Bioactive Hydroxyapatite–Carboplatin–Quercetin Coatings for Enhanced Osteointegration and Antitumoral Protection in Hip Endoprostheses DOI Open Access

Gheorghe Iosub,

Dana-Ionela Tudorache,

Ionuț Marinel Iova

et al.

Coatings, Journal Year: 2025, Volume and Issue: 15(4), P. 489 - 489

Published: April 20, 2025

The recurrence of bone cancer poses severe complications, particularly after orthopedic surgery, necessitating advanced biomaterials with dual functionality. This study develops nanostructured coatings composed hydroxyapatite, carboplatin, and quercetin, designed to enhance regeneration while delivering localized therapy. These present a promising solution for hip endoprostheses, addressing osteointegration tumor prevention simultaneously. Hydroxyapatite was synthesized characterized using XRD, TEM, SAED, FTIR, SEM assess crystallinity, surface morphology, functional groups. were obtained by MAPLE. In vitro biocompatibility tests showed that HAp@CPT HAp@CPT/QUE supported osteoblast viability adhesion exhibiting selective cytotoxic effects on osteosarcoma cells. Griess assay indicated nitric oxide (NO) levels remained unchanged in hFOB osteoblasts, confirming neither coating induced inflammatory responses healthy contrast, MG63 cells exhibited significantly elevated NO (p < 0.05) response HAp@CPT/QUE, suggesting increased oxidative stress. MTT results 12% 28% reduction cell respectively. Phase-contrast microscopy further confirmed strong reduced attachment, surfaces. findings highlight the functionality hydroxyapatite–carboplatin–quercetin coatings, promoting exerting anticancer effects. Their bone-regenerative properties make them material endoprostheses oncological applications.

Language: Английский

Antioxidant and Anti-Inflammatory Effects of Bioactive Compounds in Atherosclerosis DOI Open Access
Ştefan Horia Roşian,

Ioana Boarescu,

Paul-Mihai Boarescu

et al.

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(3), P. 1379 - 1379

Published: Feb. 6, 2025

Atherosclerosis, a chronic inflammatory disease characterized by the accumulation of lipids and immune cells within arterial walls, remains leading cause cardiovascular morbidity mortality worldwide. Oxidative stress inflammation are central to its pathogenesis, driving endothelial dysfunction, foam cell formation, plaque instability. Emerging evidence highlights potential bioactive compounds with antioxidant anti-inflammatory properties mitigate these processes promote vascular health. This review explores mechanisms through which compounds-such as polyphenols, carotenoids, flavonoids, omega-3 fatty acids, coenzyme Q10, other natural compounds-modulate oxidative in atherosclerosis. It examines their effects on key molecular pathways, including inhibition reactive oxygen species (ROS) production, suppression nuclear factor-κB (NF-κB), modulation cytokines. By integrating current knowledge, this underscores therapeutic dietary supplemental complementary strategies for managing atherosclerosis, paving way future research clinical applications.

Language: Английский

Citations

3
Investigation of the Immunomodulatory and Neuroprotective Properties of Nigella sativa Oil in Experimental Systemic and Neuroinflammation DOI Open Access

Anita Mihaylova,

N. Doncheva,

Maria Vlasheva

et al.

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(5), P. 2235 - 2235

Published: March 2, 2025

Nigella sativa (NS) is a promising medicinal plant with diverse therapeutic properties. This study aimed to investigate the impact of NS oil (NSO) on memory functions in rats LPS (lipopolysaccharide)-induced neuroinflammation, as well its effect serum levels inflammatory cytokines, neuropeptide Y (NPY) and brain-derived neurotrophic factor (BDNF). Male were divided into four groups: control, LPS-control, LPS+NSO 3 5 mL/kg. Neuroinflammation was induced by single intraperitoneal injection (2 mg/kg). The novel object recognition test (NORT) Y-maze used for evaluation processes. Recognition index (RI) % spontaneous alteration (%SA) registered, respectively. Blood samples TNF-α, IL-1β, IL-10, BDNF, NPY taken. Thymoquinone, active compound oil, detected high-performance liquid chromatography. NSO administration resulted an improvement spatial episodic memory, evidenced increased SA RI compared LPS-control. Treatment led significant reduction pro-inflammatory cytokines NPY, along increase IL-10 BDNF levels, when In conclusion, enhances production regulates pro- anti-inflammatory release, which probably contributes observed cognitive animals experimental neuroinflammation.

Language: Английский

Citations

0
An updated review on the phytochemistry and pharmacological activity of black cumin (Nigella sativa L.) DOI Creative Commons
Raghvendra Pandey, Brijesh Pandey, Atul Bhargava

et al.

Advanced Chinese Medicine, Journal Year: 2025, Volume and Issue: unknown

Published: March 23, 2025

Abstract In this review, a comprehensive and systematic evaluation of the phytochemical constituents, traditional medicinal applications, current pharmacological research, toxicity, nanobiotechnology black cumin has been undertaken. An exhaustive database retrieval was conducted to collect scientific information about Nigella sativa L. from 1956 2025 using PubMed, Scopus, ISI Web Science, SciFinder, CABI. Search carried out keywords “ ,” oil,” “Ethnobotany,” “Phytochemistry,” “Pharmacological activity,” “Toxicity,” “Nanotechnology.” Several important phytochemicals are found in seeds N . L., many which seem have good impact on human health put use Ayurvedic, Unani, Siddha systems medicine. Among active compounds, thymoquinone is most important, forming 30%–48% oil fraction. Besides thymoquinone, wide range such as thymohydroquinone, dithymoquinone, p ‐cymene, sabinene, carvacrol, 4‐terpineol, kaempferol (glucoside) t ‐anethol, longifolene (sesquiterpene), α ‐pinene, ‐hederin (pentacyclic triterpene), thymol also reported. Current research provided evidence for uses cumin, especially antioxidant, anti‐inflammatory, antibacterial, antifungal, antiviral, anticancer, antidiabetic, neuroprotective, gastroprotective, antiarthritic effects. The review provides an in‐depth analysis phytochemistry, well potential properties Since plant being extensively investigated its properties, valuable up‐to‐date status will act reference future applications plant.

Language: Английский

Citations

0
Bioactive Hydroxyapatite–Carboplatin–Quercetin Coatings for Enhanced Osteointegration and Antitumoral Protection in Hip Endoprostheses DOI Open Access

Gheorghe Iosub,

Dana-Ionela Tudorache,

Ionuț Marinel Iova

et al.

Coatings, Journal Year: 2025, Volume and Issue: 15(4), P. 489 - 489

Published: April 20, 2025

The recurrence of bone cancer poses severe complications, particularly after orthopedic surgery, necessitating advanced biomaterials with dual functionality. This study develops nanostructured coatings composed hydroxyapatite, carboplatin, and quercetin, designed to enhance regeneration while delivering localized therapy. These present a promising solution for hip endoprostheses, addressing osteointegration tumor prevention simultaneously. Hydroxyapatite was synthesized characterized using XRD, TEM, SAED, FTIR, SEM assess crystallinity, surface morphology, functional groups. were obtained by MAPLE. In vitro biocompatibility tests showed that HAp@CPT HAp@CPT/QUE supported osteoblast viability adhesion exhibiting selective cytotoxic effects on osteosarcoma cells. Griess assay indicated nitric oxide (NO) levels remained unchanged in hFOB osteoblasts, confirming neither coating induced inflammatory responses healthy contrast, MG63 cells exhibited significantly elevated NO (p < 0.05) response HAp@CPT/QUE, suggesting increased oxidative stress. MTT results 12% 28% reduction cell respectively. Phase-contrast microscopy further confirmed strong reduced attachment, surfaces. findings highlight the functionality hydroxyapatite–carboplatin–quercetin coatings, promoting exerting anticancer effects. Their bone-regenerative properties make them material endoprostheses oncological applications.

Language: Английский

Citations

0