Fourier Transform Infrared Spectroscopy Analysis as a Tool to Address Aβ Impact on Extracellular Vesicles DOI Creative Commons
Margarida Vaz, Tânia Soares Martins, Kevin Leandro

et al.

Molecules, Journal Year: 2025, Volume and Issue: 30(2), P. 258 - 258

Published: Jan. 10, 2025

Alzheimer's disease is a challenge in modern healthcare due to its complex etiology and increasing prevalence. Despite advances, further understanding of pathophysiology needed, particularly the role Aβ neurotoxic peptide. Fourier transform infrared spectroscopy (FTIR) has shown potential as screening tool for several pathologies, including disease. Nonetheless, limited research explored direct effects on neurons extracellular vesicles metabolic profiles. Hence, this study aims investigate impact spectroscopic profiles neuronal-like cells (N2a) N2a-derived vesicles, employing FTIR focusing 1280-900 cm-1 region. A comprehensive analysis spectral data was carried out, multivariate partial least squares (PLS) peak intensities analysis. PLS revealed moderate strong correlations within region both N2a vesicles. The intensity additional peaks with significant differences EVs' spectra relative N2a, following treatment. Specifically, seems cause alterations protein phosphorylation nucleic acids content These findings support that Aβ's pathology may be mediated by highlight FTIR's advancing clinical applications.

Language: Английский

Fourier Transform Infrared Spectroscopy Analysis as a Tool to Address Aβ Impact on Extracellular Vesicles DOI Creative Commons
Margarida Vaz, Tânia Soares Martins, Kevin Leandro

et al.

Molecules, Journal Year: 2025, Volume and Issue: 30(2), P. 258 - 258

Published: Jan. 10, 2025

Alzheimer's disease is a challenge in modern healthcare due to its complex etiology and increasing prevalence. Despite advances, further understanding of pathophysiology needed, particularly the role Aβ neurotoxic peptide. Fourier transform infrared spectroscopy (FTIR) has shown potential as screening tool for several pathologies, including disease. Nonetheless, limited research explored direct effects on neurons extracellular vesicles metabolic profiles. Hence, this study aims investigate impact spectroscopic profiles neuronal-like cells (N2a) N2a-derived vesicles, employing FTIR focusing 1280-900 cm-1 region. A comprehensive analysis spectral data was carried out, multivariate partial least squares (PLS) peak intensities analysis. PLS revealed moderate strong correlations within region both N2a vesicles. The intensity additional peaks with significant differences EVs' spectra relative N2a, following treatment. Specifically, seems cause alterations protein phosphorylation nucleic acids content These findings support that Aβ's pathology may be mediated by highlight FTIR's advancing clinical applications.

Language: Английский

Citations

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