Transcriptome Analysis Revealed That Cell Wall Regulatory Pathways Are Involved in the Tolerance of Pleurotus ostreatus Mycelia to Different Heat Stresses DOI Creative Commons

Ludan Hou,

Jingyi Wang,

Tonglou Li

et al.

Journal of Fungi, Journal Year: 2025, Volume and Issue: 11(4), P. 266 - 266

Published: March 30, 2025

Pleurotus ostreatus is the third largest cultivated species in China’s edible mushroom industry; however, its agricultural cultivation method easily affected by high-temperature environments. To understand response mechanism of mycelia to heat stress, P. ostreatus, which had been grown at 28 °C for 4 days, were subjected stress 32 and 36 2 followed RNA-seq analysis. These results indicate that, under mycelial growth was significantly inhibited, cell membrane disrupted, walls became thicker, chitinase β-1,3-glucanase activities decreased. Transcriptome analysis revealed 2118 differentially expressed genes (DEGs) 458 DEGs identified stress. A total 328 upregulated or downregulated °C. The functional enrichment these significant related hydrogen peroxide metabolism, oxidoreductase activity, ATP hydrolysis, wall structure composition. There a 80 specific °C, they enriched catalase wall, aminoglycan catabolic process, activity. However, 817 integral components membrane, identification wall-related that hydrophobic proteins, Cerato plateau laccases, glycoside hydrolases may respond qRT-PCR are consistent with data. This study several potential candidate thermal response, laying foundation ostreatus.

Language: Английский

Transcriptome Analysis Revealed That Cell Wall Regulatory Pathways Are Involved in the Tolerance of Pleurotus ostreatus Mycelia to Different Heat Stresses DOI Creative Commons

Ludan Hou,

Jingyi Wang,

Tonglou Li

et al.

Journal of Fungi, Journal Year: 2025, Volume and Issue: 11(4), P. 266 - 266

Published: March 30, 2025

Pleurotus ostreatus is the third largest cultivated species in China’s edible mushroom industry; however, its agricultural cultivation method easily affected by high-temperature environments. To understand response mechanism of mycelia to heat stress, P. ostreatus, which had been grown at 28 °C for 4 days, were subjected stress 32 and 36 2 followed RNA-seq analysis. These results indicate that, under mycelial growth was significantly inhibited, cell membrane disrupted, walls became thicker, chitinase β-1,3-glucanase activities decreased. Transcriptome analysis revealed 2118 differentially expressed genes (DEGs) 458 DEGs identified stress. A total 328 upregulated or downregulated °C. The functional enrichment these significant related hydrogen peroxide metabolism, oxidoreductase activity, ATP hydrolysis, wall structure composition. There a 80 specific °C, they enriched catalase wall, aminoglycan catabolic process, activity. However, 817 integral components membrane, identification wall-related that hydrophobic proteins, Cerato plateau laccases, glycoside hydrolases may respond qRT-PCR are consistent with data. This study several potential candidate thermal response, laying foundation ostreatus.

Language: Английский

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