Ultrasensitive Peptide-Based Electrochemical Biosensor for Universal Diagnostic of Dengue

Biosensors, Journal Year: 2025, Volume and Issue: 15(4), P. 236 - 236

Published: April 8, 2025

Dengue is a neglected disease mainly affecting tropical and subtropical countries. The diagnosis of dengue fever still problem since most it made from whole or recombinant DENV proteins, which present cross-reactions with other members the Flavivirus family. Therefore, there huge demand for new diagnostic methods that provide rapid, low-cost, easy-to-use confirmation. Thus, in this study, we developed an affordable electrochemical biosensor rapidly detecting immunoglobulin G (IgG) serological antibodies sera DENV-infected patients. An identified linear B-cell epitope (DENV/18) specific 1–4 serotypes recognized by IgG patient was selected as target molecule after microarray peptides using SPOT-synthesis methodology. After chemical synthesis, DENV/18-peptide immobilized on surface working electrode commercially available screen-printed gold (SPGE). capture DENV-specific allowed formation immunocomplex measured cyclic voltammetry (CV) differential pulse (DPV) potassium ferrocyanide/ferricyanide ([Fe(CN)6]3−/4−) probe. evaluation biosensor’s performance showed detection limit 100 µg mL−1 synthetic 1.21 ng CV 0.43 DPV human serum, sensitivity 7.21 µA 8.79 DPV. differentiation infected uninfected individuals possible even at high dilution factor reduced required sample volumes to few microliters. final device proved suitable diagnosing analyzing real serum samples, results good agreement molecular biology diagnostics. flexibility conjugate antigenic SPEs suggests technology could be adapted diagnose pathogens.

Language: Английский

Exploring the Contrasts and Similarities of Dengue and SARS-CoV-2 Infections During the COVID-19 Era

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(21), P. 11624 - 11624

Published: Oct. 29, 2024

Extensive research has been conducted on the SARS-CoV-2 virus in association with various infectious diseases to understand pathophysiology of infection and potential co-infections. In tropical countries, exposure local viruses may alter course coinfection. Notably, only a portion antibodies produced against proteins demonstrate neutralizing properties, immune response following natural tends be temporary. contrast, long-lasting IgG are common after dengue infections. cases where preexisting from an initial bind different serotype during subsequent infection, there is for antibody-dependent enhancement (ADE) formation complexes associated disease severity. Both infections can result immunodeficiency. Viral both interfere host's IFN-I signaling. Additionally, cytokine storm occur viral impairing proper response, autoantibodies wide array appear convalescence. Most reported typically short-lived. Vaccines affecting enhancing clearance. A comprehensive analysis pathogenicity revisited prevent severity, mortality.

Language: Английский