3D bioprinted alginate/gelatin hydrogel: concentration modulated properties toward scar-minimized wound healing DOI
Tian Jiao, Chaofan Sun, Zhuo Wang

et al.

Journal of Biomaterials Science Polymer Edition, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 22

Published: April 16, 2025

The critical shortage of transplantable skin remains a leading cause mortality in patients with severe injuries, driving the demand for advanced 3D-bioprinted constructs. While hydrogel-based bioinks are pivotal tissue engineering, existing systems often fail to simultaneously address biomechanical compatibility, scar suppression, and cell viability. Here, we propose rationally designed sodium alginate/gelatin (SA/Gel) hydrogel platform through composition-property-performance correlation analysis. Systematic characterization revealed that increasing gelatin content (8-12 wt%) enhanced viscosity (by 2.5-fold), compressive modulus (25.6 ± 2.7 kPa 37.9 3.5 kPa), tensile fracture elongation (57.9 4.2% 92.1 1.3%), print fidelity, while reducing degradation ratio (62.8 2.9% 26.4 2.4% at day 14) pore size (128.5 16.6 μm 79.4 19.7 μm). optimized A4G10 formulation exhibited synergistic advantages: (1) dynamic swelling (36.3 0.8%) balanced nutrient permeation structural stability; (2) tunable (47.2% matched neo-tissue formation; (3) anisotropic mechanical properties (compressive 32.2 4.1 kPa, 31.7 3.9 kPa) mimicked native mechanics; (4) sub-100 porous architecture (102.9 12.4 μm) effectively suppressed fibroblast over--proliferation. Remarkably, SA/Gel scaffolds maintained 98% viability (Live/Dead assay) vitro, suppressing fibrotic formation facilitating angiogenesis vivo. This multi-functional system demonstrates unprecedented potential as scar--inhibiting bioink clinical-grade regeneration.

Language: Английский

The microRNA landscape and regulatory network in Clonorchis sinensis-infected hepatocellular carcinoma: implications for tumor progression DOI Creative Commons
Caibiao Wei,

Junxian Chen,

Tim Huang

et al.

Parasites & Vectors, Journal Year: 2025, Volume and Issue: 18(1)

Published: Feb. 21, 2025

Language: Английский

Citations

0
3D bioprinted alginate/gelatin hydrogel: concentration modulated properties toward scar-minimized wound healing DOI
Tian Jiao, Chaofan Sun, Zhuo Wang

et al.

Journal of Biomaterials Science Polymer Edition, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 22

Published: April 16, 2025

The critical shortage of transplantable skin remains a leading cause mortality in patients with severe injuries, driving the demand for advanced 3D-bioprinted constructs. While hydrogel-based bioinks are pivotal tissue engineering, existing systems often fail to simultaneously address biomechanical compatibility, scar suppression, and cell viability. Here, we propose rationally designed sodium alginate/gelatin (SA/Gel) hydrogel platform through composition-property-performance correlation analysis. Systematic characterization revealed that increasing gelatin content (8-12 wt%) enhanced viscosity (by 2.5-fold), compressive modulus (25.6 ± 2.7 kPa 37.9 3.5 kPa), tensile fracture elongation (57.9 4.2% 92.1 1.3%), print fidelity, while reducing degradation ratio (62.8 2.9% 26.4 2.4% at day 14) pore size (128.5 16.6 μm 79.4 19.7 μm). optimized A4G10 formulation exhibited synergistic advantages: (1) dynamic swelling (36.3 0.8%) balanced nutrient permeation structural stability; (2) tunable (47.2% matched neo-tissue formation; (3) anisotropic mechanical properties (compressive 32.2 4.1 kPa, 31.7 3.9 kPa) mimicked native mechanics; (4) sub-100 porous architecture (102.9 12.4 μm) effectively suppressed fibroblast over--proliferation. Remarkably, SA/Gel scaffolds maintained 98% viability (Live/Dead assay) vitro, suppressing fibrotic formation facilitating angiogenesis vivo. This multi-functional system demonstrates unprecedented potential as scar--inhibiting bioink clinical-grade regeneration.

Language: Английский

Citations

0