Study on the Chemical Composition and Multidrug Resistance Reversal Activity of Euphorbia uralensis (Euphorbiaceae) DOI Open Access

Yina Ding,

Yuhao Liu, Qiang Dang

et al.

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(1), P. 412 - 412

Published: Jan. 6, 2025

Euphorbia uralensis belongs to the family Euphorbiaceae and is widely distributed in northern Xinjiang, making it a characteristic plant of region China. The chemical composition biological activity have not yet been reported, although certain compounds isolated from plants China, demonstrated exceptional multidrug resistance (MDR) reversal. This study aims investigate components present with potential reverse MDR. aerial parts were extracted using organic solvents varying polarities, resulting dichloromethane (Fr-E) petroleum ether (Fr-S) fractions, which exhibited greater MDR reversal than other fractions. constituents Fr-S fraction analyzed GC-MS. Fr-E purified column chromatography. most effective screened out, mechanism was investigated molecular docking, dynamics simulations, Western blotting, rhodamine 123 staining. GC-MS analysis showed that rich triterpenes, fatty acids, phenols, long-chain alkanes, all identified for first time uralensis. Among these, palmitic acid at content level 15.86%. notably unveils discovery new compound 16 previously recorded this plant, main types as steroids, sesquiterpenes, flavonoids. tested cytotoxicity activity. Euphouralosides A, pubinernoid naringenin, punigratine good against MCF-7 MCF-7/ADR cell lines. Punigratine active compound. Moreover, could stably bind ABCB1 protein. blot revealed did affect expression protein cells (p > 0.05). However, following treatment (0.16 μM), there significant increase intracellular accumulation Rh123 < These findings suggest can inhibit efflux protein, thereby overcoming tumors. provides foundation further research on medicinal

Language: Английский

Study on the Chemical Composition and Multidrug Resistance Reversal Activity of Euphorbia uralensis (Euphorbiaceae) DOI Open Access

Yina Ding,

Yuhao Liu, Qiang Dang

et al.

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(1), P. 412 - 412

Published: Jan. 6, 2025

Euphorbia uralensis belongs to the family Euphorbiaceae and is widely distributed in northern Xinjiang, making it a characteristic plant of region China. The chemical composition biological activity have not yet been reported, although certain compounds isolated from plants China, demonstrated exceptional multidrug resistance (MDR) reversal. This study aims investigate components present with potential reverse MDR. aerial parts were extracted using organic solvents varying polarities, resulting dichloromethane (Fr-E) petroleum ether (Fr-S) fractions, which exhibited greater MDR reversal than other fractions. constituents Fr-S fraction analyzed GC-MS. Fr-E purified column chromatography. most effective screened out, mechanism was investigated molecular docking, dynamics simulations, Western blotting, rhodamine 123 staining. GC-MS analysis showed that rich triterpenes, fatty acids, phenols, long-chain alkanes, all identified for first time uralensis. Among these, palmitic acid at content level 15.86%. notably unveils discovery new compound 16 previously recorded this plant, main types as steroids, sesquiterpenes, flavonoids. tested cytotoxicity activity. Euphouralosides A, pubinernoid naringenin, punigratine good against MCF-7 MCF-7/ADR cell lines. Punigratine active compound. Moreover, could stably bind ABCB1 protein. blot revealed did affect expression protein cells (p > 0.05). However, following treatment (0.16 μM), there significant increase intracellular accumulation Rh123 < These findings suggest can inhibit efflux protein, thereby overcoming tumors. provides foundation further research on medicinal

Language: Английский

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