Unveiling the molecular mechanisms of stigmasterol on diabetic retinopathy: BNM framework construction and experimental validation

Frontiers in Medicine, Journal Year: 2025, Volume and Issue: 12

Published: May 9, 2025

Background Diabetic retinopathy (DR), one of the most common complications diabetes, severely impacts patients’ quality life. The combined use traditional Chinese medicines Astragalus, Fructus ligustris, and Cornus officinalis has yielded considerable therapeutic effects in clinical DR treatment. Methods In this study, a multimodule framework (BNM) encompassing bioinformatics, network pharmacology, machine learning (ML) based on molecular fingerprints was innovatively developed to thoroughly investigate mechanisms medicine treating DR. Results A total 40 active components 12 core targets were identified. Enrichment analysis identified key pathways such as VEGF signaling pathway, TNF pathway HIF-1 pathway. Prediction models using targets, PPARG, constructed from GEO database validated via immune infiltration docking, revealing that PPARG may be potential target for Moreover, component medicine, stigmasterol, ML model fingerprints. vivo experiments demonstrated stigmasterol can regulate glucose lipid metabolism, improve systemic inflammatory levels, ameliorate ocular vascular changes by modulating expression PPARG. Conclusion BNM suggests an important treatment DR, with its mechanism potentially related VEGF/VEGFR

Language: Английский

Modulation of Lipogenesis by Tetracarpidium conophorum Nuts via SREBP-1/ACCA-1/FASN Inhibition in Monosodium-Glutamate-Induced Obesity in Rats

Natural Product Communications, Journal Year: 2025, Volume and Issue: 20(5)

Published: May 1, 2025

Objectives This study aimed to investigate the modulatory effects of Tetracarpidium conophorum (African walnut) nuts on lipogenesis in adipocytes and hepatocytes by targeting SREBP-1/ACCA-1/FASN signaling axis, which plays a pivotal role obesity-associated lipid metabolism. Methods Obesity was induced rats over 36 weeks, after groups were treated with whole extract (WE), ethyl acetate fraction (EA), residue (RES) T. nuts, or Orlistat (5.14 mg/kg). Post-treatment, hepatic adipose tissues collected analyzed for gene expression levels SREBP-1, ACCA-1, FASN using PCR. Additionally, GC-MS-identified compounds from nut extracts subjected molecular docking against target proteins, their drug-likeness ADMET profiles evaluated. Results Treatment WE, EA, RES significantly downregulated SREBP-1 both compared obese control. ACCA-1 tissue not affected, but overexpression attenuated WE (p < 0.05), EA 0.01), 0.01). Molecular revealed strong binding affinities stable interactions between bioactive proteins. Notably, 6-Isopropenyl-4,8a-dimethyl-4a,5,6,7,8,8a-hexahydro-1H-naphthalen-2-one exhibited strongest (−7.7 kcal/mol), (−7.2 (−7.8 outperforming (−5.9 −6.2 kcal/mol). Drug-likeness assessments predictions supported therapeutic potential compounds. Conclusion demonstrate significant anti-obesity through inhibition via downregulation pathway, specific showing promising pharmacokinetic properties.

Language: Английский

Camellia sinensis L. (Green Tea): Bioactive Compounds and Pharmacological Properties – A Comprehensive Review

Hop and Medicinal Plants, Journal Year: 2024, Volume and Issue: 32(1-2), P. 102 - 118

Published: Dec. 26, 2024

Medicinal plants have become increasingly preferred over drugs due to the sense of well-being they promote, thanks their rich content in bioactive compounds with many health benefits, as well lack adverse effects compared pharmaceuticals. At same time, can relieve symptoms some conditions and also contribute prevention various chronic diseases, including obesity, diabetes, cancer. Camellia sinensis L., known plant from which green, black, oolong teas are obtained, other varieties, is one most famous medicinal plants, bringing a multitude benefits human body, mainly through its leaves, largest proportion being represented by catechins, type polyphenol recognized for strong antioxidant properties. EGCG (epigallocatechin gallate), important abundant catechin, ability neutralize free radicals reduce oxidative stress.Although less studied, flowers this found along unique but others multiple therapeutic Research indicates that regular consumption green tea (Camellia L.) significantly enhances life quality promotes longevity lowering risk cardiovascular diseases certain types cancer, colorectal gastric cancers.

Language: Английский