CircATP5C1 promotes triple-negative breast cancer progression by binding IGF2BP2 to modulate CSF-1 secretion DOI Creative Commons
Hongbo Liu, Haoqi Wang, Wei Gao

et al.

Cancer Biology & Therapy, Journal Year: 2025, Volume and Issue: 26(1)

Published: April 2, 2025

Triple-negative breast cancer (TNBC) is a common malignant disease among females and severely threatens the health of women worldwide. Nowadays, circular RNAs (circRNAs) aroused our interest for their functions in human cancers, including TNBC. However, mechanism most circRNAs progression TNBC remains unclear. We found novel circRNA named circATP5C1, whose function uncovered. Tissue microarray was used to analyze association between expression circATP5C1 prognoses patients. Gain-and loss-of-function experiments were performed validate biological different cell lines. RNA-seq analyses conducted find out target genes regulated by circATP5C1. RNA pull-down assay mass spectrometry select proteins associated with FISH-immunofluorescence immunoprecipitation (RIP) complemented interaction its binding protein. CircATP5C1 identified have predictive prognosis advanced cells. Mechanistically, Colony stimulating factor 1 (CSF-1) vital downstream gene The alteration CSF-1 level validated due insulin-like growth 2 mRNA protein (IGF2BP2). Rescue demonstrated that accelerates partly via IGF2BP2 increase secretion CSF-1. This study uncovers circATP5C1/IGF2BP2/CSF-1 pathway regulating

Language: Английский

CircATP5C1 promotes triple-negative breast cancer progression by binding IGF2BP2 to modulate CSF-1 secretion DOI Creative Commons
Hongbo Liu, Haoqi Wang, Wei Gao

et al.

Cancer Biology & Therapy, Journal Year: 2025, Volume and Issue: 26(1)

Published: April 2, 2025

Triple-negative breast cancer (TNBC) is a common malignant disease among females and severely threatens the health of women worldwide. Nowadays, circular RNAs (circRNAs) aroused our interest for their functions in human cancers, including TNBC. However, mechanism most circRNAs progression TNBC remains unclear. We found novel circRNA named circATP5C1, whose function uncovered. Tissue microarray was used to analyze association between expression circATP5C1 prognoses patients. Gain-and loss-of-function experiments were performed validate biological different cell lines. RNA-seq analyses conducted find out target genes regulated by circATP5C1. RNA pull-down assay mass spectrometry select proteins associated with FISH-immunofluorescence immunoprecipitation (RIP) complemented interaction its binding protein. CircATP5C1 identified have predictive prognosis advanced cells. Mechanistically, Colony stimulating factor 1 (CSF-1) vital downstream gene The alteration CSF-1 level validated due insulin-like growth 2 mRNA protein (IGF2BP2). Rescue demonstrated that accelerates partly via IGF2BP2 increase secretion CSF-1. This study uncovers circATP5C1/IGF2BP2/CSF-1 pathway regulating

Language: Английский

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