Optimization of the Preparation Process and Ameliorative Efficacy in Osteoporotic Rats of Peptide–Calcium Chelates from Skipjack Tuna (Katsuwonus pelamis) Meat

Foods, Journal Year: 2024, Volume and Issue: 13(17), P. 2778 - 2778

Published: Aug. 30, 2024

This study aimed to establish the preparation process of peptide–calcium chelates (TMP-Ca) using skipjack tuna meat and investigate function mechanism TMP-Ca in an osteoporosis model rats. The results indicated that trypsin is more suitable for preparing Ca-chelating hydrolysates meat, optimal hydrolysis conditions were derived as follows: digestion time 4 h, material–liquid ratio 1:10, enzyme dose 3%. chelating Ca with hydrolysate optimized be chelation 50 min, temperature °C, pH 8.0, a peptide–Ca 1:10. prepared was subjected ultrafiltration, fraction (TMP) (MW <1 kDa) showed highest rate (51.27 ± 1.42%) made into (TMP-Ca). In osteoporotic rats, significantly improved decrease ovarian indexes caused by retinoic acid. It also elevated serum Ca, phosphorus, bone turnover indexes, increased number trabeculae, microstructure. addition, we confirmed could regulate OPG/TRAF6 pathway reduce osteoclast differentiation, inhibit resorption, promote formation. Therefore, ameliorate osteoporosis, this provides functional component healthcare products treat osteoporosis.

Language: Английский

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Peptide Fractions Extracted from the Hemolymph of Hermetia illucens Inhibit Growth and Motility and Enhance the Effects of Traditional Chemotherapeutics in Human Colorectal Cancer Cells

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(5), P. 1891 - 1891

Published: Feb. 22, 2025

Cancer is a leading cause of death worldwide, characterized by uncontrolled cell growth and multiple mutations. Chemotherapy often associated with harmful side effects, cancer cells may become resistant through various mechanisms. New approaches, which are able to address both the toxicity resistance issues chemotherapy, primary importance in research. Antimicrobial peptides (AMPs), naturally occurring molecules innate immune system all living organisms, have wide spectrum cytotoxic activities against could be promising alternative actual chemotherapeutics. Here, we tested peptide fractions, rich AMPs, extracted from hemolymph larvae insect Hermetia illucens on HT29 HCT116 human colorectal cells, observing inhibition accumulation G2/M phase increased apoptosis. Furthermore, extract induced significant cytoskeleton reorganization, resulting reduced motility. These effects were more evident fractions obtained Escherichia coli-infected larvae. The also enhanced traditional Overall, results suggest presence biologically active H. larvae, confirming that insect-derived research area oncology.

Language: Английский

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Peptides as Versatile Regulators in Cancer Immunotherapy: Recent Advances, Challenges, and Future Prospects

Pharmaceutics, Journal Year: 2025, Volume and Issue: 17(1), P. 46 - 46

Published: Jan. 1, 2025

The emergence of effective immunotherapies has revolutionized therapies for many types cancer. However, current immunotherapy limited efficacy in certain patient populations and displays therapeutic resistance after a period treatment. To address these challenges, growing number drugs have been investigated clinical preclinical applications. diverse functionality peptides made them attractive as modality, the global market peptide-based therapeutics is witnessing significant growth. Peptides can act immunotherapeutic agents treatment malignant cancers. systematic understanding interactions between different host’s immune system remains unclear. This review describes detail roles regulating function cancer immunotherapy. Initially, we systematically elaborate on relevant mechanisms Subsequently, categorize nanomaterials into following three categories: vaccines, anti-cancer peptides, delivery systems. We carefully analyzed overcoming barriers immunotherapy, including multiple strategies to enhance immunogenicity peptide synergistic effect combination with other agents, assemblies functioning stimulators or vehicles deliver agents. Furthermore, introduce status applications discuss weaknesses future prospects materials Overall, this aims comprehension potential lay groundwork research

Language: Английский

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Efficient Synthesis, Stability-Guided Optimization and Anticancer Evaluation of Bee Venom Peptide Melittin

Published: Jan. 1, 2025

Natural cytotoxic peptides (NCPs) are emerging sources of novel anticancer chemotherapeutics. Especially, Melittin, which is the major component bee venom and first-in-class NCP, has been considered as a promising scaffold. Nevertheless, classical linear, cationic, amphipathic, membrane-lytic peptide, Melittin may be easily degraded by proteases, suffering from poor stability, moderate durability, severe hemolysis. In this study, applying terminal modification hybridization strategies, ten Melittin-based derivatives were designed, synthesized, investigated for their potential. The robust economic synthetic method, in vitro efficiency, time-kill kinetics, serum anti-migration activity, hemolysis effects, mechanism explored. As expected, exhibited highly potent cytotoxicity against all six tested cancer cell lines. particular, compared with natural derived LJ-5 containing both N-terminal acetylation C-terminal hydrazidation, LJ-6, methotrexate MTX-GFLG-Melittin conjugate significantly improved proteolytic more durable higher well reduced effects. Besides, it was further verified that LJ-6 could efficiently disrupt integrity membrane, localize to mitochondria rapidly reduce mitochondrial membrane potential cells. Collectively, method stability-guided optimization conducted on affording hydrolysis-resistant serve candidates useful references optimizations peptides.

Language: Английский

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Efficient synthesis, stability-guided optimization and anticancer evaluation of bee venom peptide Melittin

Bioorganic Chemistry, Journal Year: 2025, Volume and Issue: 159, P. 108344 - 108344

Published: March 4, 2025

Language: Английский

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Identification, characterization, and molecular docking of immunomodulatory peptides in Astragalus (Astragalus membranaceus (Fisch.)Bge) seed protein hydrolysates

Food Chemistry, Journal Year: 2025, Volume and Issue: 480, P. 143631 - 143631

Published: March 8, 2025

Language: Английский

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RAS70 peptide targets multiforme glioblastoma by binding to the plasma membrane heat shock protein HSP70

Frontiers in Oncology, Journal Year: 2025, Volume and Issue: 15

Published: March 24, 2025

Multiforme glioblastoma-homing peptides, particularly targeting plasma membrane-bound heat shock protein mHsp70, demonstrate great application potential for tumor theranostics. In the current study, to further increase bioavailability as well penetration capacity through blood-brain barrier (BBB) of mHsp70-targeted peptide TKDNNLLGRFELSG, which is known bind oligomerization sequence mHsp70 chaperone, latter was conjugated with tripeptide RGD (forming chimeric termed RAS70). model BBB system RAS70 efficiently crossed accumulating in glioblastoma cells. Subsequently, orthotopic glioma models, intravenous administration fluorescently labeled agent (RAS70-sCy7.5) resulted retention (further confirmed by histological studies). Thus, shown biodistribution studies employing epifluorescence imaging, accumulation RAS70-sCy7.5 C6 significantly enhanced compared scramble peptide. Local that sprayed over dissected brain tissues helped delineate tumors glioma-bearing animals an intraoperative fluorescent imaging system. Tumor-specific internalization on ex vivo primary GBM samples obtained from adult neurooncological patients. conclusion, demonstrated high glioma-homing properties could be employed visualization developing a carrier drug delivery.

Language: Английский

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A new short pH-responsive anticancer peptide derived by intramolecular charge shielding strategy

European Journal of Medicinal Chemistry, Journal Year: 2025, Volume and Issue: 291, P. 117662 - 117662

Published: April 19, 2025

Language: Английский

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Antitumoral and Antiproliferative Potential of Synthetic Derivatives of Scorpion Peptide IsCT1 in an Oral Cavity Squamous Carcinoma Model

Molecules, Journal Year: 2024, Volume and Issue: 29(19), P. 4533 - 4533

Published: Sept. 24, 2024

The oral cavity is a frequent site for head and neck cancers, which rank as the sixth most common cancer globally, with 5-year survival rate slightly over 50%. Current treatments are limited, resistance to therapy remains significant clinical obstacle. IsCT1, membrane-active peptide derived from venom of scorpion Opisthacanthus madagascariensis, has shown antitumor effects in various cell lines, including breast chronic myeloid leukemia. However, its hemolytic action limits potential therapeutic use. This study aims assess antiproliferative activities synthetic peptides IsCT1 (IsCT-P, AC-AFPK-IsCT1, AFPK-IsCT1, AC-KKK-IsCT1, KKK-IsCT1) context squamous carcinoma. We evaluated cytotoxic these on tongue carcinoma cells normal cells, well their impact cycle phases, expression proliferation markers, modulators death pathways, mitochondrial potential. Our results indicate that derivatives IsCT-P AC-AFPK-IsCT1 possess properties towards reducing membrane proliferative index. treatment led positive modulation pro-apoptotic markers p53 caspases 3 8, decrease PCNA Cyclin D1 expression, arrest S phase. Notably, contrary parental peptide, did not exhibit activity or cytotoxicity cells. Therefore, might be viable option treatment.

Language: Английский

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Anticancer Ribosomally Synthesized and Post-Translationally Modified Peptides from Plants: Structures, Therapeutic Potential, and Future Directions

Current Issues in Molecular Biology, Journal Year: 2024, Volume and Issue: 47(1), P. 6 - 6

Published: Dec. 26, 2024

Cancer remains a significant medical challenge, necessitating the discovery of novel therapeutic agents. Ribosomally synthesized and post-translationally modified peptides (RiPPs) from plants have emerged as promising source anticancer compounds, offering unique structural diversity potent biological activity. This review identifies discusses cytotoxic RiPPs across various plant families, focusing on their absolute chemical structures reported activities against cancer cell lines. Notably, plant-derived such rubipodanin A mallotumides A-C demonstrated low nanomolar IC50 values multiple types, highlighting potential. By integrating traditional ethnobotanical knowledge with modern genomic bioinformatic approaches, this study underscores importance resource for developing innovative treatments. These findings pave way further exploration RiPPs, emphasizing role in addressing ongoing challenges oncology enhancing repertoire effective therapies.

Language: Английский

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