Identification of E3 Ubiquitin Ligase Substrates Using Biotin Ligase-Based Proximity Labeling Approaches DOI Creative Commons
Koji Matsuhisa, Shinya Sato, Masayuki Kaneko

et al.

Biomedicines, Journal Year: 2025, Volume and Issue: 13(4), P. 854 - 854

Published: April 2, 2025

Ubiquitylation is a post-translational modification originally identified as the first step in protein degradation by ubiquitin–proteasome system. also known to regulate many cellular processes without degrading ubiquitylated proteins. Substrate proteins are specifically recognized and ubiquitin ligases. It necessary identify substrates for each ligase understand physiological pathological roles of ubiquitylation. Recently, promiscuous mutant biotin derived from Escherichia coli, BioID, its variants have been utilized analyze protein–protein interaction. In this review, we summarize current knowledge regarding molecular mechanisms underlying ubiquitylation, BioID-based approaches interactome studies, application BirA identification substrates.

Language: Английский

Molecular Abnormalities and Carcinogenesis in Barrett’s Esophagus: Implications for Cancer Treatment and Prevention DOI Open Access

Thaís Cabral de Melo Viana,

E. Nakamura, Amanda Park

et al.

Genes, Journal Year: 2025, Volume and Issue: 16(3), P. 270 - 270

Published: Feb. 25, 2025

Background: Barrett’s esophagus (BE) is described by the transformation of normal squamous epithelium into metaplastic columnar epithelium, driven chronic gastroesophageal reflux disease (GERD). BE a recognized premalignant condition and main precursor to esophageal adenocarcinoma (EAC). Understanding molecular mechanisms underlying carcinogenesis crucial for improving prevention, surveillance, treatment strategies. Methods: This narrative review examines abnormalities associated with progression EAC. Results: study highlights inflammatory, genetic, epigenetic, chromosomal alterations, emphasizing key pathways biomarkers. follows multistep process involving dysplasia genetic alterations such as TP53 CDKN2A (p16) mutations, instability, dysregulation like PI3K/AKT/mTOR. Epigenetic including aberrant microRNA expression or DNA methylation, further contribute this progression. These changes are stage-specific, some occurring early in during transition high-grade Innovations chemoprevention, combining proton pump inhibitors aspirin, potential antireflux surgery halt promising. Incorporating biomarkers surveillance strategies advancing precision medicine may enable earlier detection personalized treatments. Conclusions: primary preneoplastic A deeper understanding its can enhance protocols, optimize management inflammation, refine prevention therapeutic strategies, ultimately contributing reduction global burden

Language: Английский

Citations

0
Molecular Mechanisms in the Transformation from Indolent to Aggressive B Cell Malignancies DOI Open Access
Nawar Maher, Samir Mouhssine, Bassam Francis Matti

et al.

Cancers, Journal Year: 2025, Volume and Issue: 17(5), P. 907 - 907

Published: March 6, 2025

Histological transformation (HT) into aggressive lymphoma is a turning point in significant fraction of patients affected by indolent lymphoproliferative neoplasms, namely, chronic lymphocytic leukemia (CLL), follicular (FL), marginal zone lymphomas (MZLs), and lymphoplasmacytic (LPL) [...]

Language: Английский

Citations

0
Prunin: An Emerging Anticancer Flavonoid DOI Open Access
Juie Nahushkumar Rana, Sohail Mumtaz

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(6), P. 2678 - 2678

Published: March 16, 2025

Despite the substantial advances in cancer therapies, developing safe and effective treatment methodologies is critical. Natural (plant-derived compounds), such as flavonoids, might be crucial a methodology without toxicity toward healthy tissues. Prunin flavonoid with potential to used biomedical applications. has yet undergo thorough scientific research, its precise molecular mechanisms of action remain largely unexplored. This review summarizes therapeutic prunin for first time, focusing on underlying an anticancer compound. gained significant attention due antioxidant, anti-inflammatory, effects. aims unlock how functions at level exert effects, primarily modulating key cellular pathways. Furthermore, we have discussed prunin’s adjunctive therapy conventional treatments, highlighting ability strengthen responses while decreasing drug resistance. Moreover, discussion probes into innovative delivery methods, particularly nanoformulations, that address bioavailability, solubility, stability limitations optimize application. By providing comprehensive analysis properties, this stimulate further exploration using agent, thereby progressing development targeted, selective, safe, methods.

Language: Английский

Citations

0
Identification of E3 Ubiquitin Ligase Substrates Using Biotin Ligase-Based Proximity Labeling Approaches DOI Creative Commons
Koji Matsuhisa, Shinya Sato, Masayuki Kaneko

et al.

Biomedicines, Journal Year: 2025, Volume and Issue: 13(4), P. 854 - 854

Published: April 2, 2025

Ubiquitylation is a post-translational modification originally identified as the first step in protein degradation by ubiquitin–proteasome system. also known to regulate many cellular processes without degrading ubiquitylated proteins. Substrate proteins are specifically recognized and ubiquitin ligases. It necessary identify substrates for each ligase understand physiological pathological roles of ubiquitylation. Recently, promiscuous mutant biotin derived from Escherichia coli, BioID, its variants have been utilized analyze protein–protein interaction. In this review, we summarize current knowledge regarding molecular mechanisms underlying ubiquitylation, BioID-based approaches interactome studies, application BirA identification substrates.

Language: Английский

Citations

0