Exploring Morphology of Thermoplasmonic Nanoparticles to Synergize Immunotherapeutic Fibroblast Activation Protein‐Positive Cell Sensitization and Photothermal Therapy DOI Creative Commons
Ahmed Alsadig, Xuan Peng, Hugo Boutier

et al.

Small Science, Journal Year: 2025, Volume and Issue: unknown

Published: May 26, 2025

The precision of photothermal therapy (PTT) is often hindered by the challenge achieving selective delivery thermoplasmonic nanostructures to tumors. Active targeting, which leverages synthetic molecular complexes address receptors overexpressed malignant cells, enables such specificity and facilitates combination PTT with other anticancer therapies. In this study, we developed nanoconjugates consisting (i) 20 nm spherical gold nanoparticles (AuNPs) or nanostars (AuNSs) as nanocarriers, (ii) surface‐passivated antibody‐based fibroblast activation protein (FAP)‐targeting modules, used in adaptive chimeric antigen receptor T‐cells immunotherapy. demonstrated excellent stability specific binding FAP‐expressing fibrosarcoma HT1080 genetically modified express human FAP, confirmed fluorescence activated cell sorting, immunofluorescence, surface plasmon resonance scattering imaging. Moreover, nanocarriers showed significant conversion after visible near‐infrared irradiation. Quantitative thermal lens spectroscopy superior capability AuNSs, up 1.5‐fold greater enhancement than AuNPs under identical conditions. This synergistic approach, combining targeted immunotherapy not only streamlines nanoparticle delivery, increasing yield therapeutic efficacy but also offers a comprehensive potent strategy for cancer treatment potential outcomes across multiple modalities.

Language: Английский

CAR T cells re-directed by a rationally designed human peptide tag demonstrate efficacy in pre-clinical models DOI

Nele Knelangen,

Ulrika Bader,

Evangelia Maniaki

et al.

Cytotherapy, Journal Year: 2025, Volume and Issue: unknown

Published: March 1, 2025

Language: Английский

Citations

0
Role of CAR-T cell K+ channels in tumor infiltration and elimination DOI Creative Commons
Vivien Jusztus, Árpád Szöőr, Péter Hajdú

et al.

The Journal of Immunology, Journal Year: 2025, Volume and Issue: unknown

Published: May 15, 2025

Abstract Genetic modification of T cells to express chimeric antigen receptors (CAR, CAR-T cells) enable them recognize the specific on tumor surface and then eliminate tumor. lymphocyte ion channels such as Kv1.3, KCa3.1 CRAC influence cell activation proliferation by regulating Ca2+ signaling, well other effector functions cytokine release, migration even target killing. Here we established two CAR lines (using CEM line primary recognizing CD19 Raji B human breast cancer MCF-7 expressing lines. First, exposed that Kv1.3 functional expressions were comparable those in cells, which demonstrated their suitability for mimics. Next, studied killing efficiency CEM-CAR monolayer 3D spheroid models. We could show specifically regardless Furthermore, application (Vm24) (TRAM34) inhibitors significantly improved eradication both spheroids, however, infiltration rate was not influenced upon addition antagonists. conclude contribute a more effective immunotherapy solid

Language: Английский

Citations

0
Exploring Morphology of Thermoplasmonic Nanoparticles to Synergize Immunotherapeutic Fibroblast Activation Protein‐Positive Cell Sensitization and Photothermal Therapy DOI Creative Commons
Ahmed Alsadig, Xuan Peng, Hugo Boutier

et al.

Small Science, Journal Year: 2025, Volume and Issue: unknown

Published: May 26, 2025

The precision of photothermal therapy (PTT) is often hindered by the challenge achieving selective delivery thermoplasmonic nanostructures to tumors. Active targeting, which leverages synthetic molecular complexes address receptors overexpressed malignant cells, enables such specificity and facilitates combination PTT with other anticancer therapies. In this study, we developed nanoconjugates consisting (i) 20 nm spherical gold nanoparticles (AuNPs) or nanostars (AuNSs) as nanocarriers, (ii) surface‐passivated antibody‐based fibroblast activation protein (FAP)‐targeting modules, used in adaptive chimeric antigen receptor T‐cells immunotherapy. demonstrated excellent stability specific binding FAP‐expressing fibrosarcoma HT1080 genetically modified express human FAP, confirmed fluorescence activated cell sorting, immunofluorescence, surface plasmon resonance scattering imaging. Moreover, nanocarriers showed significant conversion after visible near‐infrared irradiation. Quantitative thermal lens spectroscopy superior capability AuNSs, up 1.5‐fold greater enhancement than AuNPs under identical conditions. This synergistic approach, combining targeted immunotherapy not only streamlines nanoparticle delivery, increasing yield therapeutic efficacy but also offers a comprehensive potent strategy for cancer treatment potential outcomes across multiple modalities.

Language: Английский

Citations

0