Production, Purification, and Characterization of Extracellular Lipases from Hyphopichia wangnamkhiaoensis and Yarrowia deformans DOI Creative Commons

Misael Romo-Silva,

Emanuel Osmar Flores-Camargo,

Griselda Ma. Chávez-Camarillo

et al.

Fermentation, Journal Year: 2024, Volume and Issue: 10(12), P. 595 - 595

Published: Nov. 21, 2024

The efficient production of microbial lipases from organic wastes has garnered great interest because the diverse and potential biotechnological applications these enzymes. However, extracellular novel yeast strains Hyphopichia wangnamkhiaoensis Yarrowia deformans remain uncharacterized. Thus, this study aimed to investigate characteristics both yeasts. Lipases H. Y. were purified biochemically characterized, their was measured in batch cultures with olive oil (reference), waste cooking oil, glycerol as substrates. had molecular weights approximately 33 45 kDa, respectively. Their activities on p-nitrophenyl palmitate optimal at pH 8.0 40 °C. Moreover, inhibited by ethylenediaminetetraacetic acid, phenylmethylsulfonyl fluoride, 4-(2-aminoethyl)benzenesulfonyl reactivated Ca2+ Mg2+, indicating that are metalloenzymes serine-type more tolerant hydrophilic solvents than hydrophobic solvents, they followed Michaelis–Menten kinetics. Among various substrates used, yielded highest lipase productivity. These results indicate suitable candidates for production.

Language: Английский

Riboflavin Production by Steady-State Continuous Cultures of Hyphopichia wangnamkhiaoensis in a Bubble Column Bioreactor DOI Creative Commons
Raziel Arturo Jiménez-Nava, Griselda Ma. Chávez-Camarillo, Eliseo Cristiani‐Urbina

et al.

Microorganisms, Journal Year: 2025, Volume and Issue: 13(4), P. 817 - 817

Published: April 3, 2025

Riboflavin is biosynthesized and excreted extracellularly by the novel yeast Hyphopichia wangnamkhiaoensis. The steady-state kinetics of cell growth, substrate consumption, riboflavin production H. wangnamkhiaoensis were studied in a chemostat continuous culture at different dilution rates. unstructured Monod Luedeking–Piret models used to describe production, crucial kinetic parameters estimated. experimental data fitted proposed well. maximum specific growth rate, affinity constant, maintenance energy coefficient, biomass yield values 0.1378 h−1, 0.4166 g glucose L−1, 0.1047 g−1 0.172 glucose, respectively. from volumetric productivities 0.7487 mg 0.5593 L−1 0.6547 estimated growth-associated constant (4.88 biomass) was much higher than non-growth-associated (0.0022 h−1), indicating that predominantly process. offers promising strategy for efficiently sustainably producing using

Language: Английский

Citations

0
Environmental yeasts differentially impact the development and oviposition behavior of the Asian tiger mosquito Aedes albopictus DOI Creative Commons

Simon Malassigné,

Mathieu Laÿs,

Laurent Vallon

et al.

Microbiome, Journal Year: 2025, Volume and Issue: 13(1)

Published: April 16, 2025

While the Asian tiger mosquito (Aedes albopictus), a known vector of many arboviruses, establishes symbiotic associations with environmentally acquired yeasts, their impact on biology remains poorly investigated. To better understand these associations, we hypothesized that waterborne yeasts colonizing larval gut differentially support development based capacity to produce riboflavin or recycle nitrogen waste into proteins by secreting uricase, as B vitamins and amino acids are crucial for development. address this hypothesis, used axenic gnotobiotic insects gauge specific different environmental Ae. albopictus survival. We then evaluated whether observed variations across yeast species could be linked differential uricolytic activities varying quantities in insecta. Finally, given oviposition site selection favors conditions enhance offspring performance, tested promote faster mediate gravid females. Differences times were used. Yeasts like Rhodotorula mucilaginosa Aureobasidium pullulans promoted rapid associated improved Conversely, such Torulaspora delbrueckii Martiniozyma asiatica, which led slower development, produced smaller adults. Notably, R. mucilaginosa, fastest provided high intakes nitrogenous recycling protein synthesis through strong uricolytic-ureolytic activity. Behavioral experiments indicated promoting "attract Our findings highlight set present natural breeding sites can survival enhancing nutritional intake, thereby attracting Variations time likely levels production capacities among species. This study opens new perspectives trophic interactions between mosquitoes mycobiota, emphasizing importance nitrogen-containing molecules essential acids, proteins, mycobiota. Video Abstract.

Language: Английский

Citations

0
Macrogenomic and physicochemical analyses reveal microbial community and functional differences among traditional, commercial, and fortified Xiaoqu DOI

Shaoji Zhang,

Yao Lin, Tianxiang Wu

et al.

Food Bioscience, Journal Year: 2025, Volume and Issue: unknown, P. 106736 - 106736

Published: April 1, 2025

Language: Английский

Citations

0
Production, Purification, and Characterization of Extracellular Lipases from Hyphopichia wangnamkhiaoensis and Yarrowia deformans DOI Creative Commons

Misael Romo-Silva,

Emanuel Osmar Flores-Camargo,

Griselda Ma. Chávez-Camarillo

et al.

Fermentation, Journal Year: 2024, Volume and Issue: 10(12), P. 595 - 595

Published: Nov. 21, 2024

The efficient production of microbial lipases from organic wastes has garnered great interest because the diverse and potential biotechnological applications these enzymes. However, extracellular novel yeast strains Hyphopichia wangnamkhiaoensis Yarrowia deformans remain uncharacterized. Thus, this study aimed to investigate characteristics both yeasts. Lipases H. Y. were purified biochemically characterized, their was measured in batch cultures with olive oil (reference), waste cooking oil, glycerol as substrates. had molecular weights approximately 33 45 kDa, respectively. Their activities on p-nitrophenyl palmitate optimal at pH 8.0 40 °C. Moreover, inhibited by ethylenediaminetetraacetic acid, phenylmethylsulfonyl fluoride, 4-(2-aminoethyl)benzenesulfonyl reactivated Ca2+ Mg2+, indicating that are metalloenzymes serine-type more tolerant hydrophilic solvents than hydrophobic solvents, they followed Michaelis–Menten kinetics. Among various substrates used, yielded highest lipase productivity. These results indicate suitable candidates for production.

Language: Английский

Citations

0