CRISPR-Cas Systems: A Functional Perspective and Innovations DOI Open Access
Carla Navarro, María P. Díaz, Pablo Durán

et al.

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(8), P. 3645 - 3645

Published: April 12, 2025

Adaptation is a fundamental tenet of evolutionary biology and essential for the survival all organisms, including prokaryotes. The evolution clustered regularity exemplifies this principle interspaced short palindromic repeats (CRISPR) associated proteins (Cas), an adaptive immune system that confers resistance to viral infections. By integrating segments genomes into their own, bacteria archaea develop molecular memory enables them mount rapid targeted response upon subsequent challenges. fortuitous discovery mechanism prompted many studies introduced researchers novel tools could potentially be developed from CRISPR-Cas become clinically relevant as biotechnology rapidly advances in area. Thus, deeper understanding underpinnings its possible therapeutic applications required. This review analyses action systems detail summarises developing biotechnological based on CRISPR, opening field further research.

Language: Английский

Systematic trans‐Activity Comparison of Several Reported Cas12f Nucleases† DOI Open Access
Yannan Wang, Na Tang, Quanjiang Ji

et al.

Chinese Journal of Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: March 18, 2025

Comprehensive Summary Cas12f possesses both cis ‐ and trans ‐cleavage activities, with the former being extensively studied for its application in genome editing, while latter remains less explored, particularly diagnostic purposes, is mostly focused on Un1Cas12f1. In this study, we conducted a comprehensive comparison of activities four characterized proteins, demonstrating that all exhibit ‐DNase activity triggered by double‐stranded DNA (dsDNA), single‐stranded (ssDNA), RNA (ssRNA). Additionally, identified distinct base preferences substrates among these proteins. Our further investigation into revealed intricate relationship between under various conditions. study provides multifaceted characterization features nucleases, offering new avenues deeper comprehension mechanisms underlying Cas12f's functionality.

Language: Английский

Citations

0
CRISPR-Cas Systems: A Functional Perspective and Innovations DOI Open Access
Carla Navarro, María P. Díaz, Pablo Durán

et al.

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(8), P. 3645 - 3645

Published: April 12, 2025

Adaptation is a fundamental tenet of evolutionary biology and essential for the survival all organisms, including prokaryotes. The evolution clustered regularity exemplifies this principle interspaced short palindromic repeats (CRISPR) associated proteins (Cas), an adaptive immune system that confers resistance to viral infections. By integrating segments genomes into their own, bacteria archaea develop molecular memory enables them mount rapid targeted response upon subsequent challenges. fortuitous discovery mechanism prompted many studies introduced researchers novel tools could potentially be developed from CRISPR-Cas become clinically relevant as biotechnology rapidly advances in area. Thus, deeper understanding underpinnings its possible therapeutic applications required. This review analyses action systems detail summarises developing biotechnological based on CRISPR, opening field further research.

Language: Английский

Citations

0