Screening and Interaction Analysis of Shark-Derived Nanobodies against Crayfish Major Allergen Pro c 2 DOI
Yang Yang,

Xinrong He,

Si‐Yang He

et al.

Journal of Agricultural and Food Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: April 21, 2025

Pro c 2 (arginine kinase) is a major allergen in crayfish (Procambarus clarkii). Shark-derived variable domains of new antigen receptors (VNARs) have advantages developing detection and immunotherapy. This study constructed VNAR domain library from Chiloscyllium plagiosum immunized with 2. Three VNARs (VNAR-11, VNAR-20, VNAR-29) against obtained by screening the were expressed HEK293F cells, fusing immunoglobulin (Ig) G1 Fc fragment (VNAR-Fc-11, VNAR-Fc-29, VNAR-Fc-20). The VNAR-Fc fusions bound to an affinity KD ranging 0.2131 ∼ 465.3 μM, ability inhibit patients' IgE binding VNAR-20 VNAR-29 displayed more stable during molecular dynamics simulation. sites are distributed conserved epitopes arginine kinase. These achievements indicate application potential allergy therapeutics.

Language: Английский

Screening and Interaction Analysis of Shark-Derived Nanobodies against Crayfish Major Allergen Pro c 2 DOI
Yang Yang,

Xinrong He,

Si‐Yang He

et al.

Journal of Agricultural and Food Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: April 21, 2025

Pro c 2 (arginine kinase) is a major allergen in crayfish (Procambarus clarkii). Shark-derived variable domains of new antigen receptors (VNARs) have advantages developing detection and immunotherapy. This study constructed VNAR domain library from Chiloscyllium plagiosum immunized with 2. Three VNARs (VNAR-11, VNAR-20, VNAR-29) against obtained by screening the were expressed HEK293F cells, fusing immunoglobulin (Ig) G1 Fc fragment (VNAR-Fc-11, VNAR-Fc-29, VNAR-Fc-20). The VNAR-Fc fusions bound to an affinity KD ranging 0.2131 ∼ 465.3 μM, ability inhibit patients' IgE binding VNAR-20 VNAR-29 displayed more stable during molecular dynamics simulation. sites are distributed conserved epitopes arginine kinase. These achievements indicate application potential allergy therapeutics.

Language: Английский

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