The Identification of a Glucuronyltransferase-Related Gene, GlcAT-S, with Putative Mucus Protection and Anti-Inflammatory Effects from Gut-Damaged Drosophila by Dextran Sulfate Sodium (DSS) DOI Creative Commons
Seung Hun Lee,

Dooseon Hwang,

Jang‐Won Lee

et al.

Biology, Journal Year: 2025, Volume and Issue: 14(5), P. 513 - 513

Published: May 7, 2025

The intestinal epithelium, which is protected by mucosal surfaces composed of mucins and other glycoproteins, functions as a selective barrier that absorbs nutrients while preventing the translocation harmful substances. To understand mechanisms between disruption tissue inflammation, we orally administrated mucus-disrupting agent, dextran sodium sulfate, to Drosophila melanogaster screened 63 differentially expressed genes (DEGs). Through database search using bioinformatics tools (CHEA3 WebGestalt), identified ELK1 potential key transcription factor for selected DEGs, among ELK1-related genes, B3GAT3, FIBP, TENT2 (GlcAT-S, Fibp, Wisp in Drosophila), were relevant respond mucus disruption. We confirmed enterocyte (EC)-specific GlcAT-S knockdown RNAi significantly reduced gut length increased stem cell proliferation Drosophila. Additionally, EC-specific GlcAT-S-knockdown flies, it was observed mucus-production-related Muc68D Mur29B, specifically reduced, whereas inflammatory cytokines egr upd3 overexpressed. This study provides evidence involved regulation inflammation plays protective role against Our findings suggest may be therapeutic target treatment diseases such IBD.

Language: Английский

Nrf2 Signaling Pathway Studies in Drosophila melanogaster : Parallel Roles in Human Health and Insect Environmental Responses DOI
Jing Li, Shixiang Sun, Ying Li

et al.

Xenobiotica, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 30

Published: Feb. 11, 2025

The Nrf2 signaling pathway is crucial for cellular defense against oxidative stress and xenobiotic toxicity, highlighting its importance in both human health environmental responses.

Language: Английский

Citations

0
The Identification of a Glucuronyltransferase-Related Gene, GlcAT-S, with Putative Mucus Protection and Anti-Inflammatory Effects from Gut-Damaged Drosophila by Dextran Sulfate Sodium (DSS) DOI Creative Commons
Seung Hun Lee,

Dooseon Hwang,

Jang‐Won Lee

et al.

Biology, Journal Year: 2025, Volume and Issue: 14(5), P. 513 - 513

Published: May 7, 2025

The intestinal epithelium, which is protected by mucosal surfaces composed of mucins and other glycoproteins, functions as a selective barrier that absorbs nutrients while preventing the translocation harmful substances. To understand mechanisms between disruption tissue inflammation, we orally administrated mucus-disrupting agent, dextran sodium sulfate, to Drosophila melanogaster screened 63 differentially expressed genes (DEGs). Through database search using bioinformatics tools (CHEA3 WebGestalt), identified ELK1 potential key transcription factor for selected DEGs, among ELK1-related genes, B3GAT3, FIBP, TENT2 (GlcAT-S, Fibp, Wisp in Drosophila), were relevant respond mucus disruption. We confirmed enterocyte (EC)-specific GlcAT-S knockdown RNAi significantly reduced gut length increased stem cell proliferation Drosophila. Additionally, EC-specific GlcAT-S-knockdown flies, it was observed mucus-production-related Muc68D Mur29B, specifically reduced, whereas inflammatory cytokines egr upd3 overexpressed. This study provides evidence involved regulation inflammation plays protective role against Our findings suggest may be therapeutic target treatment diseases such IBD.

Language: Английский

Citations

0