Differential Expression of ARG1 and MRC2 in Retinal Müller Glial Cells During Autoimmune Uveitis DOI Creative Commons
Alan B. Fleischer, Barbara Amann, Christine von Toerne

et al.

Biomolecules, Journal Year: 2025, Volume and Issue: 15(2), P. 288 - 288

Published: Feb. 14, 2025

Retinal Müller glial cells (RMG) play a crucial role in retinal neuroinflammation, including autoimmune uveitis. Increasing evidence supports their function as active modulators of immune responses and potential atypical antigen-presenting (APCs). To further investigate this hypothesis, we conducted differential proteome analysis primary equine RMG from healthy controls horses with recurrent uveitis (ERU), spontaneous model This identified 310 proteins abundance. Among these, the Major Histocompatibility Complex (MHC) class II enzyme Arginase 1 (ARG1) were significantly enriched uveitis-affected horses, whereas Mannose Receptor C-type 2 (MRC2) its interactor Thrombospondin (THBS1) more abundant RMG. The detection MHC RMG, consistent previous studies, validates robustness our approach. Furthermore, identification ARG1 MRC2, together THBS1, provides new insights into immunomodulatory properties Immunohistochemical analyses confirmed proteomic findings revealed spatial distribution MRC2. MRC2 are thus markers for neuroinflammatory or physiological milieu highlight differences particularly antigen presentation.

Language: Английский

Differential Expression of ARG1 and MRC2 in Retinal Müller Glial Cells During Autoimmune Uveitis DOI Creative Commons
Alan B. Fleischer, Barbara Amann, Christine von Toerne

et al.

Biomolecules, Journal Year: 2025, Volume and Issue: 15(2), P. 288 - 288

Published: Feb. 14, 2025

Retinal Müller glial cells (RMG) play a crucial role in retinal neuroinflammation, including autoimmune uveitis. Increasing evidence supports their function as active modulators of immune responses and potential atypical antigen-presenting (APCs). To further investigate this hypothesis, we conducted differential proteome analysis primary equine RMG from healthy controls horses with recurrent uveitis (ERU), spontaneous model This identified 310 proteins abundance. Among these, the Major Histocompatibility Complex (MHC) class II enzyme Arginase 1 (ARG1) were significantly enriched uveitis-affected horses, whereas Mannose Receptor C-type 2 (MRC2) its interactor Thrombospondin (THBS1) more abundant RMG. The detection MHC RMG, consistent previous studies, validates robustness our approach. Furthermore, identification ARG1 MRC2, together THBS1, provides new insights into immunomodulatory properties Immunohistochemical analyses confirmed proteomic findings revealed spatial distribution MRC2. MRC2 are thus markers for neuroinflammatory or physiological milieu highlight differences particularly antigen presentation.

Language: Английский

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