Unraveling the nexus of oxidative stress, ocular diseases, and small extracellular vesicles to identify novel glaucoma biomarkers through in-depth proteomics DOI Creative Commons
Raquel Rejas‐González,

Ana Montero-Calle,

Natalia Pastora-Salvador

et al.

Redox Biology, Journal Year: 2024, Volume and Issue: 77, P. 103368 - 103368

Published: Sept. 21, 2024

Chronic ocular pathologies such as cataracts and glaucoma are emerging an important problem for public health due to the changes in lifestyle longevity. These age-related diseases largely mediated by oxidative stress. Small extracellular vesicles (sEVs) involved cell-to-cell communication transport. There is increasing interest about function of small eye. However, proteome content characterization sEVs released cells under pathological conditions not yet well known. Here, we aimed analyze protein profile intracellular from two cell lines (lens epithelial retinal ganglion cells) exposed stress identify altered proteins that could serve potential diagnostic biomarkers. The was analyzed quantitative mass spectrometry-based proteomics. Validation performed WB ELISA using extracts aqueous humor cataract patients. After data analysis, 176 7 dysregulated with expression ratio≥1.5 were identified lens cells' extract sEVs, respectively, upon induction. In cells, induction resulted dysregulation 1033 9 sEVs. addition, ELISA, mostly confirmed samples or patients comparison ICL individuals, RAD23B showing high ability. Importantly, this work expands knowledge provides new

Language: Английский

Extracellular Vesicles and Glaucoma: Opportunities and Challenges DOI
Mofazzal Hossain, Yutao Liu

Current Eye Research, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 10

Published: Feb. 3, 2025

Purpose Glaucoma is one of the leading causes irreversible blindness, characterized by progressive visual field loss. Several risk factors are associated with developing disease. However, exact mechanisms or pathological pathways involved still unknown. There an urgent need to find and biomarkers for early detection therapy halt progression cure Extracellular vesicles (EVs), specifically exosomes, have emerged as a crucial player in all aspects glaucoma, including pathogenesis therapeutic application their cell-cell communication properties.

Language: Английский

Citations

0
Identification of unique biomarkers for proliferative diabetic retinopathy with tractional retinal detachment by proteomics profiling of vitreous humor DOI Creative Commons
Yuyan Liu, Rongjie Wei, Yong Tang

et al.

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: April 18, 2025

Language: Английский

Citations

0
Unraveling the nexus of oxidative stress, ocular diseases, and small extracellular vesicles to identify novel glaucoma biomarkers through in-depth proteomics DOI Creative Commons
Raquel Rejas‐González,

Ana Montero-Calle,

Natalia Pastora-Salvador

et al.

Redox Biology, Journal Year: 2024, Volume and Issue: 77, P. 103368 - 103368

Published: Sept. 21, 2024

Chronic ocular pathologies such as cataracts and glaucoma are emerging an important problem for public health due to the changes in lifestyle longevity. These age-related diseases largely mediated by oxidative stress. Small extracellular vesicles (sEVs) involved cell-to-cell communication transport. There is increasing interest about function of small eye. However, proteome content characterization sEVs released cells under pathological conditions not yet well known. Here, we aimed analyze protein profile intracellular from two cell lines (lens epithelial retinal ganglion cells) exposed stress identify altered proteins that could serve potential diagnostic biomarkers. The was analyzed quantitative mass spectrometry-based proteomics. Validation performed WB ELISA using extracts aqueous humor cataract patients. After data analysis, 176 7 dysregulated with expression ratio≥1.5 were identified lens cells' extract sEVs, respectively, upon induction. In cells, induction resulted dysregulation 1033 9 sEVs. addition, ELISA, mostly confirmed samples or patients comparison ICL individuals, RAD23B showing high ability. Importantly, this work expands knowledge provides new

Language: Английский

Citations

2