Effects of different Lys/Met ratios on the antioxidant capacity, tissue morphology, and fatty acid composition of subcutaneous fat in Tibetan sheep on low-protein diets: a lipidomic analysis

Frontiers in Veterinary Science, Journal Year: 2025, Volume and Issue: 11

Published: Jan. 30, 2025

Introduction This study employed lipidomics to investigate the effects of varying lysine (Lys)- to-methionine (Met) ratios on antioxidant capacity, tissue morphology, and fatty acid composition subcutaneous fat in Tibetan sheep fed a low-protein diet. Methods Ninety healthy male similar body weight were randomly allocated into three groups. These diet containing Lys/Met 1:1, 2:1, 3:1. Ultra-High Performance Liquid Chromatography–tandem Mass Spectrometry (UHPLC–MS/MS) was explore changes various lipid subclasses adipose tissue. The expression genes associated with adipogenesis, metabolism also examined. Results results indicated that 1:1 group exhibited significantly higher capacity (glutathione peroxidase, GSH-Px), more orderly adipocyte arrangement, uniform cell size, general increase unsaturated levels. Additionally, several molecules phenotype (Antioxidant index content) identified, namely, DG(38:3e) + Na, PE(17:1_22:2)-H, PI(17:0_20:3)-H, TG(33:0e) NH4, Cer(d14:0_17:1) H, CL(81:13)-2H. Furthermore, findings showed upregulation PPARγ, FASN, FAD4, CPT1A , GPX4 can enhance differentiation accumulation, thereby improving metabolic function via regulation oxidative defense mechanisms. Discussion In summary, this provides theoretical foundation for optimizing precision feeding strategies sheep, offering crucial data support enhancements production efficiency meat quality.

Language: Английский

Review of Precision Medicine and Diagnosis of Neonatal Illness

Diagnostics, Journal Year: 2025, Volume and Issue: 15(4), P. 478 - 478

Published: Feb. 16, 2025

Background/Objectives: Precision medicine is a state-of-the-art tactic that tailors information about people's genes, environment, and lifestyle to aid the prevention, diagnosis, treatment of various diseases provide an overview currently available knowledge applicability precision in diagnosis different cases admitted NICU, such as encephalopathies, respiratory distress syndrome prematurity, hemodynamic instability, acute kidney injury, sepsis, hyperbilirubinemia. Methods: The authors searched databases, PubMed Central, for terms neonatal "precision medicine", "personalized "genomics", "metabolomics", all related illness. studies were collected. Results: review highlights diagnostic approach serves implement NICU monitoring, treatment. Conclusions: In this review, we projected several approaches identification health problems among sick neonates with complex illnesses NICU; some are noninvasive ordinary healthcare settings, while others invasive or not feasible still ongoing research machine learning algorithms. Future needed wide implementation artificial intelligence tools illnesses.

Language: Английский

Assessment of the effect of hypothermia after simulated hypoxic ischemic encephalopathy based on blood metabolome

Bulletin of Russian State Medical University, Journal Year: 2024, Volume and Issue: 2024(6)

Published: Dec. 1, 2024

Hypoxic ischemic encephalopathy (HIE) is a severe neonatal condition causing various neurological disorders and one of the main causes mortality among full-term babies. Therapeutic hypothermia (TH), i.e. newborn’s body temperature decrease that significantly reduces risk fatality contributes to improvement long-term outcomes in infants with HIE key treatment method for moderate-to-severe HIE. However, timely diagnosis disease severity determination are crucial this be used, has number limitations requirements. Assessment mechanism underlying effects TH search major metabolic pathways potential targets therapy relevant. The study aimed assess metabolome dried blood spots by HPLC-MS, since it least invasive patients test markers most active likely mediate its positive effects. As result, alterations class phosphoglycerolipids were found, which suggests an important role endocannabinoid metabolism protection against Furthermore, ubiquinone, certain fatty acids, bile acids altered. targeted quantitative studies these metabolites will make possible optimize based on identified.

Language: Английский