Effect of agarose/gelatin gel addition on the pro-angiogenic potential of polyhydroxybutyrate/chitosan scaffolds DOI Creative Commons
Mária Giretová, Ľubomír Medvecký, Zuzana Demčišáková

et al.

Frontiers in Cell and Developmental Biology, Journal Year: 2025, Volume and Issue: 12

Published: Jan. 21, 2025

The aim of this paper was to evaluate the effect gel addition biopolymeric scaffolds on pro-angiogenic and basic material characteristics final composite for use in regenerative medicine. studied scaffold consisted natural biopolymers: polyhydroxybutyrate, chitosan, agarose, gelatin. characterized by high macroporosity (90%) wide pore size distribution. As is known, a critical factor cell ingrowth grafts after implantation body angiogenic development creation new vessels. After 9 days cultivation culture medium, retained its physicochemical properties without any tendency disintegration. polymeric gels improved mechanical stability composite. In vitro cytotoxicity testing showed good adherence seeded L929 fibroblasts strong cells into macropores. No sign identified both MTS assay live/dead staining. quail chorioallantoic membrane (CAM) assay—as an alternative vivo assays—revealed suitable formation blood Moreover, upregulation gene expression responsible activation cascade clearly demonstrated positive prepared composites angiogenesis as essential part tissue regeneration process itself.

Language: Английский

Effect of agarose/gelatin gel addition on the pro-angiogenic potential of polyhydroxybutyrate/chitosan scaffolds DOI Creative Commons
Mária Giretová, Ľubomír Medvecký, Zuzana Demčišáková

et al.

Frontiers in Cell and Developmental Biology, Journal Year: 2025, Volume and Issue: 12

Published: Jan. 21, 2025

The aim of this paper was to evaluate the effect gel addition biopolymeric scaffolds on pro-angiogenic and basic material characteristics final composite for use in regenerative medicine. studied scaffold consisted natural biopolymers: polyhydroxybutyrate, chitosan, agarose, gelatin. characterized by high macroporosity (90%) wide pore size distribution. As is known, a critical factor cell ingrowth grafts after implantation body angiogenic development creation new vessels. After 9 days cultivation culture medium, retained its physicochemical properties without any tendency disintegration. polymeric gels improved mechanical stability composite. In vitro cytotoxicity testing showed good adherence seeded L929 fibroblasts strong cells into macropores. No sign identified both MTS assay live/dead staining. quail chorioallantoic membrane (CAM) assay—as an alternative vivo assays—revealed suitable formation blood Moreover, upregulation gene expression responsible activation cascade clearly demonstrated positive prepared composites angiogenesis as essential part tissue regeneration process itself.

Language: Английский

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