Impact of Long-Term Cannabidiol (CBD) Treatment on Mouse Kidney Transcriptome DOI Open Access

M Rokicki,

Jakub Żurowski,

Sebastian Sawicki

et al.

Genes, Journal Year: 2024, Volume and Issue: 15(12), P. 1640 - 1640

Published: Dec. 21, 2024

Background: Cannabidiol, which is one of the main cannabinoids present in Cannabis sativa plants, has been shown to have therapeutic properties, including anti-inflammatory and antioxidant effects that may be useful for treatment various kidney conditions. Objectives: This article investigates effect long-term cannabidiol (CBD) on changes renal transcriptome a mouse model. The hypothesis was systematic CBD would affect gene expression associated with those processes kidney. Methods: study conducted male C57BL/6J mice. Mice experimental groups received daily intraperitoneal injections at doses 10 mg/kg or 20 body weight (b.w.) 28 days. After experiment, tissues were collected, RNA isolated, RNA-Seq sequencing performed. Results: results show CBD’s expression, regulation genes related circadian rhythm (e.g., Ciart, Nr1d1, Nr1d2, Per2, Per3), glucocorticoid receptor function Cyp1b1, Ddit4, Foxo3, Gjb2, Pck1), lipid metabolism Cyp2d22, Cyp2d9, Decr2 Hacl1, Sphk1), inflammatory response Cxcr4 Ccl28). Conclusions: obtained suggest beneficial purposes treating disease, its should further analyzed clinical trials.

Language: Английский

Unveiling the Complexities of Medications, Substance Abuse, and Plants for Recreational and Narcotic Purposes: An In-Depth Analysis DOI Creative Commons

Iasmina-Alexandra Predescu,

Alex-Robert Jîjie,

Dalia Pătrașcu

et al.

Pharmacy, Journal Year: 2025, Volume and Issue: 13(1), P. 7 - 7

Published: Jan. 22, 2025

The complexities surrounding the use of medications, substance abuse, and recreational plants are multifaceted warrant a comprehensive examination. This review highlights consumption chemical substances in excess or for non-medical purposes, obtained through legal prescriptions, over-the-counter purchases, illicit means, with an emphasis on predictive role stressors individual-level variables development disorders, as well influence regulatory environment patterns consumption. Additionally, alarming escalation mortality rate associated drug opioid overdoses is also underscored. prescription medications can lead to significant health risks, particularly when combined other substances; therefore, need interventions preventive measures address abuse among various populations imperative. Furthermore, novel insights addiction, exploring neurobiological mechanisms underlying discussing treatment approaches elucidated. Advancements technology detecting highlighted, displaying innovative tools more effective identification monitoring. In conclusion, reveal landscape marked by overlapping motivations implications. distinction between medical critical understanding user behavior addressing public concerns.

Language: Английский

Citations

0
FAAH Modulators from Natural Sources: A Collection of New Potential Drugs DOI Creative Commons

Catalin Nicoara,

Filomena Fezza, Mauro Maccarrone

et al.

Cells, Journal Year: 2025, Volume and Issue: 14(7), P. 551 - 551

Published: April 5, 2025

The endocannabinoid system (ECS) plays a crucial role in maintaining homeostasis by regulating immune response, energy metabolism, cognitive functions, and neuronal activity. It consists of endocannabinoids (eCBs), cannabinoid receptors (CBRs), enzymes involved eCB biosynthesis degradation. Increasing evidence highlights the involvement ECS under several pathological conditions, making it promising therapeutic target. Recent research efforts have focused on modulating endogenous levels, particularly through inhibition fatty acid amide hydrolase (FAAH), main catabolic enzyme major anandamide. Natural substances, including plant extracts purified compounds, can inhibit FAAH represent area pharmacological research. inhibitors are attractive due to their potentially lower toxicity compared synthetic them safer candidates for applications. Phytocannabinoids, flavonoids, flavolignans been shown efficiently FAAH. structural diversity bioactivity these natural substances provide valuable alternative inhibitors, may open new avenues developing innovative tools.

Language: Английский

Citations

0
Exploring Membrane Cholesterol Binding to the CB1 Receptor: A Computational Perspective DOI

Manuela J. Vanegas,

Sara Gómez, Chiara Cappelli

et al.

The Journal of Physical Chemistry B, Journal Year: 2025, Volume and Issue: unknown

Published: April 23, 2025

Cholesterol (CHOL) is a potential allosteric modulator of the CB1 receptor. In this work, we use atomistic molecular dynamics simulations to study how CHOL interacts with and identify its binding sites (BS) residence times on specific receptor zones. Our results evince minimal changes in conformational secondary structure due CHOL. We report five BSs, three which coincide previously described interaction regions (BS1, BS2, BS3), while BS4 BS5 are proposed as new BSs. Quantum descriptors bonding such Natural Bond Orbitals (NBO), Theory Atoms Molecules (QTAIM), Noncovalent Interactions (NCI) analyses employed characterize CHOL-BS interactions. The show an exponential correlation between strength interactions (mainly hydrogen bonds hydrophobic contacts) time at Although other approaches exist high-affinity protein sites, our methodology integrates classical quantum descriptions better BSs predict ligand CB1, distinguishing persistent from transitory contacts. Since has been suggested endogenous ligand, flexible strategy allows studying that stabilize could be extended cannabinoid binding, contribute designing improved ligands.

Language: Английский

Citations

0
Efficiency and safety of cannabinoid medical use: an analysis of discussions and observed trends on Instagram DOI Creative Commons
Olena Litvinova, Bikash Baral, Thomas Wochele‐Thoma

et al.

Frontiers in Public Health, Journal Year: 2024, Volume and Issue: 12

Published: Dec. 4, 2024

Cannabis and its derivatives show encouraging therapeutic effects in the treatment of various diseases. However, further studies are needed to better assess their efficacy safety. A promising base for research field medicine additional pharmacovigilance is social networks, which experience knowledge exchanged between researchers, doctors, patients, as well information about potential risks benefits using drugs medical purposes disseminated. The aim this study was investigate reported efficiency safety use cannabinoids patients posts on media Instagram analyze observed trends.

Language: Английский

Citations

1
Role of gut dysbiosis in drug-resistant epilepsy: Pathogenesis and available therapeutic strategies DOI
Nidhi Khedpande,

Kalyani Barve

Brain Research, Journal Year: 2024, Volume and Issue: 1850, P. 149385 - 149385

Published: Dec. 5, 2024

Language: Английский

Citations

0
Impact of Long-Term Cannabidiol (CBD) Treatment on Mouse Kidney Transcriptome DOI Open Access

M Rokicki,

Jakub Żurowski,

Sebastian Sawicki

et al.

Genes, Journal Year: 2024, Volume and Issue: 15(12), P. 1640 - 1640

Published: Dec. 21, 2024

Background: Cannabidiol, which is one of the main cannabinoids present in Cannabis sativa plants, has been shown to have therapeutic properties, including anti-inflammatory and antioxidant effects that may be useful for treatment various kidney conditions. Objectives: This article investigates effect long-term cannabidiol (CBD) on changes renal transcriptome a mouse model. The hypothesis was systematic CBD would affect gene expression associated with those processes kidney. Methods: study conducted male C57BL/6J mice. Mice experimental groups received daily intraperitoneal injections at doses 10 mg/kg or 20 body weight (b.w.) 28 days. After experiment, tissues were collected, RNA isolated, RNA-Seq sequencing performed. Results: results show CBD’s expression, regulation genes related circadian rhythm (e.g., Ciart, Nr1d1, Nr1d2, Per2, Per3), glucocorticoid receptor function Cyp1b1, Ddit4, Foxo3, Gjb2, Pck1), lipid metabolism Cyp2d22, Cyp2d9, Decr2 Hacl1, Sphk1), inflammatory response Cxcr4 Ccl28). Conclusions: obtained suggest beneficial purposes treating disease, its should further analyzed clinical trials.

Language: Английский

Citations

0