Root-associated microbial diversity and metabolomics in maize resistance to stalk rot DOI Creative Commons
Liming Wang, Jiao Jia,

Qianfu Su

et al.

Frontiers in Microbiology, Journal Year: 2024, Volume and Issue: 15

Published: Dec. 12, 2024

As one of the three major food crops in world, maize plays a significant role alleviating crisis. Maize stalk rot can reduce yield and mechanical harvesting efficiency. In addition, mycotoxins such as Deoxynivalenol (DON) Zearalenone (ZEN) produced by pathogens also harm livestock human health. is an infection whole growth period, there are no effective control measures at present. Therefore, it great to study pathogenesis mechanism from multiple perspectives. present study, root rhizosphere soil disease-resistant inbred line Y853 disease-susceptible Q478 were collected dough stage (R4) maturity (R6) maize, respectively. The effects resistant/susceptible on microorganisms analyzed amplicon sequences metabolomics. results showed that was different microbial community composition lines stages. Specifically, abundance Arthrobacter , Streptomyces Bacillus R4 higher than R6, while fungal LR853 significantly other compartments. Co-occurrence network analysis pathogen Fusarium had highest degree centrality closeness DR478. Moreover, metabolomics four main metabolic pathways enriched, 15 metabolites upgrade resistant line. Furthermore, microbes, especially fungi, related these metabolites. Our revealed resistance closely root-associated microbiota rhizospheric metabolites, which would be new perspective phytopathogenic biocontrol.

Language: Английский

The family of glutathione peroxidase proteins and their role against biotic stress in plants: a systematic review DOI Creative Commons
Maria Luíza do Carmo Santos, Ariana Silva Santos, Diogo Pereira Silva de Novais

et al.

Frontiers in Plant Science, Journal Year: 2025, Volume and Issue: 16

Published: Feb. 20, 2025

Glutathione peroxidases (GPXs) are extensively studied for their indispensable roles in eliminating reactive oxygen species by catalyzing the reduction of hydrogen peroxide or lipid peroxides to prevent cell damage. However, knowledge GPXs plants still has many gaps be filled. Thus, we present first systematic review (SR) aimed at examining function and protective role against death subjected biotic stress. To guide SR avoid bias, a protocol was developed that contained inclusion exclusion criteria based on PRISMA guidelines. Three databases (PubMed, Science Direct, Springer) were used identify relevant studies this research selected. A total 28 articles related proposed objective. The results highlight importance plant defense stress, including protecting death, similar anti-apoptotic animals. Data from gene expression protein accumulation under various stresses reveal can both increase resistance susceptibility pathogens. In addition antioxidant functions, act as sensors transmitters H2O2 signals, integrating with ABA signaling pathway during These findings show delay senescence reinforce physical barriers, thereby modulating Additionally, functions linked cellular localization, which demonstrates an evolutionary relationship between isoforms defense. This information broadens understanding molecular strategies involving GPX provides foundation discussions actions controlling necrotrophic and/or hemibiotrophic

Language: Английский

Citations

1
Combining Linkage and Association Mapping Approaches to Study the Genetic Architecture of Verticillium Wilt Resistance in Sunflower DOI Creative Commons
Juan F. Montecchia,

Mónica Fass,

Matías Dominguez

et al.

Plants, Journal Year: 2025, Volume and Issue: 14(8), P. 1187 - 1187

Published: April 11, 2025

Sunflower Verticillium Wilt and Leaf Mottle (SVW), caused by dahliae Kleb., is a globally prevalent disease affecting sunflower production. In this study, we identified major quantitative trait locus (QTL) on chromosome 10 other genomic regions associated with SVW resistance integrating biparental association mapping in populations from the National Institute of Agricultural Technology. Nine replicated field trials were conducted highly infested V. reservoirs to assess incidence severity. Both genotyped using double-digest restriction-site-associated DNA sequencing (ddRADseq). Association 18,161 SNPs QTL 1769 explaining up 30% phenotypic variation for at flowering area under progress curve incidence, which contributes lesser extent severity reduction. Additional QTLs chromosomes 17, 8, 9, 14, 13, 11 reduced severity, or both. Candidate genes within these regions, 39 are Chromosome 10. These findings demonstrate value complementary strategies validating loci advancing breeding resistance.

Language: Английский

Citations

0
Fatty Acid ABCG Transporter GhSTR1 Mediates Resistance to Verticillium dahliae and Fusarium oxysporum in Cotton DOI Creative Commons
George Z. Cheng,

Xiuqing Li,

W. G. Dilantha Fernando

et al.

Plants, Journal Year: 2025, Volume and Issue: 14(3), P. 465 - 465

Published: Feb. 5, 2025

Verticillium wilt and Fusarium cause significant losses in cotton (Gossypium hirsutum) production have a economic impact. This study determined the functional role of GhSTR1, member ABCG subfamily ATP-binding cassette (ABC) transporters, that mediates defense responses against various plant pathogens. We identified GhSTR1 as homolog STR1 from Medicago truncatula highlighted its evolutionary conservation potential mechanisms. Expression profiling revealed displays tissue-specific spatiotemporal dynamics under stress conditions caused by dahliae oxysporum. Functional validation using virus-induced gene silencing (VIGS) showed improved disease resistance, resulting milder symptoms, less vascular browning, reduced fungal growth. Furthermore, AtSTR1 loss-of-function mutant Arabidopsis thaliana exhibited similar resistance phenotypes, highlighting conserved regulatory pathogen defense. In addition to mutation also enhanced vegetative reproductive growth plant, including increased root length, rosette leaf number, height without compromising drought tolerance. These findings suggest trade-off between growth, offering target for optimizing both traits crop improvement. identifies key regulator plant–pathogen interactions dynamics, providing foundation developing durable strategies enhance cotton’s yield biotic abiotic conditions.

Language: Английский

Citations

0
What Happens Inside the Germinating Grain After Microbial Decontamination by Pulsed Electric Field? Data-Driven Multi-Omics Helps Find the Answer DOI Creative Commons
Milena Stránská, Adam Behner, Jaroslava Ovesná

et al.

Molecules, Journal Year: 2025, Volume and Issue: 30(4), P. 924 - 924

Published: Feb. 17, 2025

Pulsed electric field (PEF) has previously been recognized as a method of gentle food processing, and its use shown to be helpful in reducing the levels toxigenic Fusarium micromycetes developed during malting. The aim this study was describe effects PEF on gene expression metabolite production at pre-finishing stage barley malting by using novel multi-omics data-driven approach. helps uncover processes occurring germinated grain discusses up-/downregulation genes metabolites relation fungal infection and/or PEF-induced abiotic stress. Among factors upregulated described supportive against diseases, we identified increased encoding vegetative gp1-like protein, which positively correlated with flavonoids, (methylsulfanyl)prop-2-enoates, triterpenoid glycosides, indole alkaloids. On other hand, some associated resistance were also overexpressed untreated control (in particular, ethylene response factor 3-like, putrescine hydroxycinnamoyltransferase dirigent protein 21-like). This provides first ‘data-driven’ basic research results that contribute understanding role an effective decontamination strategy allows formulation new hypotheses related pathogen crosstalk.

Language: Английский

Citations

0
OsMbl1 Counteracts OsGdsl1‐Mediated Rice Blast Susceptibility by Inhibiting Its Lipase Activity DOI
Linlin Song, Tao Yang, Yakubu Saddeeq Abubakar

et al.

Plant Cell & Environment, Journal Year: 2025, Volume and Issue: unknown

Published: April 15, 2025

ABSTRACT Plant lectins have a significant impact on the defense against pathogens and insect attacks. The jacalin‐related lectin OsMbl1 from rice ( Oryza sativa L.) has been reported to play crucial role in pattern‐triggered immunity (PTI). However, underlying mechanism remains unclear. In this study, we identified GDSL‐like lipase, OsGdsl1, that interacts with both vitro vivo. OsGdsl1 protein, which lipase activity, is localized lipid bodies apoplast. expression of OsGDSL1 modulated upon exposure Magnaporthe oryzae M. ) or plant hormones. Deletion gene not only improved resistance , but also led an increased ROS burst after chitin treatments. some pathogenesis‐related (PR) genes was upregulated mutants. We found inhibited activity during infection . Overall, our results suggest negatively regulates by downregulating bursts PR expressions, while its OsMbl1, contributes enhancement innate infection.

Language: Английский

Citations

0
Root-associated microbial diversity and metabolomics in maize resistance to stalk rot DOI Creative Commons
Liming Wang, Jiao Jia,

Qianfu Su

et al.

Frontiers in Microbiology, Journal Year: 2024, Volume and Issue: 15

Published: Dec. 12, 2024

As one of the three major food crops in world, maize plays a significant role alleviating crisis. Maize stalk rot can reduce yield and mechanical harvesting efficiency. In addition, mycotoxins such as Deoxynivalenol (DON) Zearalenone (ZEN) produced by pathogens also harm livestock human health. is an infection whole growth period, there are no effective control measures at present. Therefore, it great to study pathogenesis mechanism from multiple perspectives. present study, root rhizosphere soil disease-resistant inbred line Y853 disease-susceptible Q478 were collected dough stage (R4) maturity (R6) maize, respectively. The effects resistant/susceptible on microorganisms analyzed amplicon sequences metabolomics. results showed that was different microbial community composition lines stages. Specifically, abundance Arthrobacter , Streptomyces Bacillus R4 higher than R6, while fungal LR853 significantly other compartments. Co-occurrence network analysis pathogen Fusarium had highest degree centrality closeness DR478. Moreover, metabolomics four main metabolic pathways enriched, 15 metabolites upgrade resistant line. Furthermore, microbes, especially fungi, related these metabolites. Our revealed resistance closely root-associated microbiota rhizospheric metabolites, which would be new perspective phytopathogenic biocontrol.

Language: Английский

Citations

1