‐saRNA R28 cells, thus exerting repair effect on injury. vivo investigations revealed intravitreal injection tFNAs‐DJ‐1‐saRNA facilitated gene expression mitigated atrophy induced by RI/RI. Mechanistically, activated xCT/GPX4 pathway, thereby inhibiting ferroptosis, reducing ganglion cell damage protecting tissue. conclusion, study complex can ameliorate suggesting treatment diseases.

Language: Английский

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The causal impact of gut microbiota and metabolites on myopia and pathological myopia: a mediation Mendelian randomization study

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: April 15, 2025

Myopia, a major cause of irreversible visual impairment globally, is projected to affect about 25% the world's population by 2025. Myopia progresses through childhood and adolescence, necessitating frequent prescription updates. While genetic environmental factors are well-established contributors myopia, emerging evidence suggests significant role gut microbiota (GM) in its development, mainly metabolic interactions. This study utilized Mendelian Randomization (MR) approach investigate causal relationships between GM, metabolites, myopia pathological (PM) progression. Using variants as instrumental variables, we analyzed data from extensive genome-wide association studies (GWAS) assess impacts 473 GM taxa 233 metabolites on risks. Our MR analysis identified specific with relationship PM. Notably, lipid were found mediate effects suggesting biochemical pathway that could influence ocular development We also observed mediation effects, indicating might serve therapeutic targets modulate The findings highlight potential novel for preventing or managing myopia. underscores importance further research into gut-metabolite-eye axis develop targeted interventions based modifying diet, probiotics, other means. Future should aim elucidate involved their roles health, potentially offering new avenues treatment.

Language: Английский

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L-shaped association between oxidative balance score and vision-related functional burden in adults in the United States, NHANES 2005–2008

Frontiers in Nutrition, Journal Year: 2025, Volume and Issue: 12

Published: May 2, 2025

Objective This study aims to investigate the relationship between oxidative balance score (OBS) and vision-related functional burden (VRFB) in US adults. Methods The utilized data from two consecutive cycles of National Health Nutrition Examination Survey (NHANES) 2005 2008. A multivariate weighted logistic regression model was employed explore OBS VRFB general population subgroups, while interaction effects were tested with a likelihood test. Restricted cubic spline assess nonlinear association VRFB. Sensitivity analyses performed test robustness results. Results total 6,682 participants aged 20 years older included NHANES database. negative observed fully adjusted model, an odds ratio (OR) 0.968 [95% confidence interval (CI): 0.949–0.987]. Compared lowest quartile OBS, second (OR: 0.669, 95%CI: 0.486–0.922), third 0.589, 0.403–0.859), highest 0.554, 0.359–0.855) quartiles associated reduced risk An L-shaped displayed ( P for = 0.016). Notably, when analysis stratified by employment status, significant < 0.05). protective effect more pronounced among unemployed. results found be robust sensitivity analyses. Conclusion U.S. adults older. novel finding suggests that maintaining favorable through modifiable dietary lifestyle (such as increased physical activity, smoking cessation, alcohol consumption) may help protect vision. We also stronger provides new insights into prevention vision loss, highlighting importance not only factors but considering different such Further longitudinal studies are required further validate these observations elucidate underlying mechanisms, particularly elucidating causal relationships variables, controlling confounding factors, examining development visual function.

Language: Английский

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From Nature to Treatment: The Impact of Pterostilbene on Mitigating Retinal Ischemia–Reperfusion Damage by Reducing Oxidative Stress, Inflammation, and Apoptosis

Life, Journal Year: 2024, Volume and Issue: 14(9), P. 1148 - 1148

Published: Sept. 11, 2024

Retinal ischemia-reperfusion (I/R) injury is a critical pathogenic mechanism in various eye diseases, and an effective therapeutic strategy remains unresolved. Natural derivatives have recently reemerged; therefore, our present study, we examined the potential effects of stilbenoid that chemically related to resveratrol. Pterostilbene, recognized for its anti-inflammatory, anti-carcinogenic, anti-diabetic, neuroprotective properties, counteracts oxidative stress during I/R through mechanisms. This study explored pterostilbene as retinoprotective agent. Male Sprague Dawley rats underwent retinal one-week reperfusion were treated with either vehicle or pterostilbene. After this functional electroretinographical (ERG) measurement, Western blot histological analyses performed. Pterostilbene treatment significantly improved function, evidenced by increased b-wave amplitude on ERG. Histological studies showed reduced thinning preserved structure pterostilbene-treated groups. Moreover, analysis revealed decreased expression glial fibrillary acidic protein (GFAP) heat shock 70 (HSP70), indicating activation cellular stress. Additionally, pro-apoptotic inflammatory markers, poly(ADP-ribose) polymerase 1 (PARP1) nuclear factor kappa B (NFκB) was group. These findings suggest offers protective retina diminishing stress, inflammation, apoptosis, thus preserving function following injury. underscores pterostilbene's agent treating ischemic disorders.

Language: Английский

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Aging Vasculature in the Choroid and Retina

Elsevier eBooks, Journal Year: 2024, Volume and Issue: unknown

Published: Jan. 1, 2024

Language: Английский

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