Genome-Wide Analysis of the Serine Carboxypeptidase-like (SCPL) Protein Family of Bitter Gourd and Functional Validation of McSCPL22 in Fusarium oxysporum f. sp. Momordicae (FOM) Resistance DOI Open Access
Feng Guan, Xuetong Yang, Bo Shi

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(21), P. 11816 - 11816

Published: Nov. 3, 2024

Bitter gourd is increasingly being recognized for its value as a vegetable and medicinal use, but the molecular mechanisms of pathogen resistance remain relatively poorly understood. The serine carboxypeptidase-like (SCPL) protein family plays key role in plant growth, defense, so on. However, comprehensive identification functional characterization SCPL gene has yet to be conducted bitter melon. In this study, 32 genes were identified divided into three classes. number contained clusters was 7, 18, respectively. Most promoters contain cis-acting elements with light, hormone, stress responses. RNA sequencing data showed that expression several changed significantly after infection. particular, McSCPL4, 10, 17, 22, 25 increased substantially resistant varieties infection, their levels higher than those susceptible varieties. These results suggested such may play significant conferring fungal infections. Moreover, McSCPL10, 23, likewise induced by salicylic acid (SA) jasmonic (JA). situ hybridization McSCPL22 expressed vascular tissues infected plants, which largely overlapped location Fusarium oxysporum f. sp. Momordicae (FOM) infection site hydrogen peroxide production. Our involved regulation SA JA pathways enhance FOM plants. This first study perform analysis gourd. have potential gourd, further investigation function warranted. yield breeding

Language: Английский

Transcriptome analysis and non-target-site resistance mechanism of multi-resistant Lolium multiflorum under herbicide stress DOI Creative Commons

Hongle Xu,

Qiuli Leng,

Lanlan Sun

et al.

Current Plant Biology, Journal Year: 2025, Volume and Issue: unknown, P. 100441 - 100441

Published: Jan. 1, 2025

Language: Английский

Citations

0
Comparative Metabolome and Transcriptome Analysis Reveals the Possible Roles of Rice Phospholipase A Genes in the Accumulation of Oil in Grains DOI Open Access
Huasheng Cao,

Rong Gong,

Liang Xiong

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(21), P. 11498 - 11498

Published: Oct. 26, 2024

The phospholipase A (PLA) gene family plays a crucial role in the regulation of plant growth, development and stress response. Although PLA genes have been identified various species, their specific functions characteristics oil quality formation rice grains (Oryza sativa L.) not studied yet. Here, we characterized 35 genes, which were divided into three subgroups based on structures phylogenetic relationships. These are distributed unevenly across 11 chromosomes. promoter sequence PLAs contain multiple hormones stress-related elements. Gene expression analyses tissues under conditions indicated that may be involved In addition, metabolomics, transcriptomics qRT-PCR between two varieties Guang8B (G8B, high content) YueFengB (YFB, low revealed different expressional levels closely related to differences content ‘G8B’ ‘YFB’ grains. findings this study provide potential novel insights molecular information rice, underscore accumulation, providing valuable resources for future genetic improvement breeding strategies.

Language: Английский

Citations

0
Genome-Wide Analysis of the Serine Carboxypeptidase-like (SCPL) Protein Family of Bitter Gourd and Functional Validation of McSCPL22 in Fusarium oxysporum f. sp. Momordicae (FOM) Resistance DOI Open Access
Feng Guan, Xuetong Yang, Bo Shi

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(21), P. 11816 - 11816

Published: Nov. 3, 2024

Bitter gourd is increasingly being recognized for its value as a vegetable and medicinal use, but the molecular mechanisms of pathogen resistance remain relatively poorly understood. The serine carboxypeptidase-like (SCPL) protein family plays key role in plant growth, defense, so on. However, comprehensive identification functional characterization SCPL gene has yet to be conducted bitter melon. In this study, 32 genes were identified divided into three classes. number contained clusters was 7, 18, respectively. Most promoters contain cis-acting elements with light, hormone, stress responses. RNA sequencing data showed that expression several changed significantly after infection. particular, McSCPL4, 10, 17, 22, 25 increased substantially resistant varieties infection, their levels higher than those susceptible varieties. These results suggested such may play significant conferring fungal infections. Moreover, McSCPL10, 23, likewise induced by salicylic acid (SA) jasmonic (JA). situ hybridization McSCPL22 expressed vascular tissues infected plants, which largely overlapped location Fusarium oxysporum f. sp. Momordicae (FOM) infection site hydrogen peroxide production. Our involved regulation SA JA pathways enhance FOM plants. This first study perform analysis gourd. have potential gourd, further investigation function warranted. yield breeding

Language: Английский

Citations

0