Multi-omic analysis on the molecular mechanisms of rapid growth in ‘Deqin’ alfalfa after space mutagenesis DOI Creative Commons

Qian Kuang,

Chenggang He, Heping Huang

et al.

BMC Plant Biology, Journal Year: 2025, Volume and Issue: 25(1)

Published: Jan. 9, 2025

Space-induced plant mutagenesis, driven by cosmic radiation, offers a promising approach for the selective breeding of new varieties. By leveraging unique environment outer space, we successfully induced mutagenesis in 'Deqin' alfalfa and obtained fast-growing mutant. However, molecular mechanisms underlying its rapid growth remain poorly unexplored. Comparative analyses transcriptomics, proteomics, hormone profiles were conducted root, stem, leaf tissues both mutant non-mutagenic materials. Targeted showed notable increases levels 3-indolebutyric, indole-3-acetic, 3-indolepropionic acids mutant, with percentage 33.55%, 32.49%, 30.39%, respectively. Zeatin-riboside dihydrozeatin riboside increased 164.92% 25.86%, while giberellin (GA) 7, GA3, GA1 219.52%, 68.74%, 40.98%. Non-mutagenic materials sprayed exogenous 3-indolebutyric acid, zeatin-riboside, GA7 exhibited significant acceleration. Transcriptomics identified 49,095 annotated genes, 2,009, 1,889, 1,760 upregulated 2,082, 2,035, 1,499 downregulated leaves, stems, roots, Twenty-two genes related to biosynthesis alterations. Screening through weighted correlation network analysis revealed ten candidate four which associated photosynthesis starch sucrose metabolism. Integrated targeted metabolomics transcriptomics indicated that signal transduction played crucial role. Proteomics 479 differentially accumulated proteins, 174 305 downregulated. proteomics photosynthesis, metabolism, carbon fixation photosynthetic organisms promoted mutants. integrating multi-omics data, elucidated synergistic effects pathways such as tryptophan metabolism on This study demonstrated significance hormones mutants key metabolic pathways. Our findings provide valuable information genetic improvement varieties serve reference achieving other plants.

Language: Английский

Multi-omic analysis on the molecular mechanisms of rapid growth in ‘Deqin’ alfalfa after space mutagenesis DOI Creative Commons

Qian Kuang,

Chenggang He, Heping Huang

et al.

BMC Plant Biology, Journal Year: 2025, Volume and Issue: 25(1)

Published: Jan. 9, 2025

Space-induced plant mutagenesis, driven by cosmic radiation, offers a promising approach for the selective breeding of new varieties. By leveraging unique environment outer space, we successfully induced mutagenesis in 'Deqin' alfalfa and obtained fast-growing mutant. However, molecular mechanisms underlying its rapid growth remain poorly unexplored. Comparative analyses transcriptomics, proteomics, hormone profiles were conducted root, stem, leaf tissues both mutant non-mutagenic materials. Targeted showed notable increases levels 3-indolebutyric, indole-3-acetic, 3-indolepropionic acids mutant, with percentage 33.55%, 32.49%, 30.39%, respectively. Zeatin-riboside dihydrozeatin riboside increased 164.92% 25.86%, while giberellin (GA) 7, GA3, GA1 219.52%, 68.74%, 40.98%. Non-mutagenic materials sprayed exogenous 3-indolebutyric acid, zeatin-riboside, GA7 exhibited significant acceleration. Transcriptomics identified 49,095 annotated genes, 2,009, 1,889, 1,760 upregulated 2,082, 2,035, 1,499 downregulated leaves, stems, roots, Twenty-two genes related to biosynthesis alterations. Screening through weighted correlation network analysis revealed ten candidate four which associated photosynthesis starch sucrose metabolism. Integrated targeted metabolomics transcriptomics indicated that signal transduction played crucial role. Proteomics 479 differentially accumulated proteins, 174 305 downregulated. proteomics photosynthesis, metabolism, carbon fixation photosynthetic organisms promoted mutants. integrating multi-omics data, elucidated synergistic effects pathways such as tryptophan metabolism on This study demonstrated significance hormones mutants key metabolic pathways. Our findings provide valuable information genetic improvement varieties serve reference achieving other plants.

Language: Английский

Citations

0