Advances in Sampling and Analytical Techniques for Single‐Cell Metabolomics: Exploring Cellular Heterogeneity DOI

Xinxin Shen,

Fangyuan Zhang, Chunping Tang

et al.

Rapid Communications in Mass Spectrometry, Journal Year: 2025, Volume and Issue: 39(13)

Published: April 13, 2025

ABSTRACT Single‐cell metabolomics is an emerging and powerful technology that uncovers intercellular heterogeneity reveals microenvironmental dynamics in both physiological pathological conditions. This enables detailed observations of cellular interactions, providing valuable insights into processes such as aging, immune responses, disease development. Despite significant advances, the need for discussions on sampling analytical methods single‐cell continues to grow, with increasing focus selecting most suitable techniques diverse research objectives. review addresses these challenges by exploring key strategies used metabolomics. We three main approaches: capture isolation specific cell types, precise aspiration individual cells, situ mass spectrometry imaging. These are critically assessed highlight achieving accurate metabolite detection at level across applications.

Language: Английский

Targeting fatty acid oxidation: A potential strategy for treating gastrointestinal tumors DOI Open Access

Yaxi Zheng,

Zhengchen Jiang,

Li Yuan

et al.

International Journal of Cancer, Journal Year: 2025, Volume and Issue: unknown

Published: March 6, 2025

Gastrointestinal cancers including esophageal squamous cell carcinoma (ESCC), gastric cancer (GC), and colorectal (CRC) are common highly lethal types of worldwide. Metabolic reprogramming plays a critical role in progression involves metabolic processes such as glucose lipid metabolism. Fatty acid oxidation (FAO) has profound impact on cancer, with many genes cytokines influencing initiation, development, metastasis, resistance by regulating FAO. Additionally, FAO further promotes affecting the tumor microenvironment (TME). The gastrointestinal garnered increasing attention, related anticancer drugs currently being developed.

Language: Английский

Citations

1
Identification of specific genes related to efficient Monensin biosynthesis in Streptomyces cinnamonensis with genomic analysis DOI
Shanfei Zhang, Zhenhua Wang, Qingming Hou

et al.

Systems Microbiology and Biomanufacturing, Journal Year: 2025, Volume and Issue: unknown

Published: March 25, 2025

Language: Английский

Citations

0
Dietary bile acids reduced ferroptosis, improved the flesh quality and intestinal health of Pacific white shrimp (Litopenaeus vannamei) fed the high plant protein-based diets DOI

Kangyuan Qu,

Junwei Zeng, Junming Deng

et al.

Aquaculture, Journal Year: 2025, Volume and Issue: unknown, P. 742562 - 742562

Published: April 1, 2025

Language: Английский

Citations

0
Advances in Sampling and Analytical Techniques for Single‐Cell Metabolomics: Exploring Cellular Heterogeneity DOI

Xinxin Shen,

Fangyuan Zhang, Chunping Tang

et al.

Rapid Communications in Mass Spectrometry, Journal Year: 2025, Volume and Issue: 39(13)

Published: April 13, 2025

ABSTRACT Single‐cell metabolomics is an emerging and powerful technology that uncovers intercellular heterogeneity reveals microenvironmental dynamics in both physiological pathological conditions. This enables detailed observations of cellular interactions, providing valuable insights into processes such as aging, immune responses, disease development. Despite significant advances, the need for discussions on sampling analytical methods single‐cell continues to grow, with increasing focus selecting most suitable techniques diverse research objectives. review addresses these challenges by exploring key strategies used metabolomics. We three main approaches: capture isolation specific cell types, precise aspiration individual cells, situ mass spectrometry imaging. These are critically assessed highlight achieving accurate metabolite detection at level across applications.

Language: Английский

Citations

0