Role of Ca2+/calmodulin and PI3K/AKT signaling pathways and active ingredients of BaoTaiYin in treatment of recurrent miscarriage DOI Creative Commons
Ji Li,

Anqi Deng,

Huiying Chen

et al.

Frontiers in Molecular Biosciences, Journal Year: 2025, Volume and Issue: 12

Published: March 25, 2025

Introduction BaoTaiyin (BTY) is a traditional Chinese medicine decoction. It has been used to treat recurrent miscarriage (RM). However, there are no comprehensive systematic studies identify the chemical compositions of BTY and molecular mechanisms on RM. Finding components clarifying underlying processes in treatment RM were goals study. Methods We ultra-high-performance liquid chromatography coupled with triple quadruple time-of-flight tandem mass spectrometry analyze BTY, network analysis predict pharmacological effects identified active ingredients, cell experiments potential mechanisms. Results found 12 ingredients among 61 BTY. These activities linked regulatory 127 key signaling pathways, targeting 107 proteins. Through analysis, we determined that insulin-like growth factor 1 receptor, matrix metalloproteinases, PI3K, STAT3 may be core targets BTY’s therapeutic further explored this mechanism find aqueous extracts significantly enhanced IGFBP2 CaMKK2 expression trophoblast proliferation, whereas inhibitors IGF1R/PI3K/AKT pathway or blocked effect proliferation. In addition, siRNA suppressed BTY-induced expression. Caffeic acid, as one increased intracellular Ca 2+ concentration proliferation trophoblast. Conclusion Our research showed have benefits through multiple such /calmodulin pathways.

Language: Английский

Role of Ca2+/calmodulin and PI3K/AKT signaling pathways and active ingredients of BaoTaiYin in treatment of recurrent miscarriage DOI Creative Commons
Ji Li,

Anqi Deng,

Huiying Chen

et al.

Frontiers in Molecular Biosciences, Journal Year: 2025, Volume and Issue: 12

Published: March 25, 2025

Introduction BaoTaiyin (BTY) is a traditional Chinese medicine decoction. It has been used to treat recurrent miscarriage (RM). However, there are no comprehensive systematic studies identify the chemical compositions of BTY and molecular mechanisms on RM. Finding components clarifying underlying processes in treatment RM were goals study. Methods We ultra-high-performance liquid chromatography coupled with triple quadruple time-of-flight tandem mass spectrometry analyze BTY, network analysis predict pharmacological effects identified active ingredients, cell experiments potential mechanisms. Results found 12 ingredients among 61 BTY. These activities linked regulatory 127 key signaling pathways, targeting 107 proteins. Through analysis, we determined that insulin-like growth factor 1 receptor, matrix metalloproteinases, PI3K, STAT3 may be core targets BTY’s therapeutic further explored this mechanism find aqueous extracts significantly enhanced IGFBP2 CaMKK2 expression trophoblast proliferation, whereas inhibitors IGF1R/PI3K/AKT pathway or blocked effect proliferation. In addition, siRNA suppressed BTY-induced expression. Caffeic acid, as one increased intracellular Ca 2+ concentration proliferation trophoblast. Conclusion Our research showed have benefits through multiple such /calmodulin pathways.

Language: Английский

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