Springer eBooks, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 40
Published: Jan. 1, 2025
Language: Английский
Biochemical and Biophysical Research Communications, Journal Year: 2025, Volume and Issue: 750, P. 151424 - 151424
Published: Jan. 30, 2025
Language: Английский
Citations
0
Natural Product Communications, Journal Year: 2025, Volume and Issue: 20(3)
Published: March 1, 2025
Background Methicillin-resistant Staphylococcus aureus (MRSA) is a major global health threat, causing infections that are difficult to treat due resistance standard antibiotics. The high mortality rates and limited treatment options for MRSA underscore the urgent need novel therapeutic agents. Chelerythrine, natural alkaloid, has shown potent antimicrobial activity against MRSA. However, molecular mechanisms underlying its antibacterial effects, particularly impact on bacterial proteomes, remain poorly understood. This study investigates chelerythrine's efficacy time-dependent effects proteome uncover key metabolic pathways targeted by this compound. Methods minimum inhibitory concentration (MIC) of chelerythrine was determined using microdilution method. Proteomic changes were analyzed label-free quantitative mass spectrometry at 30, 60, 120 min post-exposure. Fuzzy C-means (FCM) clustering employed identify protein expression patterns. Results Chelerythrine exhibited with MIC 19.54 mg/L MRSA, dose-dependent growth inhibition. analysis identified 1037 proteins, 447, 340, 265 proteins showing significant min, respectively. FCM revealed three clusters, Cluster 3 (36 proteins) specific downregulation under treatment. Gene Ontology (GO) KEGG analyses indicated enrichment in pathways, glycolysis, tricarboxylic acid cycle, arginine catabolism, highlighting effect energy production. Conclusion disrupts those involved production amino metabolism, suggesting potential as an agent.
Language: Английский
Citations
0
Microorganisms, Journal Year: 2025, Volume and Issue: 13(4), P. 953 - 953
Published: April 21, 2025
Xanthomonas oryzae pv. (Xoo) is a biotrophic bacterial pathogen, which causes devastating blight disease worldwide. In this study, we thoroughly investigated the antimicrobial effect of plant-derived extract chelerythrine against and elucidated its mechanism. Chelerythrine quaternary ammonium alkaloid with 2,3,7,8-tetrasubstituted phenanthridine structure, extracted from plants, such as whole plant Chelidonium majus, roots, stems, leaves Macleaya cordata. We found that significantly inhibited growth Xoo at concentration 1.25 μg/mL. Further experiments revealed interfered division reproduction bacterium, leading to filamentous growth. Additionally, it increased permeability cell membranes effectively decreased pathogenicity Xoo, including inhibition extracellular polysaccharide production, cellulase secretion, biofilm formation. induced accumulation reactive oxygen species in triggering oxidative stress. The result showed formation Z-ring synthesis pyrimidine purine nucleotides, DNA damage repair, peptidoglycan lipid-like A, thus interfering membrane permeability, inhibiting carbohydrate metabolism phosphorylation sugars, reducing pathogenicity, ultimately destruction or lysis cells. Altogether, our results suggest on exhibits multi-target properties. effective inhibitory low. These findings provide crucial theoretical basis guidance for development novel efficient compounds.
Language: Английский
Citations
0
Naunyn-Schmiedeberg s Archives of Pharmacology, Journal Year: 2025, Volume and Issue: unknown
Published: April 22, 2025
Language: Английский
Citations
0
International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(9), P. 4030 - 4030
Published: April 24, 2025
The global healthcare system is increasingly challenged by the rising prevalence of multidrug-resistant bacteria and limited therapeutic options for related infections. Efflux-mediated antibiotic resistance represents a significant obstacle, primarily due to absence drugs specifically designed target bacterial efflux pumps. Recent research has identified polyphenols, broad class plant-derived organic compounds, as potential inhibitors pump activity. This review consolidates data on inhibitory properties eight widely distributed polyphenols: curcumin, quercetin, luteolin, tannic acid, naringenin, epigallocatechin-3-gallate, ellagic resveratrol. These compounds have demonstrated capacity inhibit pumps, either through direct interference with protein function or downregulating expression genes encoding subunits. Importantly, several polyphenols exhibit synergistic interactions antibiotics, including colistin, ciprofloxacin, tetracycline. For instance, quercetin shown potency comparable that established such verapamil reserpine. findings suggest represent promising candidates development novel inhibitors. However, further required validate their efficacy safety facilitate translation into clinical applications combating resistance.
Language: Английский
Citations
0
Springer eBooks, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 40
Published: Jan. 1, 2025
Language: Английский
Citations
0