Comparative Proteomics of Salinity Stress Responses in Fish and Aquatic Invertebrates

PROTEOMICS, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 9, 2025

Fluctuating salinity is symptomatic of climate change challenging aquatic species. The melting polar ice, rising sea levels, coastal surface and groundwater salinization, increased evaporation in arid habitats alter worldwide. Moreover, the frequency intensity extreme weather events such as rainstorms floods increase, causing rapid shifts brackish habitat salinity. Such alterations disrupt homeostasis ultimately diminish fitness, organisms by interfering with metabolism, reproduction, immunity, other critical aspects physiology. Proteins are central to these physiological mechanisms. They represent molecular building blocks phenotypes that govern organismal responses environmental challenges. Environmental cues regulate proteins a concerted fashion, necessitating holistic analyses proteomes for comprehending stress responses. Proteomics approaches reveal causes population declines enable bioindication geared toward timely interventions prevent local extinctions. effects on have been performed since mid-1990s, propelled invention two-dimensional protein gels, soft ionization techniques mass spectrometry (MS), nano-liquid chromatography 1970s 1980s. This review summarizes current knowledge regulation from organisms, including key methodological advances over past decades.

Language: Английский

The immunomodulation of outer membrane vesicles from Vibrio parahaemolyticus causing acute hepatopancreatic necrosis disease (VpAHPND) in Pacific white shrimp Litopenaeus vannamei

Fish & Shellfish Immunology, Journal Year: 2025, Volume and Issue: unknown, P. 110274 - 110274

Published: March 1, 2025

Language: Английский

Optimal Reference Genes for Gene Expression Analysis of Overmating Stress-Induced Aging and Natural Aging in Male Macrobrachium rosenbergii

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(8), P. 3465 - 3465

Published: April 8, 2025

Functional gene expression is closely linked to an organism's physiology and can be quantified using Real-Time Quantitative Reverse Transcription PCR (RT-qPCR). However, the stability of reference not absolute, which may impact accuracy RT-qPCR results. In this study, we evaluated suitability nine genes including receptor for activated protein kinase c1 (rack1), ribosomal L6 (rpl6), L9 (rpl9), S2 (rps2), S18 (rps18), lateral stalk subunit P0 (rplp0), eukaryotic translation elongation factor 1β (eef1b), initiation 4a (eif4a), 5a (eif5a) analyzed from RNA sequencing (RNA-Seq) data in addition three 1α (eef1a), β-actin (actb), glyceraldehyde 3-phosphate dehydrogenase (gapdh) selected literature obtain best internal controls analysis M. rosenbergii under overmating stress natural aging. RefFinder was used comprehensively evaluate candidate genes. The initial results showed that (eif5a, rps18, rplp0) RNA-Seq had relatively stable levels, were more than those commonly Eif5a rps18 combination Further indicated eif5a might study rosenbergii.

Language: Английский