Exploring Bone Morphogenetic Protein-2 and -4 mRNA Expression and Their Receptor Assessment in a Dynamic In Vitro Model of Vascular Calcification DOI Creative Commons
Manuela Cabiati, Federico Vozzi, Elisa Ceccherini

et al.

Cells, Journal Year: 2024, Volume and Issue: 13(24), P. 2091 - 2091

Published: Dec. 18, 2024

Background: Vascular calcification (VC) is a dynamic, tightly regulated process driven by cellular activity and resembling the mechanisms of bone formation, with specific molecules playing pivotal roles in its progression. We aimed to investigate involvement morphogenic proteins (BMP-2, BMP-4, BMPR-1a/1b, BMPR-2) system this process. Our study used an advanced vitro model that simulates biological environment vascular wall, assessing ability phosphate mixture induce osteoblastic switch human coronary artery smooth muscle cells (HCASMCs). Methods: HCASMCs were grown mono- co-culture endothelial (HCAECs) double-flow bioreactor (LiveBox2 IVTech), allowing static dynamic conditions through peristaltic pump. The VC was stimulated incubation calcifying medium for 7 days. A BMP Real-Time PCR performed at end each experiment. Results: In monocultures, BMP-2 expression increased calcified (p = 0.01) conditions. BMP-4 receptors expressed all experimental settings, increasing mainly flow co-cultures, we observed marked increase BMPR-1a 0.04 p 0.01, respectively), BMPR-2 0.001) setting mostly Conclusions: BMP-2/4 suggests these genes might promote towards osteogenic-like phenotype, data also supported rise both BMPR-2. Thus, our findings provide insights into which modulates activation mimicking vivo VC’s mechanical characteristics.

Language: Английский

In Vitro Models of Cardiovascular Calcification DOI Creative Commons
Andrea Tóth, Enikő Balogh, Viktória Jeney

et al.

Biomedicines, Journal Year: 2024, Volume and Issue: 12(9), P. 2155 - 2155

Published: Sept. 23, 2024

Cardiovascular calcification, characterized by hydroxyapatite deposition in the arterial wall and heart valves, is associated with high cardiovascular morbidity mortality. calcification a hallmark of aging but frequently seen association chronic diseases, such as kidney disease (CKD), diabetes, dyslipidemia, hypertension younger population well. Currently, there no therapeutic approach to prevent or cure calcification. The pathophysiology highly complex involves osteogenic differentiation various cell types system, vascular smooth muscle cells valve interstitial cells. In vitro cellular ex vivo tissue culture models are simple useful tools research. These contributed largely discoveries numerous inducers, inhibitors, molecular mechanisms. this review, we provide an overview applied research

Language: Английский

Citations

2
Exploring Bone Morphogenetic Protein-2 and -4 mRNA Expression and Their Receptor Assessment in a Dynamic In Vitro Model of Vascular Calcification DOI Creative Commons
Manuela Cabiati, Federico Vozzi, Elisa Ceccherini

et al.

Cells, Journal Year: 2024, Volume and Issue: 13(24), P. 2091 - 2091

Published: Dec. 18, 2024

Background: Vascular calcification (VC) is a dynamic, tightly regulated process driven by cellular activity and resembling the mechanisms of bone formation, with specific molecules playing pivotal roles in its progression. We aimed to investigate involvement morphogenic proteins (BMP-2, BMP-4, BMPR-1a/1b, BMPR-2) system this process. Our study used an advanced vitro model that simulates biological environment vascular wall, assessing ability phosphate mixture induce osteoblastic switch human coronary artery smooth muscle cells (HCASMCs). Methods: HCASMCs were grown mono- co-culture endothelial (HCAECs) double-flow bioreactor (LiveBox2 IVTech), allowing static dynamic conditions through peristaltic pump. The VC was stimulated incubation calcifying medium for 7 days. A BMP Real-Time PCR performed at end each experiment. Results: In monocultures, BMP-2 expression increased calcified (p = 0.01) conditions. BMP-4 receptors expressed all experimental settings, increasing mainly flow co-cultures, we observed marked increase BMPR-1a 0.04 p 0.01, respectively), BMPR-2 0.001) setting mostly Conclusions: BMP-2/4 suggests these genes might promote towards osteogenic-like phenotype, data also supported rise both BMPR-2. Thus, our findings provide insights into which modulates activation mimicking vivo VC’s mechanical characteristics.

Language: Английский

Citations

2