Regulatory T cell-associated gene signature correlates with prognostic risk and immune infiltration in patients with breast cancer DOI Open Access
Jie Wu,

Gaiping Zhao,

Yan Cai

et al.

Translational Cancer Research, Journal Year: 2024, Volume and Issue: 13(12), P. 6766 - 6781

Published: Dec. 1, 2024

Regulatory T cells (Tregs) play a pivotal role in the development, prognosis, and treatment of breast cancer. This study aimed to develop Treg-associated gene signature that contributes predict prognosis therapy benefits genes were screened based on single-cell RNA-sequencing (RNA-seq) TISCH2 database bulk RNA-seq The Cancer Genome Atlas (TCGA) database. was identified via survival analysis, univariate cox, least absolute shrinkage selection operator (LASSO) multivariable Cox regression analyses. Immune status assessed using single-sample set enrichment analysis (ssGSEA) Estimation STromal MAlignant Tumor tissues Expression data (ESTIMATE) algorithms. Drug sensitivity estimated pRRophetic. Gene (GSEA) conducted explore changed pathways. A total 169 as genes, close interactions existed among these genes. Kaplan-Meier (KM) cox revealed 29 prognostic (all P<0.05), finally six-gene including TBC1D4, PMAIP1, IFNG, LEF1, MZB1 EZR by LASSO Cox. Based this signature, patients high-risk group exhibited worse probability than those low-risk TCGA training dataset (P<0.001). Additionally, showed moderate predictive power for 1-, 3- 5-year cancer both [area under curve (AUC) =0.705, 0.678 0.668, respectively]. Similar also observed validation datasets. Risk scores significantly differed between subgroups divided clinicopathologic features, especially molecular subtypes. Patients high- groups significant differences infiltration abundance multiple types immune (such as, activated B cells/CD8+ cells/CD4+ cells), stromal P<0.05). Moreover, 83 chemotherapeutic drugs such lapatinib, methotrexate, gefitinib two risk P<0.001). is first cancer, which could help identify who might be benefit from immunotherapy and/or chemotherapy.

Language: Английский

LINC01089 in cancer: multifunctional roles and therapeutic implications DOI Creative Commons

Qiang Yi,

Gangfeng Zhu,

Xinting Ouyang

et al.

Journal of Translational Medicine, Journal Year: 2024, Volume and Issue: 22(1)

Published: Sept. 27, 2024

Language: Английский

Citations

5
Latest Therapeutical Approaches for Triple-Negative Breast Cancer: From Preclinical to Clinical Research DOI Open Access
Mariona Pont, Marta Marqués, Anabel Sorolla

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(24), P. 13518 - 13518

Published: Dec. 17, 2024

Triple-negative breast cancer (TNBC) represents roughly one-sixth of all patients, but accounts for 30–40% deaths. Due to the lack typical biomarkers exploited clinically cancer, it remains very difficult treat. Moreover, its intrinsic high heterogeneity and proneness become resistant drugs administered makes treatment management challenging oncologists. Herein, we outline different therapies used currently TNBC list ongoing clinical trials provide an overview most recent therapeutic landscape. In addition, highlight emerging in preclinical stage that hold promise, such as epigenetic modulators, CRISPR, miniproteins, radioconjugates, vaccines, PROTACs. navigate through existing limitations challenges which hamper development new more effective treatments TNBC. Lastly, point directions may revolutionize future therapy

Language: Английский

Citations

4
Dual Functions of Androgen Receptor Overexpression in Triple-Negative Breast Cancer: A Complex Prognostic Marker DOI Creative Commons
Umay Kiraz, Emma Rewcastle,

Silja Kavlie Fykse

et al.

Bioengineering, Journal Year: 2025, Volume and Issue: 12(1), P. 54 - 54

Published: Jan. 10, 2025

A subset of triple-negative breast cancer (TNBC) expresses the androgen receptor (AR), but thresholds for AR positivity and its clinical significance vary. We hypothesize that objective assessment outperforms subjective methods, high negatively impacts prognosis. In a population-based TNBC cohort (n = 198) with long follow-up (4–383 months), expression was evaluated via scoring (AR-Manual) automated digital image analysis (AR-DIA). 10% cut-off value AR-DIA strongest negative prognostic threshold distant metastases (p 0.008). High correlated lower grade 0.014), proliferation 0.004) also larger tumors 0.047), metastasis 0.052), lymph node (LN) < 0.001), highlighting dual roles. Multivariate revealed interaction between LN status 0.001) as factor, followed by fibrotic focus (FF; p 0.009), mitotic activity index (MAI; 0.018), stromal tumor-infiltrating lymphocytes (sTILs; 0.041). had no additional in favorable subgroups significant unfavorable subgroups. patients characteristics, ACT did not improve survival, may benefit from AR-targeted therapy. Overall, DIA method provides reproducibility, (≥10%) shows opposing survival effects different subgroups, evaluation is crucial prognosis therapies.

Language: Английский

Citations

0
Comparative analysis of the genomic and expression profiles of ANLN and KDR as prognostic markers in breast Cancer DOI Creative Commons
Aamir Mehmood,

Rongpei Li,

Aman Chandra Kaushik

et al.

In Silico Pharmacology, Journal Year: 2025, Volume and Issue: 13(1)

Published: Jan. 15, 2025

Language: Английский

Citations

0
DNA methyltransferase 3A: A prognostic biomarker and potential target for immunotherapy in gastric cancer DOI Creative Commons

Zhou Wei,

Zhenzhen Kou, Yun Luo

et al.

Medicine, Journal Year: 2025, Volume and Issue: 104(7), P. e41578 - e41578

Published: Feb. 14, 2025

DNA methyltransferase 3A (DNMT3A) has been associated with the occurrence or progression of various tumors, including gastric cancer. However, role DNMT3A in efficacy immune-cell infiltration tumor microenvironment and immunotherapy cancer remains less explored. expression level was analyzed using TIMER 2.0, Sangerbox 3.0, The Cancer Genome Atlas database further verified by immunohistochemical staining RT-qPCR. UALCAN, chi-square test, Kaplan–Meier plotter databases were performed to assess correlation clinicopathological characteristics prognosis. GeneMANIA database, STRING R package used construct a co-expression gene network. Gene set enrichment analysis identify signaling pathways related expression. correlations between immune infiltrates investigated Plotter, package, TISIDB databases. immunomodulators Immune cell Proportion Score. association mutational burden (TMB), microsatellite instability, dryness evaluated TMB function 2.0. Finally, biological cells assessed CCK-8, cloning formation, transwell assay. remarkably upregulated high poor clinical features survival patients Moreover, analyses showed that its genes involved promoted influencing microenvironment. significantly tumor-infiltrating cells, immunomodulators, TMB, checkpoints knockdown reduced proliferation migration cells. Our findings highlight potential as prognosis biomarker an immunotherapeutic target for

Language: Английский

Citations

0
Unravelling the role of ubiquitin-specific proteases in breast carcinoma: insights into tumour progression and immune microenvironment modulation DOI Creative Commons
Huiyuan Yang,

Tingting Sun,

Zhenni Sun

et al.

World Journal of Surgical Oncology, Journal Year: 2025, Volume and Issue: 23(1)

Published: Feb. 20, 2025

Breast cancer is a prevalent malignancy worldwide, and its treatment has increasingly shifted towards precision medicine, with immunotherapy emerging as key therapeutic strategy. Deubiquitination, an essential epigenetic modification, regulated by deubiquitinating enzymes (DUBs) plays critical role in immune function tumor progression. Ubiquitin-specific proteases (USPs), prominent subgroup of DUBs, are involved regulating cell functions, antigen processing, T development the context breast cancer. Certain USPs also modulate differentiation cells, such myeloid-derived suppressor cells (MDSCs) regulatory (Tregs), within microenvironment. Furthermore, several influence expression PD-L1, thus affecting efficacy checkpoint inhibitors. The overexpression may promote evasion, contributing to resistance. This review elucidates modulating microenvironment responses Additionally, it discusses effective strategies for combining USP inhibitors other agents enhance outcomes. Therefore, targeting presents potential overcome drug resistance, offering more strategy patients.

Language: Английский

Citations

0
Unveiling the multifaceted roles of long non-coding RNA CTBP1-DT in human diseases: Special attention to its microprotein-encoding potential DOI

Jingjie Yang

Pathology - Research and Practice, Journal Year: 2025, Volume and Issue: 268, P. 155870 - 155870

Published: Feb. 26, 2025

Language: Английский

Citations

0
Evaluation of Efficacy of Serum IL-12 and IFN-<i>γ</i> in Neoadjuvant Chemotherapy for Breast Cancer DOI

祖格 刘

Advances in Clinical Medicine, Journal Year: 2025, Volume and Issue: 15(02), P. 1839 - 1849

Published: Jan. 1, 2025

Language: Английский

Citations

0
Anti-Tumor Potential of Frankincense Essential Oil and Its Nano-Formulation in Breast Cancer: An In Vivo and In Vitro Study DOI Creative Commons

Nouran Mohamed,

Hisham Ismail, Ghada M. Nasr

et al.

Pharmaceutics, Journal Year: 2025, Volume and Issue: 17(4), P. 426 - 426

Published: March 27, 2025

Background/Objective: Breast cancer remains the most common malignancy among women worldwide, contributing to high morbidity and mortality rates. Many anti-cancer drugs have been derived from medicinal plants, frankincense Boswellia carterii is notable for its anti-inflammatory, anti-neoplastic, anti-carcinogenic properties. Using gas chromatography/mass spectrometry (GC/MS), 48 components were identified in B. essential oil, major constituent was α-pinene (35.81%). Method: In this study, we investigated anti-tumor effects of oil (FEO) nano-formulation with chitosan (FEO-CSNPs) using vitro breast models (MCF-7, MDA-MB-231, 4T1 cells) vivo mouse mammary carcinoma (4T1) (Balb/c). Results: The results showed significant reductions cell viability. At 10 μg/mL, FEO highest reduction C-166 cells, while at 100 exhibited a stronger cytotoxicity MDA-MB-231 cells compared FEO-CSNPs CSNPs. growth arrest S, G2/M, G1/S phases MCF-7, lines (36.91%, 23.12%, 33.58%), addition increased apoptosis rates (33.04%, 36.39%, 42.19%). wound healing assays revealed decreased migratory ability treated cells. experiments balb/c mice demonstrated tumor volume, histopathological analysis confirming extensive necrosis. Moreover, antioxidant arginase activity. gene expression via qPCR indicated upregulation suppressor genes downregulation oncogenes. Conclusions: These findings suggest that nano-formulation, particularly form as an oral formulation, display enhanced efficacy, warranting further preclinical clinical research develop innovative treatment strategies.

Language: Английский

Citations

0
The role of ferroptosis in breast cancer: tumor progression, immune microenvironment interactions and therapeutic interventions DOI
Yiping Wang,

Chuanyun Tang,

Keqin Wang

et al.

European Journal of Pharmacology, Journal Year: 2025, Volume and Issue: 996, P. 177561 - 177561

Published: March 28, 2025

Language: Английский

Citations

0