Integrative Proteomic and Phosphoproteomic Profiling Reveals Molecular Mechanisms of Hypoxic Adaptation in Brandt’s Voles (Lasiopodomys brandtii) Brain Tissue DOI Creative Commons
Panqin Wang,

Yongyan Liu,

Yimeng Du

et al.

Cells, Journal Year: 2025, Volume and Issue: 14(7), P. 527 - 527

Published: April 1, 2025

Rapid ascent to high altitudes by unacclimatized individuals significantly increases the risk of brain damage, given brain’s heightened sensitivity hypoxic conditions. Investigating hypoxia-tolerant animals can provide insights into adaptive mechanisms and guide prevention treatment hypoxic-ischemic injury. In this study, we exposed Brandt’s voles simulated (100 m, 3000 5000 7000 m) for 24 h performed quantitative proteomic phosphoproteomic analyses tissue. A total 3990 proteins 9125 phosphorylation sites (phospho-sites) were quantified. Differentially expressed (DE) analysis revealed that while protein abundance changes relatively modest, levels exhibited substantial alterations, suggesting rapidly regulate structure function through maintain cellular homeostasis under acute hypoxia. Clustering showed most co-expressed non-monotonic responses with increasing altitude, which enriched in pathways related cytokine secretion regulation glutathione metabolism, contributing reduced inflammation oxidative stress. contrast, phospho-sites monotonic changes, phospho-proteins glycolysis vascular smooth muscle contraction regulation. Kinase activity prediction identified nine hypoxia-responsive kinases, four belonging CAMK family. Immunoblot validated CAMK2A consistent predictions, may play a crucial role response. conclusion, work discovered cope hypoxia three key strategies: (1) enhance cerebral blood flow, (2) glycolytic activation increase energy production, (3) neuroprotective mechanisms.

Language: Английский

Integrative Proteomic and Phosphoproteomic Profiling Reveals Molecular Mechanisms of Hypoxic Adaptation in Brandt’s Voles (Lasiopodomys brandtii) Brain Tissue DOI Creative Commons
Panqin Wang,

Yongyan Liu,

Yimeng Du

et al.

Cells, Journal Year: 2025, Volume and Issue: 14(7), P. 527 - 527

Published: April 1, 2025

Rapid ascent to high altitudes by unacclimatized individuals significantly increases the risk of brain damage, given brain’s heightened sensitivity hypoxic conditions. Investigating hypoxia-tolerant animals can provide insights into adaptive mechanisms and guide prevention treatment hypoxic-ischemic injury. In this study, we exposed Brandt’s voles simulated (100 m, 3000 5000 7000 m) for 24 h performed quantitative proteomic phosphoproteomic analyses tissue. A total 3990 proteins 9125 phosphorylation sites (phospho-sites) were quantified. Differentially expressed (DE) analysis revealed that while protein abundance changes relatively modest, levels exhibited substantial alterations, suggesting rapidly regulate structure function through maintain cellular homeostasis under acute hypoxia. Clustering showed most co-expressed non-monotonic responses with increasing altitude, which enriched in pathways related cytokine secretion regulation glutathione metabolism, contributing reduced inflammation oxidative stress. contrast, phospho-sites monotonic changes, phospho-proteins glycolysis vascular smooth muscle contraction regulation. Kinase activity prediction identified nine hypoxia-responsive kinases, four belonging CAMK family. Immunoblot validated CAMK2A consistent predictions, may play a crucial role response. conclusion, work discovered cope hypoxia three key strategies: (1) enhance cerebral blood flow, (2) glycolytic activation increase energy production, (3) neuroprotective mechanisms.

Language: Английский

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