MicroRNA-155-5p rs767649 Polymorphism is Associated with Susceptibility to Essential Hypertension in the Chinese Tibetan Population in the Gannan Area
Artery Research,
Journal Year:
2025,
Volume and Issue:
31(1)
Published: Feb. 25, 2025
Language: Английский
IRF5 Mediates Artery Inflammation in Salt-Sensitive Hypertension by Regulating STAT1 and STAT2 Phosphorylation to Increase ESM1 Transcription: Insights from Bioinformatics and Mechanistic Analysis
International Journal of Molecular Sciences,
Journal Year:
2025,
Volume and Issue:
26(8), P. 3722 - 3722
Published: April 15, 2025
Salt-sensitive
hypertension
(SSH)
is
closely
associated
with
arterial
inflammation,
yet
its
molecular
mechanisms
remain
unclear.
In
this
study,
we
utilized
deoxycorticosterone
acetate
(DOCA)-salt-induced
hypertensive
mice,
which
exhibited
elevated
blood
pressure
and
significant
inflammation.
Single-cell
RNA
sequencing
(scRNA-seq)
identified
interferon
regulatory
factor
5
(IRF5)
downstream
targets,
signal
transducer
activator
of
transcription
(STAT),
as
key
regulators
these
inflammatory
changes.
vivo,
IRF5
levels
were
significantly
in
the
DOCA
group,
while
STAT1
STAT2
protein
comparable
to
those
normal
salt
group.
However,
nuclear
phosphorylated
(pSTAT1)
(pSTAT2)
markedly
higher
Furthermore,
scRNA-seq
analysis
showed
increased
expression
endothelial
cells
(ECs)
both
human
mouse
aorta
samples.
vitro,
knockdown
artery
ECs
led
a
reduction
pSTAT1
pSTAT2
expression.
These
results
suggest
that
promotes
phosphorylation,
enabling
their
translocation.
Additionally,
indicated
positive
correlation
between
cell-specific
molecule
1
(ESM1)
STAT1/STAT2.
Using
UCSC
JASPAR
databases,
multiple
binding
sites
for
STAT1::STAT2
dimer
on
ESM1
promoter.
Luciferase
reporter
assays
revealed
enhanced
following
pSTAT1::pSTAT2
binding,
pinpoint
potential
sites.
Chromatin
Immunoprecipitation
Quantitative
PCR
(ChIP-qPCR)
further
confirmed
specific
findings
highlight
critical
role
IRF5-pSTAT1::pSTAT2-ESM1
pathway
pathogenesis
SSH
therapeutic
targets.
Language: Английский
Fibromyalgia: Chronic Pain Due to a Blood Dysfunction?
International Journal of Molecular Sciences,
Journal Year:
2025,
Volume and Issue:
26(9), P. 4153 - 4153
Published: April 27, 2025
Fibromyalgia
(FM)
is
a
common
chronic
disorder
with
pain.
FM
generally
affects
all
ages
and
occurs
more
commonly
in
women.
The
cause
of
remains
undefined,
but
number
factors
suggest
the
cardiovascular
system
blood
particular
as
contributors
to
its
occurrence
maintenance.
Hemograms
other
indexes
often
show
high
percentages
values
at
'normal',
low,
or
limits
several
outside
'normal'
ranges.
On
hand,
vessels
regulate
arrival
tissues
depending
on
many
internal
external
factors.
Both
aspects
can
interfere
tissue
oxygenation
then
numerous
consequences
induced
by
hypoxia.
In
this
narrative
review,
efforts
were
made
highlight
that
are
potentially
able
affect
oxygen
cells,
well
related
elements
play
role
pain
experienced
patients.
Data
strongly
indicate
most
symptoms
present
patients
find
their
physio-pathological
basis
blood,
suggesting
blood-related
interventions
these
Language: Английский