Biochemical evaluation of molecular parts for flavonoid production using plant synthetic biology DOI Creative Commons
Hyo Lee,

Saet Buyl Lee,

Sangkyu Park

et al.

Frontiers in Plant Science, Journal Year: 2025, Volume and Issue: 16

Published: April 15, 2025

Among organisms on Earth, plants have the unique ability to produce a wide variety of biomolecules using soil nutrients, air, and solar energy. Therefore, are regarded as most productive cost-efficient bioreactors among living organisms. Flavonoids, major group secondary metabolites exclusively produced in plants, play crucial roles plant physiology various effects human health. Flavonoids used diverse industries such pharmaceutical, nutraceutical, cosmetics industries. These compounds typically extracted from specific that naturally large amounts target flavonoid for commercial production. However, with increasing demand flavonoids, efforts been made enhance production synthetic biology sustainable microbes or plants. Synthetic has utilized metabolic engineering reconstitute biosynthetic pathways flavonoids at whole-pathway level, thereby enhancing For efficient biology, first all, optimized molecular parts enzymes must be identified selected. The best modules precursors final can then constructed these parts. In this review, we summarize enzyme kinetics natural engineered derived different species provide insight into selection parts, design devices, reconstitution based performance biology.

Language: Английский

Bioreduction of 4′-Hydroxychalcone in Deep Eutectic Solvents: Optimization and Efficacy with Various Yeast Strains DOI Open Access
Paweł Chlipała, Tomasz Janeczko, Marcelina Mazur

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(13), P. 7152 - 7152

Published: June 28, 2024

4′-dihydrochalcones are secondary metabolites isolated from many medicinal plants and the resin known as ‘dragon’s blood’. Due to their biological potential, our research objective was determine possibilities of using biocatalysis processes carried out in deep eutectic solvents (DESs) obtain a model compound. The were culture yeast Yarrowia lipolytica KCh 71 also cultures strains genera Rhodotorula Debaryomyces. Based on experiments out, an optimum process temperature 35 °C chosen, most suitable DES contained glycerol hydrogen bond donor (HBD). For medium with 30% water content (DES 11), conversion observed after 24 h exceeded 70%, while increasing amount 50% resulted similar level just 1 h. A fivefold increase added substrate reduction conversion, which reached 30.3%. Of other tested, marina 77 rubra 4 proved be good biocatalysts for bioreduction process. these strains, 95.4% 95.1%, respectively. These findings highlight potential biocatalyst selective α,β-unsaturated ketones possibility DESs reaction this

Language: Английский

Citations

5
The Gmbhlh13-Gmchs7 Module Positively Regulates Isoflavones Accumulation in Soybean (Glycine Max. L) DOI
Tianyi Liu, Fan Yan, Yajing Liu

et al.

Published: Jan. 1, 2025

Language: Английский

Citations

0
Evolutionary landscape of plant chalcone isomerase-fold gene families DOI Creative Commons

Kaiyong Luo,

Shiping Wang, Ling Yang

et al.

Frontiers in Plant Science, Journal Year: 2025, Volume and Issue: 16

Published: March 28, 2025

Flavonoids are crucial for plant survival and adaptive evolution, chalcone isomerase (CHI) genes serve as key rate-limiting gene in the flavonoid biosynthesis pathway. It is important evolution to comprehensively study diversity of CHI families. However, families many lineages remain elusive. This systematically identified from 259 species including algae, bryophytes, ferns, gymnosperms, angiosperms. A total 1,738 family members were discovered. We analyzed diversity, distribution trajectory, driving forces duplication during lineages. The present first identify potential type II IV extant liverwort model Marchantia polymorpha. pattern across kingdom reveals that origin can be traced back last common ancestor bryophytes vascular plants, III may represent ancestral form family. identification conserved motifs showed significant differences motif among different types. was found drivers varied lineages: dispersed duplications (DSD) predominant algae whole-genome (WGD) main driver basal angiosperms monocots, while tandem (TD) predominating eudicots. Structural clustering analysis demonstrated 3-layer sandwich structure CHI-fold proteins remained central region, repeated loss N-terminal sequences contributed structural diversity. provides a deeper understanding lays theoretical foundation further studies their function new functional genes.

Language: Английский

Citations

0
Functional analysis of type II chalcone isomerase ( CHI ) genes in regulating soybean ( Glycine max L .) nodule formation DOI Creative Commons
Xinyue Wang,

Jingwen Li,

Yu Zhou

et al.

GM crops & food, Journal Year: 2025, Volume and Issue: 16(1), P. 305 - 317

Published: March 31, 2025

Biological nitrogen fixation (BNF) is the most cost-effective and environmentally benign method for fertilization. Isoflavones are important signaling factors BNF in leguminous plants. Whether chalcone isomerase (CHI), key enzyme gene flavonoid synthesis pathway, contributes to soybean (Glycine max) nodulation has not yet been fully clarified. In present study, we identified functions of three types GmCHI using a hairy root system. The results showed that GmCHI1A GmCHI1B1 positively increased while GmCHI1B2 did not, with having greater effect than GmCHI1B1. Meanwhile, daidzein genistein contents were significantly composite plants overexpressing reduced plants, thus interfering GmCHI1A. However, overexpression content glycitein but daidzein, implied homologous genes exhibit functional differentiation. These provide reference subsequent studies on improving soybeans providing improvement new varieties.

Language: Английский

Citations

0
Metabolic and transcriptomic responses of <i>Taxus mairei</i> to nano-pollutants: insights into AgNPs and PsNPs impact DOI Creative Commons
Zhijin Wang, Pengcheng Zhang, Wei Yao

et al.

Medicinal Plant Biology, Journal Year: 2025, Volume and Issue: 4(1), P. 0 - 0

Published: Jan. 1, 2025

Language: Английский

Citations

0
Biochemical evaluation of molecular parts for flavonoid production using plant synthetic biology DOI Creative Commons
Hyo Lee,

Saet Buyl Lee,

Sangkyu Park

et al.

Frontiers in Plant Science, Journal Year: 2025, Volume and Issue: 16

Published: April 15, 2025

Among organisms on Earth, plants have the unique ability to produce a wide variety of biomolecules using soil nutrients, air, and solar energy. Therefore, are regarded as most productive cost-efficient bioreactors among living organisms. Flavonoids, major group secondary metabolites exclusively produced in plants, play crucial roles plant physiology various effects human health. Flavonoids used diverse industries such pharmaceutical, nutraceutical, cosmetics industries. These compounds typically extracted from specific that naturally large amounts target flavonoid for commercial production. However, with increasing demand flavonoids, efforts been made enhance production synthetic biology sustainable microbes or plants. Synthetic has utilized metabolic engineering reconstitute biosynthetic pathways flavonoids at whole-pathway level, thereby enhancing For efficient biology, first all, optimized molecular parts enzymes must be identified selected. The best modules precursors final can then constructed these parts. In this review, we summarize enzyme kinetics natural engineered derived different species provide insight into selection parts, design devices, reconstitution based performance biology.

Language: Английский

Citations

0