Inflammatory Transformation of Skin Basal Cells as a Key Driver of Cutaneous Aging DOI Open Access
Shupeng Liu,

Sheng Lu,

Zhiping P. Pang

et al.

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(6), P. 2617 - 2617

Published: March 14, 2025

This study comprehensively investigated keratinocyte subpopulation heterogeneity and developmental trajectories during skin aging using single-cell sequencing, transcriptomics, facial aging-related genome-wide association studies (GWAS) data. We identified three major subpopulations: basal cells (BCs), spinous (SCs), IFI27+ keratinocytes. Single-cell pseudotime analysis revealed that can differentiate along two distinct paths: toward differentiation or the inflammatory state. With aging, proportion of significantly increased, displaying more active immunomodulatory signals. Through cell–cell communication analysis, we found signaling pathways, including NOTCH, PTPR, PERIOSTIN, exhibited characteristics different branches. Integration GWAS data significant loci on chromosomes 2, 3, 6, 9 were spatially correlated with key biological pathways (including antigen processing, oxidative stress, apoptosis). These findings reveal complex cellular molecular mechanisms underlying offering potential targets for novel diagnostic approaches therapeutic interventions.

Language: Английский

Inflammatory Transformation of Skin Basal Cells as a Key Driver of Cutaneous Aging DOI Open Access
Shupeng Liu,

Sheng Lu,

Zhiping P. Pang

et al.

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(6), P. 2617 - 2617

Published: March 14, 2025

This study comprehensively investigated keratinocyte subpopulation heterogeneity and developmental trajectories during skin aging using single-cell sequencing, transcriptomics, facial aging-related genome-wide association studies (GWAS) data. We identified three major subpopulations: basal cells (BCs), spinous (SCs), IFI27+ keratinocytes. Single-cell pseudotime analysis revealed that can differentiate along two distinct paths: toward differentiation or the inflammatory state. With aging, proportion of significantly increased, displaying more active immunomodulatory signals. Through cell–cell communication analysis, we found signaling pathways, including NOTCH, PTPR, PERIOSTIN, exhibited characteristics different branches. Integration GWAS data significant loci on chromosomes 2, 3, 6, 9 were spatially correlated with key biological pathways (including antigen processing, oxidative stress, apoptosis). These findings reveal complex cellular molecular mechanisms underlying offering potential targets for novel diagnostic approaches therapeutic interventions.

Language: Английский

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