Cyclophilin A and C are the Main Components of Extracellular Vesicles in Response to Hyperglycemia in BV2 Microglial Cells DOI Creative Commons

Noelia Castedo,

Amparo Alfonso, Rebeca Alvariño

et al.

Molecular Neurobiology, Journal Year: 2025, Volume and Issue: unknown

Published: April 8, 2025

Abstract Cyclophilins (Cyps) and CD147 receptor play a crucial role in the inflammatory responses. Chronic inflammation causes tissue damage is common condition of several inflammation-based pathologies as diabetes or Alzheimer´s disease. Under high glucose (HG) conditions, microglia activated releases mediators. In this process Cyps unknown, so study was aimed to investigate profile their release through extracellular vesicles (EVs) under hyperglycemia. An increase reactive oxygen species (ROS) nitric oxide (NO) levels observed when BV2 glia cells were incubated with HG concentration. These effects mitigated by inhibitor cyclosporine A (CsA), suggesting implication activation. these conditions intracellular expression CypA, B, C D, well membrane increased. addition, only CypA CypC detected medium. Then, presence inside EVs explored an alternative secretion route. Interestingly, treatment, four observed. When neurons treated derived from HG-treated cells, viability reduced cytosol pointing EVs-Cyps neurotoxic effect. findings provide novel insights into relationship between neuroinflammation hyperglycemia conditions. The current results strengthen cell communication its potential brain function pathological

Language: Английский

Cyclophilin A and C are the Main Components of Extracellular Vesicles in Response to Hyperglycemia in BV2 Microglial Cells DOI Creative Commons

Noelia Castedo,

Amparo Alfonso, Rebeca Alvariño

et al.

Molecular Neurobiology, Journal Year: 2025, Volume and Issue: unknown

Published: April 8, 2025

Abstract Cyclophilins (Cyps) and CD147 receptor play a crucial role in the inflammatory responses. Chronic inflammation causes tissue damage is common condition of several inflammation-based pathologies as diabetes or Alzheimer´s disease. Under high glucose (HG) conditions, microglia activated releases mediators. In this process Cyps unknown, so study was aimed to investigate profile their release through extracellular vesicles (EVs) under hyperglycemia. An increase reactive oxygen species (ROS) nitric oxide (NO) levels observed when BV2 glia cells were incubated with HG concentration. These effects mitigated by inhibitor cyclosporine A (CsA), suggesting implication activation. these conditions intracellular expression CypA, B, C D, well membrane increased. addition, only CypA CypC detected medium. Then, presence inside EVs explored an alternative secretion route. Interestingly, treatment, four observed. When neurons treated derived from HG-treated cells, viability reduced cytosol pointing EVs-Cyps neurotoxic effect. findings provide novel insights into relationship between neuroinflammation hyperglycemia conditions. The current results strengthen cell communication its potential brain function pathological

Language: Английский

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