Isolation and Identification of Colletotrichum nymphaeae as a Causal Agent of Leaf Spot on Rhododendron hybridum Ker Gawl and Its Effects on the Ultrastructure of Host Plants DOI Creative Commons
Yajiao Sun, Yao Tian, Jian Liu

et al.

Journal of Fungi, Journal Year: 2025, Volume and Issue: 11(5), P. 392 - 392

Published: May 19, 2025

Rhododendron hybridum Ker Gawl, a widely cultivated horticultural species in China, is highly valued for its ornamental and medicinal properties. However, with the expansion of cultivation, leaf spot disease has become more prevalent, significantly affecting value R. Gawl. In this study, Gawl from Kunming area was selected as experimental material. The tissue isolation method employed study to isolate pathogenic strains. biological characteristics pathogens were determined using mycelial growth rate method. pathogens’ influence on host plant’s ultrastructure investigated transmission electron microscopy (TEM). Colletotrichum nymphaeae identified pathogen implicated development across three regions City through integration morphological traits phylogenetic analyses multiple genes (ITS, ACT, GAPDH, HIS3, CHS1, TUB2). Its most effective at temperature 25 °C. pH light have relatively minor effects mycelium. preferred carbon nitrogen sources mannitol yeast extract, respectively. Additionally, TEM observations revealed significant damage cell structure leaves infected by pathogen. walls dissolved, number chloroplasts decreased markedly, starch granules within largely absent, osmiophilic increased. This first report caused C. nymphaeae. results provide valuable insights future research prevention control disease.

Language: Английский

Adaptive Defense Mechanism During Flowering Period of Rhododendron decorum Revealed by Comparative Transcriptomic Analysis DOI Creative Commons
Weiwei Liu,

Chenghua Yu,

Kaiye Yang

et al.

Plants, Journal Year: 2025, Volume and Issue: 14(4), P. 559 - 559

Published: Feb. 12, 2025

Rhododendron decorum, a widely distributed species in southwestern China, is recognized not only for its significant ornamental value but also as culinary resource local tribes. However, the defense mechanisms underlying ecological adaptations of R. decorum remain to be elucidated. In this study, we conducted comparative transcriptomic analyses various organs (corolla, androecium/gynoecium and leaves) collected from two distinct regions. Approximately 186.98 Gb clean data were generated three across these Through de novo assembly, total 92,025 unigenes obtained nearly half them (43,515 unigenes) successfully annotated. Enrichment analysis differentially expressed genes (DEGs) within groups different (HQI/LFI, HQO/LFO HQL/LFL, respectively) revealed that distribution Heqing region exhibited an increased requirement plant immunity, including resistance diseases, insects, herbivores organs. Conversely, Lijiang showed greater reliance on environmental factors, such cold tolerance, aromatic compounds production, attraction pollinating insects. Notably, validation 21 pivotal identified significantly regulated enrichment pathways functional consistency KEGG among The disparities observed transcriptome regions provide valuable insight into understanding adaptive mechanism.

Language: Английский

Citations

0
Exogenous application of luteolin enhances wheat resistance to Puccinia striiformis f. sp. tritici DOI

Yixi Kong,

Huiyutang Wang,

Liang Qiao

et al.

Plant Physiology and Biochemistry, Journal Year: 2025, Volume and Issue: 222, P. 109674 - 109674

Published: Feb. 21, 2025

Language: Английский

Citations

0
Isolation and Identification of Colletotrichum nymphaeae as a Causal Agent of Leaf Spot on Rhododendron hybridum Ker Gawl and Its Effects on the Ultrastructure of Host Plants DOI Creative Commons
Yajiao Sun, Yao Tian, Jian Liu

et al.

Journal of Fungi, Journal Year: 2025, Volume and Issue: 11(5), P. 392 - 392

Published: May 19, 2025

Rhododendron hybridum Ker Gawl, a widely cultivated horticultural species in China, is highly valued for its ornamental and medicinal properties. However, with the expansion of cultivation, leaf spot disease has become more prevalent, significantly affecting value R. Gawl. In this study, Gawl from Kunming area was selected as experimental material. The tissue isolation method employed study to isolate pathogenic strains. biological characteristics pathogens were determined using mycelial growth rate method. pathogens’ influence on host plant’s ultrastructure investigated transmission electron microscopy (TEM). Colletotrichum nymphaeae identified pathogen implicated development across three regions City through integration morphological traits phylogenetic analyses multiple genes (ITS, ACT, GAPDH, HIS3, CHS1, TUB2). Its most effective at temperature 25 °C. pH light have relatively minor effects mycelium. preferred carbon nitrogen sources mannitol yeast extract, respectively. Additionally, TEM observations revealed significant damage cell structure leaves infected by pathogen. walls dissolved, number chloroplasts decreased markedly, starch granules within largely absent, osmiophilic increased. This first report caused C. nymphaeae. results provide valuable insights future research prevention control disease.

Language: Английский

Citations

0