Genome-Wide Identification of the SlSET Gene Family and the Function of SlSET6 Under Salt Stress DOI Open Access

Xueying Yang,

Hao Yan,

Chengyu Zhu

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(24), P. 13461 - 13461

Published: Dec. 16, 2024

A comprehensive genome-wide identification of SET-domain-containing genes in Solanum lycopersicum (tomato) has revealed 46 members. Phylogenetic analysis showed that these SET genes, along with those from Arabidopsis thaliana and Oryza sativa, are divided into five subfamilies, Subfamilies II V being the largest. Motif domain analyses identified 15 conserved motifs presence pre-SET post-SET domains several suggesting functional diversification. Gene structure further demonstrated variation exon–intron organization, likely contributing to differential gene regulation. Promoter cis-acting elements related light responsiveness, plant growth, hormones, stress, implicating various biological processes. RNA-seq qRT-PCR data distinct expression patterns SlSET under salt showing significant upregulation, indicating their potential role stress tolerance. In particular, SlSET6 silencing using VIGS reduced tomato’s tolerance leading higher lipid peroxidation, antioxidant enzyme activity, decreased proline content, confirming its critical response. These findings provide valuable insights diversity, evolutionary history, stress-related roles tomato, applications for crop improvement strategies.

Language: Английский

GhSERAT1 enhanced the resistance of cotton to cadmium by maintaining the microscopic integrity of chloroplasts in cotton DOI Creative Commons
Yuan Meng,

Yuping Sun,

Ning Wang

et al.

Journal of Hazardous Materials Advances, Journal Year: 2025, Volume and Issue: unknown, P. 100606 - 100606

Published: Jan. 1, 2025

Language: Английский

Citations

0
Identification and Expression Analysis of the Soybean Serine Acetyltransferase (SAT) Gene Family Under Salt Stress DOI Open Access

Caiyun Fan,

Hui Zou, Miao Zhang

et al.

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(5), P. 1882 - 1882

Published: Feb. 22, 2025

Serine acetyltransferase (SAT) is a critical enzyme in the sulfur-assimilation pathway of cysteine, playing an essential role numerous physiological functions plants, particularly their response to environmental stresses. However, structural characteristics soybean SAT gene family remain poorly understood. Members were identified using Hidden Markov Model approach. Bioinformatics tools, such as ExPASy, PlantCARE, MEME, and TBtools-II, employed examine physicochemical properties, cis-regulatory elements, conserved motifs, structures, chromosomal positions GmSAT genes. RT-qPCR was conducted evaluate expression profiles genes under NaCl-induced stress, identifying likely involved salt-stress response. A total ten genome grouped into three subfamilies. Genes within each subfamily shared notable similarities motifs. Analysis elements revealed that promoters these contain several linked plant growth stress-related responses. Expression patterns varied across different tissues, with GmSAT10 showing higher roots, while GmSAT1 GmSAT2 had lower same tissue. Following NaCl treatment, levels seven significantly increased indicating potential involvement plant's adaptation salt stress. appear play crucial roles soybean's offering insights could aid development salt-tolerant varieties.

Language: Английский

Citations

0
Identification of serine acetyltransferase (SAT) gene family in peach (Prunus persica) and study on the function of PpSAT1 gene regulating adventitious root formation DOI Creative Commons
Lili Hao, Fan Zhang, Xuebing Zhang

et al.

Chemical and Biological Technologies in Agriculture, Journal Year: 2025, Volume and Issue: 12(1)

Published: March 31, 2025

Abstract Background Serine acetyltransferase (SAT), an enzyme that catalyzes the formation of O-acetyl-serine (OAS), is integral to sulfur assimilation, cysteine (Cys) synthesis, and adventitious root development. However, it remains unclear how SAT gene in Prunus persica regulates (AR) formation. Methods Based on transcriptome data family identification, physicochemical properties, evolutionary relationships, cis -acting elements genes were analyzed. Subsequently, PpSAT1 was transformed into domestica Arabidopsis thaliana by agrobacterium-mediated method obtain transgenic material, its role AR characterized a series rooting index activity experiments. Results In this study, based data, metabolism pathway significantly enriched during P. growth. After combining FPKM value with real-time fluorescence quantitative qRT-PCR, found SAT1/4 showed high expression level, which may be key peach advection this, members identified from , further shown exhibits notable response Therefore, (Prupe.4G239400.1) cloned performed genetic transformation related as well A. . The displayed more robust growth, developed system compared wild-type counterparts. addition, peroxidase (POD) superoxide dismutase (SOD) activities also substantially elevated. Conclusions summary, these findings suggest can facilitate Graphical

Language: Английский

Citations

0
Genome-Wide Identification of the SlSET Gene Family and the Function of SlSET6 Under Salt Stress DOI Open Access

Xueying Yang,

Hao Yan,

Chengyu Zhu

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(24), P. 13461 - 13461

Published: Dec. 16, 2024

A comprehensive genome-wide identification of SET-domain-containing genes in Solanum lycopersicum (tomato) has revealed 46 members. Phylogenetic analysis showed that these SET genes, along with those from Arabidopsis thaliana and Oryza sativa, are divided into five subfamilies, Subfamilies II V being the largest. Motif domain analyses identified 15 conserved motifs presence pre-SET post-SET domains several suggesting functional diversification. Gene structure further demonstrated variation exon–intron organization, likely contributing to differential gene regulation. Promoter cis-acting elements related light responsiveness, plant growth, hormones, stress, implicating various biological processes. RNA-seq qRT-PCR data distinct expression patterns SlSET under salt showing significant upregulation, indicating their potential role stress tolerance. In particular, SlSET6 silencing using VIGS reduced tomato’s tolerance leading higher lipid peroxidation, antioxidant enzyme activity, decreased proline content, confirming its critical response. These findings provide valuable insights diversity, evolutionary history, stress-related roles tomato, applications for crop improvement strategies.

Language: Английский

Citations

0