Immunomodulatory Potential of Kaempferol Isolated from Peronema canescens Jack. Leaves Through Inhibition of IL-6 Expression
International Journal of Molecular Sciences,
Journal Year:
2025,
Volume and Issue:
26(7), P. 3068 - 3068
Published: March 27, 2025
Sungkai
leaves
were
selected
due
to
their
herbal
medicine
prevalence
and
documented
biological
activities.
This
study
explores
the
immunomodulatory
potential
of
kaempferol
isolated
from
(Peronema
canescens
Jack.)
through
a
combination
in
silico
vitro
methods.
P.
extracted
with
ethanol
using
maceration,
followed
by
fractionation
n-hexane,
ethyl
acetate,
water
separatory
funnel.
Among
all
fractions,
acetate
fraction
demonstrated
strongest
inhibitory
effect
on
IL-6
(Interleukin
6)
expression,
leading
further
separation
for
enhanced
analysis
its
activity.
The
resulting
sub-fractions
purified
vacuum
liquid
chromatography
n-hexane
gradient.
Sub-fraction
E
was
preparative
thin-layer
obtain
pure
compound
identified
as
UV,
FTIR,
MS,
NMR
analyses.
then
evaluated
molecular
docking
dynamics
simulations,
employing
MM-PBSA
(Molecular
Mechanics
Poisson-Boltzmann
Surface
Area)
binding
affinity
calculations.
Kaempferol
showed
(ΔG)
-5.98
kcal/mol,
slightly
stronger
than
TLA
(tartaric
acid)
(-5.90
kcal/mol).
Key
interactions
amino
acid
residues,
such
Gln175,
Arg182,
Arg179,
observed.
Additionally,
simulation
that
exhibited
better
stability
between
15
ns
100
ns.
has
strong
van
der
Waals
(-17.02
kcal/mol)
electrostatic
(-293.16
kcal/mol),
free
energy
(-17.85
significantly
(-1.00
stability,
combined
ability
reduce
expression
vitro,
highlights
kaempferol's
potential.
Language: Английский
The Potential Therapeutic Role of Beta-Caryophyllene as a Chemosensitizer and an Inhibitor of Angiogenesis in Cancer
Molecules,
Journal Year:
2025,
Volume and Issue:
30(8), P. 1751 - 1751
Published: April 14, 2025
The
natural,
highly
lipophilic
bicyclic
sesquiterpenes,
Beta-Caryophyllene
(BCP),
was
highlighted
in
several
recent
preclinical
studies
to
enhance
chemo-sensitization
chemo-resistant
tumors
and
efficiently
inhibit
angiogenesis
cancer
cells’
ability
invade
metastasize.
Previous
have
researched
the
reasons
for
synergistic
effect
of
combination
therapy
its
role
as
a
chemosensitizer
an
inhibitor
through
investigating
involved
mechanisms
signaling
molecules.
These
include
nature
BCP,
selective
interaction
BCP
with
CB2,
binding
affinity
receptor
sites
at
angiogenic
vascular
endothelial
growth
factor,
upstream
on
JAK1/STAT3
pathway
other
pathways.
Herein,
enhancing
inhibiting
metastasize
are
highlighted.
appears
be
promising
candidate
treating
when
co-supplemented
drugs
such
cisplatin,
gemcitabine
sorafenib.
Clinical
trials
needed
validate
potential
therapeutic
co-supplementary
drug
therapy,
helping
sensitize
response
drugs,
modulating
pathways,
lowering
drugs’
doses
besides
working
anti-angiogenetic
drug.
Language: Английский