Alternative Splicing Events and ABA Hormone Regulation in Drought Response of Hippophae gyantsensis L.

Genes, Journal Year: 2025, Volume and Issue: 16(3), P. 350 - 350

Published: March 18, 2025

(1) Background: Hippophae gyantsensis, a drought-tolerant plant native to the Tibetan Plateau, plays crucial ecological and economic role. While its drought tolerance mechanisms have been extensively studied, role of alternative splicing (AS) in resistance remains insufficiently explored. This study aims elucidate how AS events regulate gene expression enhance H. gyantsensis under water-deficit conditions. (2) Methods: plants were subjected progressive stress followed by rehydration. Physiological responses, transcriptomic data, hormonal profiles analyzed investigate plant’s adaptive stress, with particular focus on abscisic acid (ABA) signaling-related genes. (3) Results: The results showed that maintained high leaf water content even severe emphasizing strong resistance. A analysis revealed 11,962 differentially expressed genes, primarily enriched hormone signaling metabolic pathways. Notably, accumulation ABA was closely associated ABA-related such as ZEPs, ABCG, PP2C. These genes produced multiple splice variants, indicating their modulating pathway enhancing tolerance. (4) Conclusions: highlights pivotal gyantsensis. It provides new insights into contributes adaptation bridging knowledge gap importance responses.

Language: Английский

Exploring Alternative Splicing in Response to Salinity: A Tissue-Level Comparative Analysis Using Arabidopsis thaliana Public Transcriptomic Data

Plants, Journal Year: 2025, Volume and Issue: 14(7), P. 1064 - 1064

Published: March 30, 2025

Increased soil salinity is a major threat to global agriculture and food security, caused mainly by anthropogenic activities changing climatic cycles. Plants responses involve multiple regulatory layers, from transcriptome reprogramming proteomic metabolomic changes. Alternative splicing (AS) plays role in coordinating the response salinity, yet its extent, tissue, condition specificity, remain poorly understood aspects. In this study, we used 52 publicly available RNA-seq datasets of treatment identify differential alternative (DAS) events genes participating stimulus. Our findings reveal that either independently or coordinately, AS can regulate up 20% detected Arabidopsis, with intensity being most determining factor. Moreover, show regulation was highly tissue-specific, roots displaying strong AS-mediated stress responses. Furthermore, cross-stress comparisons showed have core set AS-regulated associated development, functionally distinct sets when comparing salt other stresses, while also conserving relevant condition-specific response. We demonstrate need integrate analysis better understand plant adaptation mechanisms highlight key responses, revealing shared between salt, heat, drought

Language: Английский

Alternative splicing analysis of stress tolerance to Al and flg22 in Vitis quinquangularis

Planta, Journal Year: 2025, Volume and Issue: 261(6)

Published: May 14, 2025

Language: Английский