Time-Course Physiological and Transcriptomic Study Reveals Cadmium Effects on Detoxification, Antioxidation, Apoptosis, and Immunity in the Freshwater Snail Pomacea Canaliculata

Published: Jan. 1, 2025

Language: Английский

Cadmium bioaccumulation and detoxification mechanisms in Pomacea insularum: implications for biomonitoring in freshwater ecosystems

Frontiers in Environmental Science, Journal Year: 2025, Volume and Issue: 13

Published: March 10, 2025

This study aimed to investigate cadmium (Cd) bioaccumulation and detoxification mechanisms in Pomacea insularum , based on specimens collected from 13 field populations Peninsular Malaysia transplantation experiments between polluted unpolluted sites. Cd concentrations were analyzed eight tissues, including the cephalic tentacle (CT), pineal sac (PS), digestive tract (DT), shell, determine their roles metal uptake, storage, excretion. The highest concentration was recorded PS (13.5 mg/kg dry weight), while followed by shells (5.91 indicating its role as a long-term sequestration site. Notably, showed exceptionally high accumulation, suggesting potential sensitive biomarker for prolonged exposure. revealed that snails relocated sites accumulated up 5.22 mg/kg, those transferred environments retained 1.00 6.03 slow depuration tissue-dependent retention. Correlation regression analyses demonstrated significant interactions among soft with filtering organs playing primary detoxification, calcified structures contributed sequestration. These findings highlight P. an effective biomonitor assessing environmental contamination bioavailability, providing insights into both short-term exposure storage. Overall, this reinforces suitability of biomonitoring programs demonstrating ability accumulate retain across different human activities. retention specific particularly PS, highlighting importance targeted tissue analysis pollution assessment. Integrating monitoring strategies can provide valuable data trends, aiding management freshwater ecosystem conservation.

Language: Английский

Overexpression of Tetrahymena Cysteine Synthetase 1 Promotes Cadmium Removal by Biosynthesizing Cadmium Sulfide Quantum Dots in Escherichia coli

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(8), P. 3685 - 3685

Published: April 13, 2025

Heavy metal cadmium causes significant contamination in aquatic ecosystems. The biomineralization of represents a vital biological mechanism for handling stress diverse microorganisms. To improve the capacity by microorganisms environments, Tetrahymena cysteine synthetase 1 (TtCsa1) was overexpressed E. coli. tolerance coli/pET-28a-TtCSA1 to enhanced expressing TtCsa1. Upon addition cysteine, generated more H2S, which reacted with Cd2+ form CdS quantum dots (QDs), resulting stronger fluorescence signal. UV-visible absorption and spectra culture supernatant showed characteristic peaks corresponding QDs. Transmission Electron Microscopy (TEM) images confirmed that formation QDs their agglomeration coli cells. X-ray Diffraction Analysis (XRD) analysis further presence crystalline nature. In rich medium, achieved removal rates 99.5%, 98.2%, 56.5%, 49.4%, respectively, concentrations 0.15, 0.3, 0.45, 0.6 mM within 48 h. simulated wastewater, 99.4%, 94.3%, 90.1%, 89.8%, 0.6, 0.75 12 These results demonstrate overexpressing TtCsa1 can significantly enhance its ability biomineralize medium has potential applications bioremediation environments contaminated heavy metals.

Language: Английский