Potassium Current Signature of Neuronal/Glial Progenitors in Amniotic Fluid Stem Cells

Cells, Journal Year: 2025, Volume and Issue: 14(1), P. 50 - 50

Published: Jan. 4, 2025

Amniotic fluid is a complex and dynamic biological matrix that surrounds the fetus during pregnancy. From this fluid, possible to isolate various cell types with particular interest directed towards stem cells (AF-SCs). These are highly appealing due their numerous potential applications in field of regenerative medicine for tissues organs as well treating conditions such traumatic or ischemic injuries nervous system, myocardial infarction, cancer. AF-SCs, when subcultured presence basic Fibroblast Growth Factor (bFGF), have been shown survive migrate transplanted into striatum rat brain, exhibiting behavior characteristics neuronal/glial progenitor cells. In work, we performed an electrophysiological characterization ascertain propensity AF-SCs differentiate glial neuronal by bFGF. By using patch clamp technique characterized fibroblast-like morphology display barium-sensitive inward-rectifying potassium current (Kir) calcium-activated currents (KCa). The calcium dynamics histamine, marker undifferentiated neural progenitors, was further studied. Histamine promoted intracellular increase Fura-2 recording activation similar temporal profile AF-SC. data presented paper ultimately confirm expression Kir KCa currents, also showing regulation endogenous stimuli histamine latter.

Language: Английский

Stable expression of SARS-CoV-2 envelope viroporin promotes intracellular calcium depletion in human cells: relevance for endoplasmic reticulum stress, cell proliferation, pluripotency and lineage differentiation

Cell Calcium, Journal Year: 2025, Volume and Issue: 128, P. 103032 - 103032

Published: April 18, 2025

Language: Английский