Association of PD-1 + Treg/PD-1 + CD8 ratio and tertiary lymphoid structures with prognosis and response in advanced gastric cancer patients receiving preoperative treatment DOI Creative Commons
Xu Liu,

Danhua Xu,

Cheng‐Bei Zhou

et al.

Journal of Translational Medicine, Journal Year: 2024, Volume and Issue: 22(1)

Published: Dec. 27, 2024

Recent studies have highlighted the distinct ratio of PD-1 + Treg/PD-1 CD8 for prognosis prediction. However, it remains unclear about association this and tertiary lymphoid structures (TLS) with response to neoadjuvant or conversion therapy in advanced gastric cancer. Firstly, fresh postoperative samples from 68 cancer patients Renji Hospital were collected. Meanwhile, immune cell infiltration as well clinical analysis conducted. Subsequently, we further systematically evaluated flow cytometry tumor TLS expression 38 different situations after therapy. Also, a cohort including 10 complete matching before treatment was established receive RNA sequencing multiplex immunohistochemistry (mIHC) tests. The corresponding score compared based on therapeutic variations. In general, CD8>1 could be regarded an independent predictor patients. Moreover, < 1 high indicate better extended survival time advanved Besides, low &TLS group predict progression free (PFS) (CR) subgroup. therapy, number T cells significantly increased, mainly occurring outside TLSs. TLSs also considerably activated observed. This study underlined that combining associated preoperative Inspiringly, these indicators potential elucidate balance can accurately guide subsequent strategies.

Language: Английский

Human cytomegalovirus UL82 promotes cell cycle progression of colorectal cancer by upregulating AGR2 DOI Creative Commons
Haitao Ren, Bing Wang,

Lanni Wang

et al.

Communications Biology, Journal Year: 2025, Volume and Issue: 8(1)

Published: Feb. 17, 2025

The correlation between persistent human cytomegalovirus (HCMV) infection and poor prognosis in colorectal cancer (CRC) patients has garnered increasing attention. UL82 is a tegument protein of HCMV, our previous research indicated that the presence significantly associated with reduced overall survival CRC patients. However, mechanism by which affects remains unclear. In this study, we investigated role progression through both vitro vivo experiments, revealed its downstream regulatory pathways integrating transcriptomics, metabolomics, proteomics. Our findings first promoted cell proliferation proportion cells S phase cycle. Additionally, enhanced expression oncogene AGR2, while knockdown AGR2 abolished proliferative effect UL82. Interestingly, interacted transcription factor DDX5, transcriptionally inhibited expression. Furthermore, UL82-AGR2 axis nucleotide metabolism enhancing levels synthesis enzymes DTYMK, RRM2, TYMS. conclusion, study suggests UL82/DDX5 complex may promote cycle upregulating serve as potential prognostic biomarker for Human promotes AGR2.

Language: Английский

Citations

0
Purine Metabolism and Dystonia: Perspectives of a Long‐Promised Relationship DOI Creative Commons
Ugo Sorrentino, Audrey G. O’Neill, Justin M. Kollman

et al.

Annals of Neurology, Journal Year: 2025, Volume and Issue: unknown

Published: March 3, 2025

Dystonia research focuses on the identification of converging biological pathways, allowing to define molecular drivers that serve as treatment targets. We summarize evidence supporting concept aberrations in purine metabolism intersect with dystonia pathogenesis. The recent discovery IMPDH2 ‐related introduced a gain‐of‐function paradigm purinergic system defects, offering new perspectives understand purine‐pool imbalances brain diseases. discuss commonalities between known dystonia‐linked mechanisms and emerging from studies disorders including Lesch–Nyhan disease. Together, we hypothesize greater appreciation relevance perturbances can offer fresh avenues for therapeutic intervention. ANN NEUROL 2025

Language: Английский

Citations

0
Bioinformatics Analysis of Purine Metabolism Key Genes in Gastric Cancer Chemotherapy Resistance and Prognosis DOI

运 邵

Advances in Clinical Medicine, Journal Year: 2025, Volume and Issue: 15(03), P. 1023 - 1034

Published: Jan. 1, 2025

Language: Английский

Citations

0
The complex interplay between redox dysregulation and mTOR signaling pathway in cancer: A rationale for cancer treatment DOI
Christophe Glorieux, Cinthya Enríquez, Pedro Buc Calderón

et al.

Biochemical Pharmacology, Journal Year: 2024, Volume and Issue: 232, P. 116729 - 116729

Published: Dec. 19, 2024

Language: Английский

Citations

1
An Efficient One‐Pot Access of Pentoxifylline‐Thiazole Hybrids: Design, Synthesis, and Antioxidant Activity DOI

Jignesh Mehariya,

Umang Pithadiya,

Sabera Bijani

et al.

ChemistrySelect, Journal Year: 2024, Volume and Issue: 9(46)

Published: Dec. 1, 2024

Abstract An efficient, facile, and one‐pot route has been utilized for the synthesis of a new series 3,7‐dimethyl‐1‐(5‐(2‐(4‐substitutedphenylthiazol‐2‐yl)hydrazono)hexyl)‐1 H ‐purine‐2,6(3 ,7 )‐dione ( 4a – 4j ). The pentoxifylline 1 ) as novel precursor, thiosemicarbazide, variety phenacyl bromides have employed to generate newer pentoxifylline‐thiazole adduct using PPA green catalyst at reflux conditions afford high yield pure product. All synthesized compounds were purified chromatographic methods characterized by spectroscopic techniques 13 C NMR, MS, IR). Antioxidant activity was assessed DPPH free radical scavenging assay, it observed that with cyano functional groups electron‐donating showed notably higher potency, IC 50 values around 400 µM, while those halogens nitro displayed moderate efficacy ≈600 µM). These results compared standard ascorbic acid, which had an 382.54 ± 5.09 µM. Molecular docking performed on myeloperoxidase (MPO) enzyme explore mechanistic basis antioxidant activity, providing valuable insights into thermodynamic interactions contribute binding affinity.

Language: Английский

Citations

0
Association of PD-1 + Treg/PD-1 + CD8 ratio and tertiary lymphoid structures with prognosis and response in advanced gastric cancer patients receiving preoperative treatment DOI Creative Commons
Xu Liu,

Danhua Xu,

Cheng‐Bei Zhou

et al.

Journal of Translational Medicine, Journal Year: 2024, Volume and Issue: 22(1)

Published: Dec. 27, 2024

Recent studies have highlighted the distinct ratio of PD-1 + Treg/PD-1 CD8 for prognosis prediction. However, it remains unclear about association this and tertiary lymphoid structures (TLS) with response to neoadjuvant or conversion therapy in advanced gastric cancer. Firstly, fresh postoperative samples from 68 cancer patients Renji Hospital were collected. Meanwhile, immune cell infiltration as well clinical analysis conducted. Subsequently, we further systematically evaluated flow cytometry tumor TLS expression 38 different situations after therapy. Also, a cohort including 10 complete matching before treatment was established receive RNA sequencing multiplex immunohistochemistry (mIHC) tests. The corresponding score compared based on therapeutic variations. In general, CD8>1 could be regarded an independent predictor patients. Moreover, < 1 high indicate better extended survival time advanved Besides, low &TLS group predict progression free (PFS) (CR) subgroup. therapy, number T cells significantly increased, mainly occurring outside TLSs. TLSs also considerably activated observed. This study underlined that combining associated preoperative Inspiringly, these indicators potential elucidate balance can accurately guide subsequent strategies.

Language: Английский

Citations

0