Brown adipose tissue transplantation ameliorates hindlimb ischemic damage in diabetic mice

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: March 14, 2025

Peripheral arterial disease (PAD) is a common complication associated with diabetes, which can lead to foot ischemia. The condition often accompanied by infection and necrosis, ultimately leading diabetic ulcers the risk of amputation. Brown adipose tissue (BAT) its secreted cytokines play an essential role in regulation glucose homeostasis, modulation inflammatory responses, vascular endothelial cell proliferation. transplantation BAT into ischemic regions may offer therapeutic benefits alleviating symptoms PAD. A mouse model was established via intraperitoneal administration streptozocin. Subsequently, lower limb ulcer constructed transection femoral artery ligation vein. harvested from subscapular region employed as graft. research utilized Laser Doppler monitoring, Western blot analysis, hematoxylin-eosin (HE) staining, immunofluorescence enzyme-linked immunosorbent assay (ELISA) evaluate blood flow recovery regions, histopathological changes, angiogenesis remodeling, M1/M2 macrophage polarization. significantly enhanced mice while concurrently reducing necrotic tissue. Pathological analyses demonstrate that mitigates damage, stimulates angiogenesis, supports remodeling. Furthermore, blotting, immunofluorescence, ELISA results revealed reduces levels tissues, increases expression angiogenic factors, promotes polarization macrophages M1 M2 phenotype. has demonstrated mitigate injury mice, attenuate facilitate restoration flow. These effects be linked alterations

Language: Английский

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Mapping growth differentiation factor-15 (GDF15)-mediated signaling pathways in cancer: insights into its role across different cancer types

Discover Oncology, Journal Year: 2025, Volume and Issue: 16(1)

Published: March 25, 2025

Growth differentiation factor-15 (GDF15) is a cytokine/growth factor that belongs to the Transforming growth factor-ß (TGF-ß) protein family. The expression of GDF15 low in most human organs under normal conditions. stress-responsive cytokine primarily produced by macrophages response inflammatory stimuli. altered associated with many cancers due inflammation caused disease. triggers activity through its receptor Glial-derived neurotrophic factor-family α-like (GFRAL) and mediates multiple downstream signaling cascades, which are involved progression cancers. Considering biological importance different cancers, we applied data mining techniques systematically compile analyze events using NetPath criteria. This resulted constructing detailed GDF15-mediated pathway map, enhancing our understanding molecular mechanisms cancer. Furthermore, proteins linked colorectal breast cancer identified map were cross-referenced established databases identify unannotated proteins, highlighting gaps current annotations. To investigate potential therapeutic strategies, performed docking simulations Vitisifuran B as novel inhibitor could block GDF15-GFRAL interaction. These findings suggest effectively modulate signaling, offering promising avenue for therapeutics. study underscores power computational approaches, such docking, identifying inhibitors development.

Language: Английский

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Revisiting the role of GDF15 in atherosclerosis in mouse and human

Acta Pharmacologica Sinica, Journal Year: 2025, Volume and Issue: unknown

Published: April 30, 2025

Language: Английский

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Growth differentiation factor 15 as a potential diagnostic biomarker for rheumatoid arthritis

Bone and Joint Research, Journal Year: 2025, Volume and Issue: 14(5), P. 389 - 397

Published: May 6, 2025

Aims This systematic review aimed to investigate the association between growth differentiation factor 15 (GDF-15) and rheumatoid arthritis (RA) disease activity, explore differences at genetic level, evaluate value of GDF-15 in diagnosing RA. Methods A comprehensive literature search was conducted using PubMed, Web Science, Cochrane Library, Embase on 23 August 2023. Methodological quality independently assessed by Agency for Healthcare Research Quality scale. The primary parameters analyzed were serum concentration, diagnostic sensitivity specificity. Results total 469 documents retrieved, five clinical studies ultimately included. In included studies, levels found be notably greater RA patients than healthy individuals, these exhibited a positive correlation with severity. Furthermore, increased associated specific gene variations patients, but varied according ethnicity. two showed high specificity highly active RA, demonstrating its utility as biomarker Conclusion expression is activity; thus, potentially an effective However, additional high-quality especially randomized controlled trials cohort follow-up data, are needed assess role Cite this article: Bone Joint Res 2025;14(5):389–397.

Language: Английский

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Serum growth differentiation factor-15, glial fibrillary acidic protein, and neurofilament light chain: Their link and role in Creutzfeldt-Jakob disease

Journal of the Neurological Sciences, Journal Year: 2024, Volume and Issue: 467, P. 123305 - 123305

Published: Nov. 13, 2024

Creutzfeldt-Jakob disease (CJD) is a rapidly progressive neurodegenerative disorder characterized by neuronal damage. Emerging biomarkers, such as serum neurofilament light chain (sNfL), glial fibrillary acidic protein (sGFAP), and growth differentiation factor-15 (sGDF-15), are currently being studied for their potential use in this disease.

Language: Английский

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