Serum biomarker-based risk model construction for primary Sjögren’s syndrome with interstitial lung disease DOI Creative Commons
Xiaoli Liu, Xia Zhang, Juan Shi

et al.

Frontiers in Molecular Biosciences, Journal Year: 2024, Volume and Issue: 11

Published: Aug. 21, 2024

Background Cytokine network disturbances in primary Sjögren’s syndrome (pSS) have been reported many studies. However, their functions patients with and interstitial lung disease (pSS-ILD) is controversial. In this study, we aim to investigate the associations of immunological characteristics cytokine profiles pSS-ILD pathogenesis explore predictive values for pSS progression. Methods A total 256 initially diagnosed at Henan Provincial People’s Hospital were enrolled. After excluding previously various serious acute chronic respiratory system diseases cases other connective tissue or congenital heart diseases, 94 included further analysis, including 40 ILD 54 without (pSS-N-ILD). For comparison, 41 age- sex-matched healthy individuals as normal controls. Their clinical symptoms serological data cyclic citrullinated peptide (CCP) antibody (anti-CCP), antinuclear (ANA), anti-Ro52, anti-SSA, anti-SSB, C-reactive protein, IgG, IgM, IgA, C3, C4, 10 cytokines chemokines obtained. Wilcoxon test, chi-square Spearman correlation logistics regression analysis performed. Results Higher positive rates anti-SSB higher incidence dry cough, dyspnea, arthrosis shown than pSS-N-ILD cases. Anti-CCP antibodies (IL-1β, TNFα, IL-6, IL-5, IL-12p70, IL-17) higher, while C3 was lower Significant negative correlations IgG C4 IL-12p70 IL-17 IL-6 only patients. The anti-CCP positively correlated IL-5 patients, but not Multi-variable revealed combination anti-CCP, IL-17, effective predicting status Conclusion There significant differences serum marker levels between might be a potential risk predictor occurrence. involved development progression pSS-ILD. These results would provide new therapeutic targets treatment.

Language: Английский

Histological Indicators of Aging at the Cellular Level: Cellular Senescence, Fibrosis, and Regenerative Capacity DOI Creative Commons

Eman Ali Elkordy

Journal of Microscopy and Ultrastructure, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 9, 2025

Language: Английский

Citations

0
Integrating Metabolomics and Network Analyses to Explore Mechanisms of Geum japonicum var. chinense Against Pulmonary Fibrosis: Involvement of Arachidonic Acid Metabolic Pathway DOI Open Access

Junyan Ran,

Qian Wang, Tao Lu

et al.

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(4), P. 1462 - 1462

Published: Feb. 10, 2025

Pulmonary fibrosis (PF) emerges as a significant pulmonary sequelae in the convalescent phase of coronavirus disease 2019 (COVID-19), with current strategies neither specifically preventive nor therapeutic. Geum japonicum var. chinense (GJC) is used traditional Chinese medicine to effectively treat various respiratory conditions. However, protective effects GJC against PF remains unclear. In present study, anti-PF effect aqueous extract was studied using mouse model induced by bleomycin (BLM). To characterize metabolite changes related and reveal therapeutic targets for extract, we performed metabolomic network analysis on mice lungs. Finally, key were then validated Western blotting. alleviated onset progression lung inhibiting inflammatory responses regulating oxidative stress levels. Integrating serum metabolomics analyses showed arachidonic acid (AA) pathway be most important metabolic PF. Further validation AA protein levels rise ALOX5, PTGS2, CYP2C9, PLA2G2A treatment regulated above programming. conclusion, promising botanical drug delay mice. The primary mechanism action associated comprehensive regulation metabolites expression pathway.

Language: Английский

Citations

0
Green synthesized silver nanoparticles based on N-3-(dimethylamino)propyl methacrylamide/2-hydroxyethyl methacrylate hydrogels for antibacterial wound dressing material DOI
Elif Şeref, Özgür Özay

Journal of Macromolecular Science Part A, Journal Year: 2024, Volume and Issue: 61(9), P. 675 - 690

Published: Aug. 24, 2024

In this study, hydrogels were synthesized using Prunus persica ev. Bayramiç Beyazı extract as a crosslinker. N-3-(Dimethylamino)propyl methacrylamide (DMAPMA) and 2-Hydroxyethyl methacrylate (HEMA) monomers used in by redox polymerization. Hydrogel@Ag composites created synthesizing Ag0 nanoparticles within hydrogel network structures cross-linked with plant an in-situ green synthesis method. These characterized swelling, FTIR, TEM, XRD, TGA methods. The hydrogel@Ag loaded Naproxen Cefazolin drugs. in-vitro drug release profiles of the examined pH = 5.5 PBS environment, it was determined that approximately 75% drugs released 5 h. kinetics model for Higuchi model, followed Korsmeyer-Peppas model. nanoparticle encapsulation significantly affected degree swelling. MBA showed swelling capacity 370.16% mass, while fruit exhibited 1000.78% mass. silver-encapsulated demonstrated even higher 2313% Additionally, antibacterial tests against Staphylococcus aureus, Pseudomonas aeruginosa, Enterococcus faecalis microorganisms, Ag-free did not exhibit activity.

Language: Английский

Citations

1
Serum biomarker-based risk model construction for primary Sjögren’s syndrome with interstitial lung disease DOI Creative Commons
Xiaoli Liu, Xia Zhang, Juan Shi

et al.

Frontiers in Molecular Biosciences, Journal Year: 2024, Volume and Issue: 11

Published: Aug. 21, 2024

Background Cytokine network disturbances in primary Sjögren’s syndrome (pSS) have been reported many studies. However, their functions patients with and interstitial lung disease (pSS-ILD) is controversial. In this study, we aim to investigate the associations of immunological characteristics cytokine profiles pSS-ILD pathogenesis explore predictive values for pSS progression. Methods A total 256 initially diagnosed at Henan Provincial People’s Hospital were enrolled. After excluding previously various serious acute chronic respiratory system diseases cases other connective tissue or congenital heart diseases, 94 included further analysis, including 40 ILD 54 without (pSS-N-ILD). For comparison, 41 age- sex-matched healthy individuals as normal controls. Their clinical symptoms serological data cyclic citrullinated peptide (CCP) antibody (anti-CCP), antinuclear (ANA), anti-Ro52, anti-SSA, anti-SSB, C-reactive protein, IgG, IgM, IgA, C3, C4, 10 cytokines chemokines obtained. Wilcoxon test, chi-square Spearman correlation logistics regression analysis performed. Results Higher positive rates anti-SSB higher incidence dry cough, dyspnea, arthrosis shown than pSS-N-ILD cases. Anti-CCP antibodies (IL-1β, TNFα, IL-6, IL-5, IL-12p70, IL-17) higher, while C3 was lower Significant negative correlations IgG C4 IL-12p70 IL-17 IL-6 only patients. The anti-CCP positively correlated IL-5 patients, but not Multi-variable revealed combination anti-CCP, IL-17, effective predicting status Conclusion There significant differences serum marker levels between might be a potential risk predictor occurrence. involved development progression pSS-ILD. These results would provide new therapeutic targets treatment.

Language: Английский

Citations

0