Choroid and Retinal Pigmented Epithelium in Pigs: A Comprehensive Light and Ultrastructural Study DOI Open Access
R. K. Barhaiya, Pawan Kumar

Anatomia Histologia Embryologia, Journal Year: 2025, Volume and Issue: 54(2)

Published: March 1, 2025

This study was conducted on 12 adult pigs of a local mixed breed to examine the histology, histochemistry and ultrastructure choroid retinal pigmented epithelium (RPE). The tissues were fixed in Davidson's Fluid for light microscopy Karnovsky's fluid electron microscopy. Due physiological, anatomical metabolic similarities between humans, are suitable animal model various ophthalmic studies. Bruch's membrane exhibited five distinct layers: basement RPE, inner collagenous layer, elastic lamina, outer layer choriocapillaris. Age-related changes, such as vesicular formation, also observed. Patches collagen fibre lamellae noted stroma, indicating presence vestigial tapetum. apical part intercellular space RPE cells completely sealed off by zonula occludens adherens. Hemidesmosomes not present at basal surface cells. Numerous melanosomes, lipofuscin, mitochondria, phagolysosomes, melanolysosomal bodies, both smooth rough endoplasmic reticulum observed granular cytoplasm RPE. Double-membrane-bound round vesicles cytoplasm, characterised uniform intermembranous central core space, potentially representing liposome-like structures, although this type vesicle has been previously described Overall, demonstrated that have choroid, morphology similar providing valuable reference studies using model. Thus, can be utilised an experimental research.

Language: Английский

Energetic failure and oxidative stress underlie the Prpf31 splicing factor-related mouse phenotype DOI Open Access
Abdallah Hamieh,

Elora Vanoni,

Quentin Rieu

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown

Published: March 4, 2025

Abstract Mutations of ubiquitous PRPF splicing factors represent the second cause retina-specific autosomic dominant retinitis pigmentosa. Prpf31 downregulation decreases phagocytosis mouse and human retinal pigment epithelial (RPE) cells, thus suggesting similar pathogenesis between species. With time, RPE ultrastructure shows signs cellular stress such as cytoplasmic vacuoles. To decipher primary origin -related deleterious processes we first confirmed gradual accumulation protein lipid oxidations. We then showed deregulation in expression levels oxidative endoplasmic reticulum markers well mitochondrial respiratory chain constituants, foremost from 3 months onward. For time analyzed energetic metabolism freshly dissected RPE/choroid, retina peritoneal macrophages, that respiration global energy production were decreased solely +/- cells. Therefore, our results indicate metabolic impairments associated might contribute to cells before affecting retina.

Language: Английский

Citations

0
Choroid and Retinal Pigmented Epithelium in Pigs: A Comprehensive Light and Ultrastructural Study DOI Open Access
R. K. Barhaiya, Pawan Kumar

Anatomia Histologia Embryologia, Journal Year: 2025, Volume and Issue: 54(2)

Published: March 1, 2025

This study was conducted on 12 adult pigs of a local mixed breed to examine the histology, histochemistry and ultrastructure choroid retinal pigmented epithelium (RPE). The tissues were fixed in Davidson's Fluid for light microscopy Karnovsky's fluid electron microscopy. Due physiological, anatomical metabolic similarities between humans, are suitable animal model various ophthalmic studies. Bruch's membrane exhibited five distinct layers: basement RPE, inner collagenous layer, elastic lamina, outer layer choriocapillaris. Age-related changes, such as vesicular formation, also observed. Patches collagen fibre lamellae noted stroma, indicating presence vestigial tapetum. apical part intercellular space RPE cells completely sealed off by zonula occludens adherens. Hemidesmosomes not present at basal surface cells. Numerous melanosomes, lipofuscin, mitochondria, phagolysosomes, melanolysosomal bodies, both smooth rough endoplasmic reticulum observed granular cytoplasm RPE. Double-membrane-bound round vesicles cytoplasm, characterised uniform intermembranous central core space, potentially representing liposome-like structures, although this type vesicle has been previously described Overall, demonstrated that have choroid, morphology similar providing valuable reference studies using model. Thus, can be utilised an experimental research.

Language: Английский

Citations

0