The Contribution of Human Antimicrobial Peptides to Fungi

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(6), P. 2494 - 2494

Published: March 11, 2025

Various species of fungi can be detected in the environment and within human body, many which may become pathogenic under specific conditions, leading to various forms fungal infections. Antimicrobial peptides (AMPs) are evolutionarily ancient components immune response that quickly induced infections with pathogens almost all tissues. There is a wide range AMP classes humans, exhibit broad-spectrum antimicrobial function. This review provides comprehensive overview mechanisms action AMPs, their distribution antifungal activity against both common rare clinical pathogens. It also discusses current research status promising novel strategies, highlighting challenges must overcome development these therapies. The hope peptides, as class agents, will soon progress through large-scale trials implemented practice, offering new treatment options for patients suffering from

Language: Английский

Contact-free characterization of nuclear mechanics using correlative Brillouin-Raman Micro-Spectroscopy in living cells

Acta Biomaterialia, Journal Year: 2025, Volume and Issue: unknown

Published: April 1, 2025

Nuclear mechanics is a key parameter in regulating cell physiology, affecting chromatin accessibility and transcriptional regulation. The most established method to characterize the of biological materials at sub-micrometer scale based on atomic force microscopy (AFM). However, its contact-based nature limits direct access nucleus. While some indirect methods have been proposed measure nuclear living cells, readout influenced by overlaying cytoskeleton. For this reason, mechanical measurements isolated nuclei are common strategy overcome issue. impact invasive preparation procedure measured properties still unclear. To address issue, we studied skin fibroblasts probing region extracted using AFM correlative Brillouin-Raman Micro-Spectroscopy (BRMS). latter technique non-invasive image systems 3D, obtaining information chemical sample specific points interest. Using approach, demonstrated that significantly softer than intact ones. Moreover, ability BRMS highlight features within cells were masked convolution with cytosol conventional measurements. Overall, study shows importance evaluating native environment where cellular homeostasis preserved. We, therefore, suggest offers much deeper insight into compared AFM, it should be adopted as reference tool mechanobiology. STATEMENT OF SIGNIFICANCE: nucleus, largest eukaryotic organelle, crucial for function genetic material storage. Its properties, often altered disease, influence processes like accessibility. Although (AFM) standard studying mechanics, isolating stiffness challenging due interference from cytoskeleton plasma membrane. We demonstrate (BRMS) enables non-contact, high-resolution measurement capturing sub-micron details. compare results obtained same samples AFM. enhances our understanding physiological conditions, offering valuable insights researchers field mechanobiology, biotechnology, medicine, bioengineering.

Language: Английский

Overview of Biomaterials Classification, Properties, and Biomedical Applications

IGI Global eBooks, Journal Year: 2025, Volume and Issue: unknown, P. 51 - 90

Published: April 25, 2025

Biomaterials have attracted growing interest in the biomedical field thanks to their unique ability interact with biological systems and make a significant contribution advances medicine, tissue engineering sustainable technologies. Their importance lies dual potential: improving patients' quality of life while reducing impact on environment. This chapter first presents detailed classification biomaterials, distinguishing between natural, synthetic hybrid categories. Next, key properties biomaterials are explored, showing role biocompatibility, durability clinical performance. These fundamental characteristics directly influence choice for variety applications. In addition, this highlights diversity current biomaterial applications, discussing persistent challenges limitations encountered design use. Finally, future prospects discussed, including emergence personalized which could revolutionize

Language: Английский