Involvement of miR775 in the Post-Transcriptional Regulation of Fructose-1,6-Bisphosphate Aldolase in Maize (Zea mays L.) Leaves Under Hypoxia

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(3), P. 865 - 865

Published: Jan. 21, 2025

Different types of microRNA participate in the post-transcriptional regulation target genes. The content several hypoxia-dependent miRNAs plant cells, including miR775, increases conditions oxygen deficiency. Electrophoretic studies total RNA samples from leaves flooded seedlings maize (Zea mays L.) revealed presence two interfering complexes with miR775 at 12 h hypoxic incubation. A nucleotide sequence analysis a sample containing complex mRNA showed high degree homology ICL/PEPM_KPHMT lyase family domain. It corresponded to fragment fructose-1,6-bisphosphate aldolase mRNA. By real-time PCR, we established dynamics transcripts isoenzyme genes under hypoxia leaves. decrease transcriptional activity 1 gene (Aldo1) correlated leaf cells. fraction extracellular vesicles sedimented 100,000× g, was enriched miR775. accumulation 2 (Aldo2) indicates its participation maintaining glycolysis when Aldo1 expression is inhibited. We conclude that an increase free and suppression represents important mechanism developing adaptive reaction cellular metabolism response hypoxia.

Language: Английский

The Role of DNA Methyltransferases of Different Families in Changing the Methyl Status of DNA in Corn Leaves under Irradiation with Light of Different Wavelengths

Russian Journal of Plant Physiology, Journal Year: 2025, Volume and Issue: 72(1)

Published: Feb. 1, 2025

Language: Английский